| Easternsugar Ltd | Information & communication | 2017 |
| Eastwood Scenery Ltd | Construction | 2014 |
| Easycrate Limited | Transport & logistics | 2002 |
| EB Equine Limited | Professional & scientific | 2021 |
| EB Storage Limited | Transport & logistics | 2025 |
| Eccobrand Ltd | Information & communication | 2015 |
| Eclipse Business Dynamics Limited | Extraterritorial | 2004 |
| Eclipse Computing (Holdings) Limited | Financial services | 1988 |
| Eclipse Computing Limited | Information & communication | 1987 |
| Ecodase Ltd | Wholesale & retail | 2025 |
| ED CAN Help Ltd | Information & communication | 2020 |
| Edgeway Architecture Ltd | Professional & scientific | 2023 |
| Edward Baden Group Limited | Real estate | 1999 |
| Edward Baden Holdings Limited | Financial services | 2026 |
| Edward Baden Storage Limited | Financial services | 2026 |
| Edwardsim Ltd | Health & social care | 2025 |
| Efamilycloud Limited | Other services | 2015 |
| EGC Contractors Ltd | Administrative & support | 2020 |
| Eileanach Limited | Professional & scientific | 2013 |
| EJH Automotive Ltd | Wholesale & retail | 2020 |
| Elation Health Therapy Ltd | Real estate | 2012 |
| ELECTRICIAN1 Ltd | Construction | 2016 |
| Elegant Design & Solutions Ltd | Professional & scientific | 2006 |
| Elisyn Solutions Ltd | Real estate | 2026 |
| Elizabeth ANN Equestrian and Canine Products Limited | Manufacturing | 2025 |
| Elizabeth Place Freehold Company Limited | Households | 2012 |
| Elorma UK Ltd | Information & communication | 2021 |
| Elsan Limited | Manufacturing | 1963 |
| Emanations Seminars and Teaching Limited | Education | 2024 |
| Emily&Jacob Ltd | Administrative & support | 2016 |
| Emkiel Ltd | Other services | 2018 |
| ENA Creative Ltd | Information & communication | 2022 |
| Enbritely Limited | Information & communication | 2015 |
| Energista Limited | Professional & scientific | 2008 |
| Engage Ltd | Hospitality | 2005 |
| Englifirm Ltd | Administrative & support | 2010 |
| Eniac Computing Ltd | Information & communication | 2015 |
| Entrepreneurial Team Analytics (ETA) Technologies Ltd | Administrative & support | 2022 |
| Environprint Solutions Limited | Manufacturing | 2007 |
| Envirosure Limited | Manufacturing | 2012 |
| EOS Insights Limited | Professional & scientific | 2018 |
| EP Investment Group Ltd | Administrative & support | 2022 |
| EPI Catering Limited | Hospitality | 2013 |
| Equiclub App Ltd | Information & communication | 2024 |
| Essential Christian | Information & communication | 2008 |
| Esteamed DRY Cleaners (Uckfield) Ltd | Other services | 2021 |
| Eternal Flame Heating Limited | Construction | 2017 |
| EU Trans Ltd | Water & waste | 2019 |
| EVA Bookkeeping Limited | Information & communication | 2022 |
| Evans Pharmacy Services Limited | Administrative & support | 2008 |
| Evelyn Management Ltd | Households | 1997 |
| Eventfill Ltd | Professional & scientific | 2023 |
| Evissen Ltd | Professional & scientific | 2026 |
| Evla Ltd | Information & communication | 2011 |
| Evlacorp Ltd | Professional & scientific | 2012 |
| Evlavit Ltd | Wholesale & retail | 2013 |
| Evolution Glass Limited | Construction | 2008 |
| Evolution Management Consulting Limited | Professional & scientific | 2019 |
| Evolution Partners Capital Limited | Professional & scientific | 2022 |
| Expert Client Ventures Ltd | Professional & scientific | 2017 |
| Expert Intermediaries Group Limited | Financial services | 2016 |
| Express Cladding Ltd | Construction | 2016 |
| Express Luck UK Ltd | Wholesale & retail | 2024 |
| F J Hancock & SON Limited | Construction | 2004 |
| F8 Investments Limited | Real estate | 2022 |
| Facetill Ltd | Information & communication | 2022 |
| Faeq London Ltd | Wholesale & retail | 2025 |
| Fairfield House Vets Limited | Professional & scientific | 2023 |
| Fairfield Trading Company Limited | Wholesale & retail | 2026 |
| Fairholme Court Residents Association (Crowborough) Limited | Households | 1974 |
| Falcon Medical Aesthetics Ltd | Health & social care | 2020 |
| Falmer Court Management Company Limited | Real estate | 2003 |
| Family Life Success Ltd | Wholesale & retail | 2019 |
| FAR From the Media Crowd Ltd. | Professional & scientific | 2015 |
| Farrell Medcomms Ltd | Professional & scientific | 2026 |
| Fast Systems Consultants Limited | Information & communication | 1998 |
| FEL Solutions Limited | Administrative & support | 2026 |
| Feorh Ltd | Agriculture & fishing | 2025 |
| Fermento Mill and Bakery Ltd | Manufacturing | 2026 |
| Fetch Group Ltd | Administrative & support | 2019 |
| Fetch Personnel Ltd | Administrative & support | 2019 |
| FFF Invest Ltd | Financial services | 2023 |
| Fftf Holdings Ltd | Financial services | 2013 |
| Fidelity Lodge Trustee Company Limited | Other services | 2014 |
| Fielden Properties Limited | Real estate | 2017 |
| Figg Drainage and Construction Ltd | Construction | 2025 |
| Filial Therapy United Kingdom Limited | Administrative & support | 2006 |
| Fin.Direction LLP | | 2012 |
| First Born Limited | Wholesale & retail | 2005 |
| Firstbit Ltd | Information & communication | 2016 |
| Five ASH Down Paddock Ltd | Agriculture & fishing | 2021 |
| Five Chimneys Business Park LLP | | 2008 |
| FK Drywall Limited | Construction | 2012 |
| Flab House Ltd | Professional & scientific | 2019 |
| Flag Limited | Administrative & support | 2018 |
| Flatfield Limited | Real estate | 1972 |
| Fletcher Cooke Properties Limited | Households | 2025 |
| Fletcher Lopez Ltd | Administrative & support | 2015 |
| Fletching Farms Limited | Agriculture & fishing | 1959 |
| Fletching Limited | Real estate | 2021 |