| A Ward (Benllech) Ltd | Wholesale & retail | 2025 |
| Anglesey Clean Exteriors Ltd | Administrative & support | 2024 |
| Anglesey Inns Limited | Hospitality | 1983 |
| Anglesey Stoves and Fires Limited | Wholesale & retail | 2017 |
| Beard Insights Ltd | Professional & scientific | 2022 |
| Beetle Juice (UK) Limited | Professional & scientific | 2011 |
| Benllech Management Ltd | Professional & scientific | 2018 |
| Brook Admin Management Services Ltd | Real estate | 2013 |
| Cablemole Limited | Manufacturing | 2025 |
| Create by Jemma Ltd | Other services | 2025 |
| Credu Solutions Limited | Information & communication | 2022 |
| Cromlech Manor Farm Ltd | Hospitality | 2026 |
| Cwmni Glyn CYF | Hospitality | 2023 |
| D Clutton Building and Joinery Ltd | Construction | 2025 |
| Elite GAS & OIL Services Ltd | Manufacturing | 2013 |
| Glan Gors Ltd | Hospitality | 2013 |
| Helen Bonnick Optical Ltd | Health & social care | 2018 |
| Himg Ltd | Wholesale & retail | 2024 |
| J D Hulme & Sons Limited | Construction | 2013 |
| Kelsie Jones Ltd | Hospitality | 2020 |
| KOG Films Limited | Information & communication | 2014 |
| Land Resource Partnership Limited | Construction | 2004 |
| Lanxuan Ltd | Hospitality | 2023 |
| Lloriau Gorau Finest Floors Limited | Construction | 2022 |
| Marine Lithium Systems Limited | Professional & scientific | 2026 |
| Mcnicol Limited | Administrative & support | 2006 |
| Mind Aloud C.I.C. | Wholesale & retail | 2018 |
| MÔN Clean Ltd | Administrative & support | 2023 |
| Nicholas Henderson Ltd | Transport & logistics | 2025 |
| Pace Haven Ltd | Real estate | 2025 |
| Red Squirrel Retreats Ltd | Real estate | 2017 |
| Rhos Farm Caravan Park Ltd | Wholesale & retail | 2017 |
| Safe Hands Cleaning Services Ltd | Administrative & support | 2015 |
| Safe Hands Holiday Management Services Ltd | Other services | 2020 |
| Same Day Motors Ltd | Wholesale & retail | 2026 |
| Simon Keeble Limited | Information & communication | 2018 |
| Step UP Exploration Development Limited | Administrative & support | 2017 |
| Step UP Property Rentals Limited | Real estate | 2019 |
| Take 2 (Studios) Limited | Information & communication | 1993 |
| THE Breeze Hotel Limited | Hospitality | 2021 |
| THE Shed Benllech Limited | Hospitality | 2024 |
| UK Supply Chain Associates Limited | Professional & scientific | 2006 |
| W P Griffiths Ltd | Construction | 2021 |
| Wimsg Ltd | Wholesale & retail | 2026 |
| Wind Hydrogen Limited | Energy | 2020 |