| Ennerdale Investments Limited | Wholesale & retail | 2005 |
| Enspire Clubs Ltd | Arts & recreation | 2026 |
| Enterprise Integration Limited | Professional & scientific | 2013 |
| Environmental and Social Advisory Services Limited | Administrative & support | 2011 |
| Environmental Compliance Online Limited | Other services | 2000 |
| Envisage Brands Ltd | Wholesale & retail | 2019 |
| Epifany Limited | Professional & scientific | 2009 |
| Epoch Build Ltd | Professional & scientific | 2019 |
| Epoch Fit OUT & Joinery Ltd | Professional & scientific | 2019 |
| Epoch Interiors Ltd | Professional & scientific | 2019 |
| Epoch Projects Ltd | Professional & scientific | 2019 |
| Epoch Vision Limited | Wholesale & retail | 2017 |
| Epofore Limited | Professional & scientific | 2009 |
| Eradora Sports Limited | Information & communication | 2017 |
| ERD Accountancy Ltd | Professional & scientific | 2021 |
| Erekanuk Ltd | Wholesale & retail | 2025 |
| ERN Consulting Ltd | Real estate | 2022 |
| ES Management & Accounting Limited | Professional & scientific | 2019 |
| Esdent Health Tourism Ltd | Health & social care | 2017 |
| Eshalon Limited | Professional & scientific | 2023 |
| Esims Limited | Administrative & support | 2008 |
| Etain Aesthetics Limited | Wholesale & retail | 2025 |
| Etheris Ltd | Professional & scientific | 2015 |
| Ethic Productions Limited | Information & communication | 2021 |
| Ethicalminds Ltd | Professional & scientific | 2014 |
| Etolon LLP | | 2015 |
| Eton Shaw Limited | Real estate | 2013 |
| Etrb Holdco Ltd | Real estate | 2022 |
| Eugenio Limited | Real estate | 2022 |
| Euro Cafe 2 Limited | Hospitality | 2020 |
| Euro Services Holdings Limited | Financial services | 2019 |
| Euro Services Property Limited | Real estate | 2020 |
| Eurokor Ltd | Administrative & support | 2008 |
| Europe Car Service Limited | Wholesale & retail | 2013 |
| European Association of Science Editors | Other services | 2000 |
| European Trade and Exhibition Services Limited | Other services | 1993 |
| Evanscourt Limited | Professional & scientific | 2011 |
| Evclassics Ltd | Administrative & support | 2021 |
| Event Consulting Services Limited | Professional & scientific | 2025 |
| Event Management Consulting Ltd | Administrative & support | 2025 |
| Eventmark Ltd | Hospitality | 2025 |
| Events Upon Thames Ltd | Hospitality | 2024 |
| Everest Consulting Group Ltd | Information & communication | 2026 |
| Evernorth Education Ltd | Education | 2021 |
| Everydays J K Ltd | Wholesale & retail | 2017 |
| Everydays Mailbox Ltd | Transport & logistics | 2020 |
| Everydays Stationery Ltd | Wholesale & retail | 2018 |
| Everyme Limited | Wholesale & retail | 2001 |
| Everything About Stitch Ltd | Other services | 2017 |
| Everything AI Ltd | Information & communication | 2025 |
| Evian Roofing and Building Ltd | Construction | 2025 |
| Evidence Bureau Ltd | Professional & scientific | 2016 |
| EVN Source Ltd | Construction | 2021 |
| Evnia Coaching Ltd | Professional & scientific | 2018 |
| Evolution (Woking) Holdings Limited | Professional & scientific | 2013 |
| Evolution (Woking) Limited | Construction | 2013 |
| Evoo Ltd | Wholesale & retail | 2020 |
| Eworboys Consulting Ltd | Professional & scientific | 2023 |
| Executive London Group Limited | Other services | 2001 |
| Exelixis Property Services Ltd | Construction | 2023 |
| Exelixis Services Limited | Manufacturing | 2020 |
| Exforde Court LLP | | 2009 |
| Expert Construction Innovation Ltd | Construction | 2024 |
| Expert Medical Examination Ltd | Health & social care | 2025 |
| Exponential Advisory Partners Limited | Professional & scientific | 2023 |
| Exquisite General Service Ltd | Administrative & support | 2023 |
| Extra Strongman Ltd | Construction | 2017 |
| Extracteduk Ltd | Manufacturing | 2025 |
| EYE Smile Limited | Health & social care | 2009 |
| Eyegenius Ltd | Manufacturing | 2013 |
| Eyewear Trends Ltd | Wholesale & retail | 2014 |
| Eyot Thames UK Ltd | Wholesale & retail | 2020 |
| EZD Strategies Limited | Administrative & support | 2019 |
| Ezoka Limited | Information & communication | 2019 |
| F & S Enterprise London Ltd | Hospitality | 2019 |
| Faam Gallery Ltd | Wholesale & retail | 2019 |
| FAB - the Movie Ltd | Information & communication | 2023 |
| Fabula TV Limited | Information & communication | 2023 |
| Facialcentre Limited | Health & social care | 2019 |
| Fact Worldwide Limited | Administrative & support | 2016 |
| Fair Price Foods Ltd | Wholesale & retail | 2015 |
| Fairfield Tax Partners Limited | Professional & scientific | 2025 |
| Fairholme TW1 Limited | Households | 1998 |
| Faith Hair & Co Limited | Other services | 2026 |
| Families Print Limited | Information & communication | 2017 |
| Fantail Business Development Ltd | Professional & scientific | 2012 |
| Farov Construction Limited | Construction | 2018 |
| Farrgone Ltd | Arts & recreation | 2022 |
| Fashfull Ltd | Wholesale & retail | 2019 |
| Fati London Ltd | Hospitality | 2024 |
| FAV Development Ltd | Professional & scientific | 2026 |
| Fawnstars (IP) Limited | Arts & recreation | 2022 |
| FBL Holdings Limited | Financial services | 2008 |
| FC Cement Limited | Wholesale & retail | 2026 |
| FCB Electrical Ltd | Construction | 2025 |
| Fdps Property Ltd | Real estate | 2024 |
| Federation Against Copyright Theft Limited | Other services | 1982 |
| Feelmyflo Ltd | Information & communication | 2020 |
| Felix Sheil Ltd | Real estate | 2024 |
| Fellow FOX Ltd | Information & communication | 2024 |