| Duchy Health Charity Limited | Health & social care | 1976 |
| Duchy Partitions & Ceilings Ltd | Construction | 2026 |
| Duchy Plant Hire Cornwall Ltd | Administrative & support | 2023 |
| Duchy Plant Hire Devon Ltd | Administrative & support | 2023 |
| Duchy Plant Hire Southwest Ltd | Administrative & support | 2023 |
| Duchy Wills Limited | Other services | 2006 |
| Duck Cove LLP | | 2013 |
| Dunescape Perranporth Ltd | Construction | 2022 |
| Durleigh Gardens Management Company Limited | Households | 2018 |
| DV Commercials and Classics Limited | Wholesale & retail | 2014 |
| DVP (UK) Holdings Limited | Financial services | 2019 |
| DW Properties (South West) Ltd | Real estate | 2021 |
| Dwellworks Europe Limited | Professional & scientific | 2013 |
| Dynamic Developments (SW) Limited | Construction | 2016 |
| Dynamism Ltd | Real estate | 2017 |
| E N Inayat Ltd | Health & social care | 2015 |
| E-Tech Learning Limited | Professional & scientific | 2018 |
| E.J.J. Limited | Other services | 2003 |
| E3 Trails Ltd | Education | 2013 |
| Eagle Europe Limited | Information & communication | 2001 |
| Earlyfuture Limited | Real estate | 1991 |
| Earth Energies Ltd | Construction | 2024 |
| Earthworm Garden Services Ltd | Administrative & support | 2015 |
| East Court Management Company Limited | Households | 2014 |
| East Well 168 Ltd | Hospitality | 2025 |
| Eastmawes Limited | Real estate | 2002 |
| Easy Shop 1 Ltd | Wholesale & retail | 2025 |
| EB Auctions Ltd | Real estate | 2026 |
| EBC Groundworks (SW) Ltd | Construction | 2026 |
| EBC Partnerships Limited | Construction | 2014 |
| Ebpt Limited | Arts & recreation | 2015 |
| Echojet Limited | Wholesale & retail | 2019 |
| Eclectic Adventures Limited | Administrative & support | 2009 |
| Eco Active Arborists Ltd | Agriculture & fishing | 2014 |
| Ecodrive Ltd | Professional & scientific | 2012 |
| Ecokirk Ltd | Professional & scientific | 2011 |
| ED Crossley & Associates Limited | Professional & scientific | 2010 |
| ED Harvey Engineering Limited | Manufacturing | 2008 |
| Eden Garden Paradise Limited | Hospitality | 2016 |
| Eden Motorhomes Limited | Wholesale & retail | 2015 |
| Edgcumbe Court Management Company Limited | Real estate | 1987 |
| Edge Construction Cornwall Ltd | Construction | 2025 |
| Edgebranch Limited | Real estate | 1995 |
| Edgware Associates Limited | Administrative & support | 2012 |
| Editor's Lifestyle Limited | Real estate | 2022 |
| EDS Engineering (South West) Limited | Professional & scientific | 2019 |
| Edward Bence Property Ltd | Real estate | 2015 |
| Edward Buckland Limited | Professional & scientific | 2013 |
| Edwardes Developments Limited | Construction | 2012 |
| Edwin Inayat Group Limited | Information & communication | 2025 |
| Effgen Ltd | Professional & scientific | 2019 |
| EGP Distribution Ltd | Wholesale & retail | 2025 |
| EGP Holdings Ltd | Real estate | 2021 |
| Ehcornwall Ltd | Wholesale & retail | 2024 |
| EJM Creative Ltd | Information & communication | 2021 |
| Ekspres Cars Truro Ltd | Transport & logistics | 2024 |
| Elbury Design Limited | Professional & scientific | 2013 |
| Electrical Concepts (SW) Limited | Wholesale & retail | 2007 |
| Electronic Music Studios Ltd | Manufacturing | 2019 |
| Elegant Homes (South West) Limited | Wholesale & retail | 1998 |
| Elements Build SW Ltd | Construction | 2022 |
| Elephant Rock Event Management Limited | Hospitality | 2012 |
| Elephant Tree Consulting Ltd | Professional & scientific | 2015 |
| Elephant Tree Investments Ltd | Households | 2017 |
| Elevate Offshore Limited | Administrative & support | 2022 |
| Eliot Gardens (ST Austell) Management Company Ltd | Households | 2021 |
| Elite 3MS Limited | Other services | 2026 |
| Elite Building & Maintenance SW Ltd | Construction | 2026 |
| Elite Fitness (SW) Limited | Arts & recreation | 2015 |
| Elite Perfection Ltd | Administrative & support | 2024 |
| Elizabeth Court (Bugle) Management Company Limited | Households | 2003 |
| Elizar Limited | Health & social care | 2018 |
| Elliot Lawrie Interior Renovations Limited | Construction | 2009 |
| Ellis Aviation Limited | Arts & recreation | 2006 |
| ELM Court Gardens Limited | Households | 1978 |
| ELS Cornwall Limited | Health & social care | 2025 |
| Elusu Ltd | Professional & scientific | 2015 |
| Elvan Capital Limited | Financial services | 2020 |
| Embankment Malpas Ltd | Construction | 2021 |
| Emge & Co Ltd | Administrative & support | 1993 |
| Emge Consultants Ltd | Professional & scientific | 2020 |
| Emge Paper and Packaging Consultants Ltd | Extraterritorial | 2020 |
| Eminence.V.D Limited | Administrative & support | 2025 |
| Emob Properties Limited | Construction | 2021 |
| Empowered Woman Academy Ltd | Health & social care | 2025 |
| EMS Synthesisers Ltd | Manufacturing | 2019 |
| Endeva Group Limited | Real estate | 2021 |
| Energized Property Ltd | Construction | 2019 |
| Energlow Electrical and Renewables Ltd | Construction | 2025 |
| Energy Performance Southwest Limited | Professional & scientific | 2011 |
| Engineered Earth Ltd | Extraterritorial | 2013 |
| Engineering & Development Solutions Limited | Professional & scientific | 2016 |
| Enjoy 2023 Limited | Wholesale & retail | 2023 |
| Enjoy Falmouth Ltd | Wholesale & retail | 2016 |
| Enjoy Street Cart Limited | Wholesale & retail | 2010 |
| Enodoc Holdings Ltd | Financial services | 2019 |
| Enston Electrical Ltd | Construction | 2025 |
| Enterprise Boats Limited | Extraterritorial | 2012 |
| Enviroad Ltd | Professional & scientific | 2011 |
| Environmental Design Solutions UK PVT Ltd | Information & communication | 2024 |