| Corraith Holdings Ltd | Financial services | 2018 |
| Costley & Costley Hoteliers Limited | Hospitality | 1990 |
| Costley Holdings Ltd | Hospitality | 2019 |
| Costley Inns & Wine Bars Limited | Hospitality | 1996 |
| CPR35 Ltd | Professional & scientific | 2017 |
| Craig Russell Ltd | Health & social care | 2013 |
| Crannag Properties Ltd | Real estate | 2017 |
| Crowlin Property Limited | Real estate | 2021 |
| Cupcake Bouquets Ltd | Manufacturing | 2024 |
| CVS Burnham Ltd. | Hospitality | 1961 |
| D & H Building & Maintenance Services Ltd | Manufacturing | 2025 |
| D J Boyle Limited | Construction | 2017 |
| D R Bookkeeping Ltd | Professional & scientific | 2021 |
| D R Wood Creations Ltd | Manufacturing | 2025 |
| D W Shaw Ltd. | Professional & scientific | 2011 |
| Dark Side of the Moon Limited | Administrative & support | 2014 |
| Darley Laundry Ltd | Other services | 2016 |
| Davari Properties Limited | Real estate | 1988 |
| DBV Cyber Limited | Information & communication | 2024 |
| Dcaddis Ltd | Administrative & support | 2024 |
| Dclean Ltd | Administrative & support | 2023 |
| DD Foods Ltd | Hospitality | 2023 |
| Dege Consulting Ltd | Information & communication | 2012 |
| Designer Garden Rooms & Landscapes Limited | Administrative & support | 2022 |
| Designer Garden Rooms Limited | Construction | 2021 |
| Designy Ltd | Professional & scientific | 2025 |
| Dgpa Limited | Hospitality | 2021 |
| DHF Investments Limited | Real estate | 2023 |
| DKJ Air Ltd | Manufacturing | 2026 |
| DM Consulting Ltd | Information & communication | 2012 |
| Dmblue Limited | Wholesale & retail | 2006 |
| Dogar's Kitchen Ltd | Hospitality | 2020 |
| Dolfer Holding Ltd | Wholesale & retail | 2025 |
| DR Batra Ltd | Health & social care | 2023 |
| Duncan Macpherson Limited | Wholesale & retail | 2019 |
| Dunse Contracts Limited | Professional & scientific | 2007 |
| Dutch FOX Ltd | Real estate | 2019 |
| DW Consulting Services Ltd | Professional & scientific | 2023 |
| Dynamic LED Solutions Ltd | Extraterritorial | 2025 |
| Eglinton Financial Planning Ltd | Professional & scientific | 2026 |
| Electric Bikepost Limited | Arts & recreation | 2020 |
| Elite Engineering Ayrshire Limited | Real estate | 2024 |
| Elthamvend Limited | Real estate | 1975 |
| EMW Coaching Ltd | Other services | 2020 |
| Energy and Retrofits Limited | Construction | 2024 |
| Eolas Global Ltd | Professional & scientific | 2019 |
| Ericas Consulting Limited | Information & communication | 2025 |
| Evanyrouse UK Ltd | Wholesale & retail | 2017 |
| Ewan Mullin Ltd | Professional & scientific | 2023 |
| Ewcom Ltd | Professional & scientific | 2017 |
| Ewing Property Partners Ltd | Real estate | 2023 |
| Expert Drug Witness Services Ltd | Administrative & support | 2017 |
| F-Tech.Tech Limited | Manufacturing | 2016 |
| Factotum Films Ltd | Information & communication | 2024 |
| Fairway Properties Ayrshire Limited | Real estate | 2025 |
| Fast Move Removals & Clearances Ltd | Transport & logistics | 2024 |
| Fernhaven Limited | Real estate | 2001 |
| FH Fire Safety Solutions Ltd | Construction | 2024 |
| Fiducia Financial Services Ltd | Financial services | 2020 |
| Filler Trading Limited | Hospitality | 2025 |
| Finesse Marquees Ltd | Administrative & support | 2012 |
| Flexwave Medical Limited | Professional & scientific | 2007 |
| FMN Solutions Ltd | Manufacturing | 2014 |
| FNR Delivery Ltd | Wholesale & retail | 2024 |
| Forrest Grump Ltd | Professional & scientific | 2026 |
| Foundation Totai | Health & social care | 2006 |
| Foxglide Sportswear Ltd | Manufacturing | 2022 |
| Fresh AYR Folk Fest CIC | Arts & recreation | 2022 |
| Frontline Consultancy Ltd | Professional & scientific | 2020 |
| G.B. Land Management Ltd. | Agriculture & fishing | 2005 |
| G3 Property Limited | Construction | 2013 |
| Gannet Products Ltd | Manufacturing | 2022 |
| Garvens Ltd | Professional & scientific | 2017 |
| GB Precision Plumbing & Heating Ltd | Construction | 2020 |
| GB Soundz Events & Wedding Services Ltd | Arts & recreation | 2022 |
| GBK Properties Ltd | Financial services | 2023 |
| Gdrk Marketing & Consulting Services Limited | Professional & scientific | 2025 |
| Genanalytics Ltd | Professional & scientific | 2015 |
| Genpra GP Websites Ltd | Professional & scientific | 2024 |
| George Bicket & Company Limited | Real estate | 1947 |
| Ghkm Limited | Professional & scientific | 2020 |
| Gillett & Sibert Limited | Manufacturing | 1974 |
| Gillian Baillie Planning Limited | Professional & scientific | 2023 |
| Ginger Glow Ltd | Professional & scientific | 2023 |
| Glendoe Limited | Energy | 2012 |
| Glenfairn (2008) Limited | Financial services | 2008 |
| Glenfairn Holdings Limited | Professional & scientific | 2007 |
| Glennon Bros. Energy Ltd | Energy | 2016 |
| Glennon Bros. Troon Ltd | Real estate | 2009 |
| Glennon Brothers (UK) Limited | Manufacturing | 1920 |
| Global Fluid Controls Ltd | Professional & scientific | 2020 |
| GO2 Invest Ltd | Real estate | 2023 |
| Golden Online Sales Limited | Wholesale & retail | 2025 |
| Gometra LLP | | 2003 |
| Goose and Co Property Ltd | Real estate | 2025 |
| GS Foods Troon Ltd | Administrative & support | 2019 |
| Gtars Ltd | Wholesale & retail | 2022 |
| H Johnstone & SON Limited | Construction | 2003 |
| H&M Groundwork’S Ltd | Construction | 2025 |
| H.E.L. Pension Trustees Limited | Other services | 2013 |