| Christhetics Ltd | Other services | 2021 |
| Ciac Ltd | Hospitality | 2025 |
| City Phone Repairs Ltd | Wholesale & retail | 2025 |
| CJ Reilly Ltd | Information & communication | 2017 |
| Clark Decorating Limited | Construction | 2025 |
| CLC Properties Limited | Real estate | 2025 |
| Cleen-Rite 2000 Limited | Administrative & support | 1987 |
| Cleland Solutions LP | | 2013 |
| Cloud UK Services Ltd | Administrative & support | 2022 |
| Coachline Limited | Professional & scientific | 2011 |
| Coast Kitchens & Bathrooms Limited | Construction | 2014 |
| Cockmuircroft Limited | Administrative & support | 2022 |
| Common Ground Consultancy Ltd | Professional & scientific | 2026 |
| Community Barter Ltd | Information & communication | 2023 |
| Companion Collective Ltd | Information & communication | 2026 |
| Concept Homecare Ltd | Health & social care | 2021 |
| Cousland Park Ltd | Energy | 2011 |
| CR Technology Consultancy Ltd | Information & communication | 2025 |
| Craig Cabs | | 1999 |
| Craig Cameron Inspection Services Limited | Professional & scientific | 2022 |
| Craig Goodluck Joinery Ltd | Construction | 2021 |
| Crawford Property Services Ltd | Hospitality | 2023 |
| Crerar Ltd | Professional & scientific | 2019 |
| Cronin Taxis Limited | Transport & logistics | 2020 |
| Cruz Holdings Co Limited | Real estate | 2026 |
| CSW Security Ltd | Professional & scientific | 2017 |
| CUT Price Timber Limited | Wholesale & retail | 2016 |
| Cute Wave Ltd | Information & communication | 2024 |
| CW Builders Ltd | Construction | 2017 |
| CW Power Ltd | Manufacturing | 2024 |
| D & L Stoves and Fireplaces Ltd | Construction | 2022 |
| D Black Electrical Services Ltd | Construction | 2026 |
| Daily Delight Limited | Manufacturing | 2023 |
| Dailyplanit Ltd | Information & communication | 2025 |
| Daly Groundworks Limited | Construction | 2025 |
| Daniel Pyper Ltd | Agriculture & fishing | 2021 |
| David Hall & SON Ltd. | Water & waste | 2002 |
| David Mcmurdo Couriers Ltd | Transport & logistics | 2026 |
| Deemclothing Limited | Professional & scientific | 2025 |
| Delviny Limited | Wholesale & retail | 2024 |
| Denholm Potato Growers Limited | Agriculture & fishing | 1984 |
| Denis Cadzow and Company Limited | Agriculture & fishing | 1959 |
| Desserts & Jerk Ltd | Hospitality | 2026 |
| Dewar Cyber Consulting Ltd | Information & communication | 2021 |
| Different for One Limited | Wholesale & retail | 2023 |
| DNF Electrical Limited | Extraterritorial | 2022 |
| Dornoom LP | | 2015 |
| Doubletwin LLP | | 2023 |
| Drinks on the Green Ltd | Hospitality | 2020 |
| Drivings Cool Limited | Education | 2004 |
| DS Trees Limited | Agriculture & fishing | 2025 |
| Dual Scan Ltd | Real estate | 2009 |
| Dunbar MOT Centre Ltd | Wholesale & retail | 2022 |
| Dunedin Facilities Management Limited | Professional & scientific | 2012 |
| Durja Retail Limited | Wholesale & retail | 2018 |
| E.Booth&Co Realty Ltd | Real estate | 2024 |
| Eagle Courier & Delivery Services Ltd | Transport & logistics | 2020 |
| East Air Conditioning Ltd. | Construction | 2003 |
| East and West Events Photography East Ltd | Professional & scientific | 2017 |
| East Coast Brewing Company Ltd | Hospitality | 2020 |
| East Coast Sports Cars (Mots) Ltd | Wholesale & retail | 2018 |
| East Coast Sports Cars Ltd | Wholesale & retail | 2023 |
| East Lothian Logistics Ltd | Transport & logistics | 2023 |
| East Lothian Plastering Ltd | Construction | 2026 |
| East Lothian Rope Access Limited | Professional & scientific | 2019 |
| East Mains Limited | Real estate | 2000 |
| Eastside Strength Limited | Arts & recreation | 2024 |
| Eco Collective Ltd | Wholesale & retail | 2025 |
| Eco Pressure Clean Ltd | Administrative & support | 2021 |
| Edinburgh Consulting Ltd | Information & communication | 2017 |
| Edinburgh Door Solutions Ltd | Construction | 2025 |
| Edinburgh People Ltd | Arts & recreation | 2017 |
| Elara Beauty Limited | Other services | 2025 |
| Elcarim Property Ltd | Real estate | 2025 |
| ELEV8HER Ltd | Wholesale & retail | 2025 |
| Elphinstone Tower Limited | Hospitality | 2023 |
| Embervault Global Ltd | Construction | 2026 |
| Emerald Taxis Ltd. | Professional & scientific | 2009 |
| EPI Interventions Ltd | Health & social care | 2014 |
| ETW Recruitment Ltd | Construction | 2018 |
| Everlace Floristry and Events Ltd | Hospitality | 2025 |
| Evolation Limited | Arts & recreation | 2022 |
| Expert Claim Solutions Limited | Financial services | 2015 |
| Eydent Distillers Ltd | Manufacturing | 2020 |
| Ezstreetraves Ltd | Hospitality | 2022 |
| F Finlayson and Sons Plumbing & Tiling Ltd | Construction | 2024 |
| Fairway (Scot) Limited | Construction | 2012 |
| Faodail Ltd | Administrative & support | 2024 |
| Farmlytics Ltd | Extraterritorial | 2014 |
| Farragon Management Ltd | Professional & scientific | 2009 |
| FBS Metalworks Limited | Manufacturing | 2012 |
| Fearless Personal Training Ltd | Arts & recreation | 2026 |
| FH Taxis Ltd. | Transport & logistics | 2001 |
| Fidra+Mabel Ltd | Manufacturing | 2020 |
| Fine Travels PVT Ltd | Other services | 2025 |
| Floral Ambitions Ltd | Wholesale & retail | 2024 |
| Floydcanfixit Ltd | Other services | 2025 |
| Fluke Apparel Limited | Wholesale & retail | 2020 |
| Foodflow CIC | Hospitality | 2025 |
| Fore Property Holdings Ltd | Real estate | 2019 |