| 5SPACES Limited | Real estate | 2026 |
| A World in Miniature Limited | Extraterritorial | 2002 |
| Aaah Developments Limited | Real estate | 2026 |
| AB 3RD Sector Limited | Professional & scientific | 2020 |
| AB Property and Holiday Lettings Ltd | Real estate | 2023 |
| AB Wales Holdings Ltd | Financial services | 2024 |
| Aero Engine Management Services Ltd | Professional & scientific | 2006 |
| Agren Limited | Professional & scientific | 2014 |
| AL-2 Teknik UK Limited | Water & waste | 2018 |
| Allison & Hunter OIL Limited | Wholesale & retail | 2003 |
| Andrew V Hamilton Ltd | Agriculture & fishing | 2025 |
| Armstrong Joinery Products Limited | Manufacturing | 2002 |
| Auchencheyne Limited | Agriculture & fishing | 1974 |
| Beinn IME Hydropower Limited | Energy | 2025 |
| Bluapple Limited | Transport & logistics | 2002 |
| Breadalbane Estate Limited | Agriculture & fishing | 2012 |
| Carruthers Farming | Agriculture & fishing | 2022 |
| Carsphairn Community Woodland Limited | Agriculture & fishing | 2018 |
| Chapters of Thornhill Ltd | Wholesale & retail | 2024 |
| Chesney's K9 Care Limited | Other services | 2021 |
| Clay Works Studios CIC | Education | 2022 |
| Constellation Points Ltd. | Arts & recreation | 2015 |
| Coulson & Tennant Productions Limited | Information & communication | 2018 |
| Country Pubs (Scotland) Limited | Real estate | 2014 |
| Craiglour Limited | Energy | 2015 |
| D & G Hooftrimming Ltd | Agriculture & fishing | 2019 |
| Damian Scott Limited | Wholesale & retail | 1997 |
| DEV Media Design Ltd | Professional & scientific | 2026 |
| Euchanhead LLP | | 2010 |
| Falkland Wool Growers Limited | Wholesale & retail | 1997 |
| Fleming Fencing & Forestry Limited | Manufacturing | 2009 |
| Forest Asset Management Ltd | Agriculture & fishing | 2025 |
| Fortis Shelters Ltd | Public administration | 2025 |
| G&J Micros Limited | Information & communication | 2001 |
| Glencairn Land and Woods Trust Ltd. | Agriculture & fishing | 1998 |
| Greengair Limited | Extraterritorial | 2015 |
| Guano Films Limited | Information & communication | 2024 |
| H.A.L.P. Limited | Hospitality | 1995 |
| H2O Hydro Ltd | Agriculture & fishing | 2013 |
| Hester Holdings Limited | Real estate | 2019 |
| Hill Street 105 Limited | Real estate | 2019 |
| Hiraeth Rentals Limited | Real estate | 2021 |
| Homer Young Design Ltd | Professional & scientific | 2022 |
| IAM Commercial Management Ltd | Professional & scientific | 2018 |
| IDP Consulting Limited | Manufacturing | 2022 |
| Infosec-It Ltd | Other services | 2014 |
| IS Technology Limited | Information & communication | 2023 |
| JAC Design Limited | Professional & scientific | 2007 |
| Jade Garden Thornhill Ltd | Hospitality | 2022 |
| JAH Transport Ltd | Transport & logistics | 2025 |
| JL Roofing Services Limited | Construction | 2025 |
| Jrcsh Limited | Hospitality | 2022 |
| K. O. Site Welding Services Ltd | Manufacturing | 2025 |
| K9A2B Ltd | Transport & logistics | 2020 |
| Kingdom Storage Units Ltd | Transport & logistics | 2022 |
| KIT Allen Ltd | Information & communication | 2008 |
| Knockbrack Wind Limited | Real estate | 2023 |
| Korus NDT Limited | Professional & scientific | 2014 |
| KPT Community Energy and Efficiency Limited | Energy | 2022 |
| KPT Community Trading Ltd | Energy | 2021 |
| KPT Development Trust | Other services | 2018 |
| Lean Bean Limited | Wholesale & retail | 2024 |
| Leask Properties Ltd | Real estate | 2005 |
| Lift Parts and Services Ltd | Manufacturing | 2024 |
| Linn & Ford Limited | Real estate | 1994 |
| M a Radcliffe (Coshogle) & Co | | 2001 |
| M Ayres Roofing Services Limited | Construction | 2016 |
| M.K. Welding (New Cample) Limited | Professional & scientific | 2006 |
| Matrixglen Gundogs Ltd | Agriculture & fishing | 2022 |
| Mckenzie-Wilson Pet Foods Limited | Manufacturing | 2023 |
| Mckill Maxwell & Company Limited | Agriculture & fishing | 1950 |
| Merit 20 Limited | Hospitality | 2020 |
| Messrs. Bain Dunaverig | | 2001 |
| Mlran Ltd | Wholesale & retail | 2026 |
| Morar Design Limited | Professional & scientific | 2013 |
| Nate's Forge Ltd | Manufacturing | 2020 |
| Newleaf Forestry Limited | Agriculture & fishing | 2021 |
| Next Level Growth Ltd | Professional & scientific | 2025 |
| Nith Valley Blade Tech Ltd | Manufacturing | 2022 |
| Nith Valley Free Range Eggs Limited | Agriculture & fishing | 2011 |
| Nithsdale Farm Services Ltd | Agriculture & fishing | 2018 |
| Nithsdale Farm Vets Ltd | Professional & scientific | 2020 |
| Nithsdale Properties Ltd | Financial services | 2020 |
| North Corbelly Dairying | Agriculture & fishing | 2020 |
| P. Fergusson (LPG Engineering) Limited | Manufacturing | 2005 |
| Paddletech Limited | Manufacturing | 2016 |
| Pegasus Fitness Limited | Arts & recreation | 2015 |
| Penpont Holiday Park Limited | Hospitality | 2017 |
| Peter Gronbjerg Limited | Professional & scientific | 2013 |
| Pink Barn Properties Limited | Real estate | 2025 |
| Plbr Ltd | Health & social care | 2025 |
| Project KDN Ltd | Wholesale & retail | 2022 |
| Pyrodry Ltd | Professional & scientific | 2022 |
| Queensberry Farming Limited | Agriculture & fishing | 2017 |
| R & a Mckinley Ltd | Agriculture & fishing | 2012 |
| R Anderson (Ardoch) | | 2001 |
| R D Weir & Company | | 1977 |
| R W Osborne & Co. (Chapel Farm) | | 2000 |
| Reid Rose Strategy and Research Ltd | Professional & scientific | 2019 |
| RJ Livestock Systems Limited | Professional & scientific | 2016 |