| DLP Creative Ltd | Hospitality | 2023 |
| DM Scientific Limited | Professional & scientific | 2009 |
| Dog and GUN Knayton Limited | Hospitality | 2024 |
| Dog and GUN PUB Ltd | Hospitality | 2025 |
| Donna Adams Consulting Limited | Information & communication | 2025 |
| Douglas Barker Ltd | Real estate | 2016 |
| Douglas Dalby Construction Limited | Construction | 2016 |
| DR Soph Aesthetics Limited | Other services | 2023 |
| Dragon Facility Management Ltd | Households | 2025 |
| Dragon Training Ltd | Administrative & support | 2023 |
| Drain Local Limited | Extraterritorial | 2006 |
| DRH Property Management Ltd | Real estate | 2021 |
| Drust Entertainment Ltd | Information & communication | 2023 |
| DS York Ltd | Transport & logistics | 2021 |
| DT Building Services Ltd | Construction | 2021 |
| Dtms Limited | Administrative & support | 2002 |
| Dubarry Homes Ltd. | Construction | 2016 |
| Ducis True North Consulting Ltd | Professional & scientific | 2025 |
| Duffek Consulting Ltd | Professional & scientific | 2014 |
| Duvetset Ltd | Wholesale & retail | 2025 |
| DWR Equine Limited | Professional & scientific | 2014 |
| Earley Ornamentals Limited | Agriculture & fishing | 2009 |
| Easton Builders (Bagby) Ltd | Construction | 2016 |
| Eastwood Packaging Group Ltd | Manufacturing | 2026 |
| Echor Group Ltd | Professional & scientific | 2022 |
| Educationscape Limited | Education | 2010 |
| Edwin Snow Quality Solutions Limited | Professional & scientific | 2011 |
| EKT Pre-Plaster Specialist Ltd | Construction | 2025 |
| Electrical Rewinding Services (York) Limited | Manufacturing | 1961 |
| Elemental Horticultural Services Ltd | Administrative & support | 2024 |
| Elevate Multi Academy Trust | Education | 2017 |
| Elliemaieasby Limited | Hospitality | 2023 |
| Emco Support Ltd | Health & social care | 2026 |
| Empowering Performance Travel Limited | Administrative & support | 2025 |
| Emrys Studio Limited | Wholesale & retail | 2026 |
| Enarby Consulting Limited | Professional & scientific | 2022 |
| Enatasia Equestrian Limited | Wholesale & retail | 2018 |
| Endeavour 159 Limited | Extraterritorial | 2024 |
| Enterpen Limited | Construction | 2008 |
| Enviro-Brew Ltd | Wholesale & retail | 2025 |
| Equine Health Ltd | Manufacturing | 2024 |
| Equipped Gym Limited | Wholesale & retail | 2024 |
| Ergo Trades Academy Limited | Education | 2026 |
| Ermewood Limited | Financial services | 2018 |
| Essence Developments Ltd | Construction | 2014 |
| Essetre UK Limited | Wholesale & retail | 1985 |
| Essjay Property Ltd | Construction | 2021 |
| Euclid Court Management Company Limited | Households | 1988 |
| Eufloria Flowers Ltd | Wholesale & retail | 2023 |
| Euro-Worldwide Trading Limited | Information & communication | 2013 |
| Eurotech Thirsk Limited | Wholesale & retail | 2012 |
| Event Traffic Control Ltd | Transport & logistics | 2013 |
| Event Traffic Management Limited | Transport & logistics | 2026 |
| Evolution Design & Build (Yorkshire) Limited | Construction | 2016 |
| EVOLVE76 Ltd | Real estate | 2026 |
| Excel Draincare Limited | Administrative & support | 2004 |
| Executional Ltd | Other services | 2007 |
| Exit Three Ltd | Hospitality | 2012 |
| EY UP Holdings Limited | Households | 2023 |
| F & L Property Limited | Real estate | 2018 |
| Facade Engineering Technology Ltd | Professional & scientific | 2019 |
| Fahrenheit Farrant Limited | Manufacturing | 2025 |
| Fallow Design Limited | Professional & scientific | 2013 |
| Fallow Developments (Yorkshire) Limited | Construction | 2022 |
| Fallow Property Limited | Construction | 2016 |
| Falsum Customs Consulting Limited | Professional & scientific | 2023 |
| Falsum Group Limited | Financial services | 2023 |
| Falsum Labs Limited | Information & communication | 2022 |
| Farm Clean Ltd | Other services | 2021 |
| Farne House Management Company Limited | Households | 2022 |
| Fast Bits Limited | Information & communication | 2026 |
| Faye Levi Marketing Ltd | Professional & scientific | 2021 |
| FBD Consolidated Limited | Professional & scientific | 2025 |
| FBD Property Investments Limited | Real estate | 2025 |
| FCP Estates Ltd | Real estate | 2020 |
| Fern & Fell Hideaway Ltd | Hospitality | 2026 |
| FET Holdings Ltd | Financial services | 2022 |
| Finaltable Media Ltd | Information & communication | 2025 |
| Find Happy Healthy Love Ltd | Professional & scientific | 2017 |
| Finis Group Limited | Manufacturing | 2020 |
| Fisher King Publishing Limited | Information & communication | 2010 |
| Five One Two (Wetherby) Ltd | Transport & logistics | 2017 |
| Five Point Consulting Limited | Financial services | 2011 |
| Five Point HR Limited | Professional & scientific | 2014 |
| Flete Limited | Financial services | 2018 |
| Flexible Machine Products Limited | Manufacturing | 1998 |
| Flock Plan Consulting Limited | Professional & scientific | 2026 |
| Flow Rider Ltd | Education | 2025 |
| Flowsource Process Fittings Limited | Manufacturing | 2020 |
| Fluid Financial Solutions Ltd | Professional & scientific | 2025 |
| Flumeware Limited | Other services | 2013 |
| Flying FOX Aviation Ltd | Administrative & support | 2018 |
| Fontus Environmental Limited | Professional & scientific | 2017 |
| Fordy Marshall Limited | Administrative & support | 2012 |
| Four Business Consultants Ltd | Professional & scientific | 2025 |
| Four Thirsk LLP | | 2018 |
| FOX (Mouldings) Limited | Manufacturing | 1988 |
| Fox's Signs and Trophies Ltd | Wholesale & retail | 2019 |
| Foxstitch Ltd | Manufacturing | 2016 |
| FPS Flexibles UK Ltd | Manufacturing | 1999 |