| Dolaugu Limited | Professional & scientific | 2011 |
| Domichek Limited | Real estate | 2013 |
| Dominic Parry Limited | Professional & scientific | 2005 |
| Domus Properties Limited | Real estate | 2005 |
| Donald Keith Limited | Real estate | 2021 |
| Donnington Consulting Ltd | Professional & scientific | 2021 |
| Dougie's Diary Ltd | Information & communication | 2014 |
| Down Farm Management Company Limited | Households | 2018 |
| Downe House Foundation | Education | 2014 |
| Downe House International (Oman) Limited | Education | 2019 |
| Downe House International Limited | Education | 2019 |
| Downe House School | Education | 1991 |
| Downe House School Services Limited | Hospitality | 1991 |
| Downes Auto Services Ltd | Wholesale & retail | 2017 |
| Downs Homes Ltd | Real estate | 2025 |
| DPL Financial Limited | Financial services | 2018 |
| Dragonfly Investment Limited | Professional & scientific | 2020 |
| Dream Make Ltd | Real estate | 2019 |
| Dreams Driving Ltd | Education | 2018 |
| Dreamscape Property Ltd | Real estate | 2024 |
| Driscoll Lighting Limited | Information & communication | 2011 |
| Drive Line Marine Limited | Manufacturing | 1994 |
| Driving Clean Limited | Administrative & support | 2019 |
| Drowe Transport Limited | Transport & logistics | 2022 |
| Drvc Bars Limited | Hospitality | 2023 |
| DRY Dental Care Limited | Health & social care | 2018 |
| DRY Ventures Limited | Information & communication | 2015 |
| DS Training and Consultancy Limited | Administrative & support | 2010 |
| Dsign 2 Print Limited | Manufacturing | 2022 |
| DSM Construction & Management Ltd | Manufacturing | 2025 |
| Dsouza Software Consultancy Ltd | Information & communication | 2022 |
| DTW Auto Services Ltd | Wholesale & retail | 2024 |
| Duneguild Limited | Households | 1985 |
| Duzzer Transport Limited | Transport & logistics | 2010 |
| DW Consultancy and Training Limited | Professional & scientific | 2016 |
| Dweck Designs Ltd | Professional & scientific | 2009 |
| Dwellvest Limited | Real estate | 2017 |
| DWS Technical Services Ltd | Professional & scientific | 2019 |
| Dyrham Properties Limited | Real estate | 2014 |
| Dziva Investments Ltd | Real estate | 2020 |
| E & S Group Investments Limited | Financial services | 2021 |
| E & S Investments Limited | Real estate | 2020 |
| E C P Studios Limited | Professional & scientific | 1999 |
| E FIX Roofing Ltd | Construction | 2025 |
| Eagle Golf Simulations Ltd | Arts & recreation | 2023 |
| Earnit Group Ltd | Wholesale & retail | 2026 |
| East Berks. Electrical Limited | Construction | 1975 |
| Eastwick Elger Limited | Real estate | 2021 |
| EAT Well Express Ltd | Hospitality | 2025 |
| EB Europe Limited | Manufacturing | 2016 |
| EBE Engineering Ltd | Professional & scientific | 2006 |
| Ebtv Limited | Administrative & support | 2021 |
| Echo ME Ltd | Information & communication | 2024 |
| Eco Attractions Group C.I.C. | Professional & scientific | 2013 |
| Ecway Limited | Wholesale & retail | 2003 |
| Edge Architecture Ltd | Professional & scientific | 2020 |
| Edge EUC Limited | Information & communication | 2015 |
| Eggleton Construction Ltd | Construction | 2016 |
| Eidolon Limited | Other services | 2017 |
| Electriql Ltd | Manufacturing | 2020 |
| Electronic Manufacturing Solutions Limited | Manufacturing | 2007 |
| Element Electrical Services Limited | Construction | 2020 |
| Eling Developments Limited | Real estate | 2014 |
| Elinor Brett Limited | Other services | 2018 |
| Elite Paving Services Ltd | Construction | 2025 |
| Elite Performance Limited | Other services | 2003 |
| Elizabeth Farm Management Company Limited | Households | 2016 |
| Ellie Osborne Photography Limited | Professional & scientific | 2022 |
| Elliso Security Limited | Information & communication | 2010 |
| Ellison Coast Limited | Other services | 2024 |
| Embergate Investments Ltd | Professional & scientific | 2026 |
| Emma Caswell Ltd | Professional & scientific | 2015 |
| Empty Space Constructions Limited | Real estate | 2019 |
| English Arboretum Limited Partnership | | 2021 |
| Ensol Group Holdings Ltd | Financial services | 2026 |
| Ensol Training and Consultancy Ltd | Professional & scientific | 2026 |
| Enthous Ltd | Information & communication | 2019 |
| Entric Holdings Limited | Financial services | 2011 |
| Envirocorp Ltd | Financial services | 2019 |
| Environmental Solutions Waste Management Ltd | Administrative & support | 2005 |
| Envirovape Ltd | Wholesale & retail | 2018 |
| Envy Thatcham Ltd | Other services | 2020 |
| Equalspace Limited | Real estate | 1986 |
| Equilibrium Lifestyles Ltd | Administrative & support | 2020 |
| Equilibrium Marketing Limited | Professional & scientific | 2015 |
| Equinox Learning Trust | Education | 2011 |
| Esteem Domestic Appliances Ltd | Wholesale & retail | 2013 |
| Esteem Managed Services Limited | Information & communication | 1987 |
| Esteem Software Ltd | Information & communication | 1999 |
| Esteem Trustee Limited | Information & communication | 2002 |
| Ether Events Ltd. | Other services | 2012 |
| Evergreen Sustainability Consulting Limited | Professional & scientific | 2025 |
| EVO Trading Ltd | Wholesale & retail | 2018 |
| Excel 121 Limited | Financial services | 2005 |
| Excilon Ltd | Professional & scientific | 2012 |
| Exclusive Facility Services Ltd | Construction | 2013 |
| Exclusive Refrigerated Trailers Limited | Extraterritorial | 2017 |
| Externiture Limited | Transport & logistics | 2001 |
| EYE 2 EYE Development Ltd | Professional & scientific | 2001 |
| F G Plumbing Services Limited | Construction | 2007 |