| EA Construction OX Ltd | Other services | 2026 |
| EAF Investments Limited | Professional & scientific | 2024 |
| Earth Uniform Limited | Wholesale & retail | 2015 |
| Earthmark Solutions Limited | Information & communication | 2020 |
| Eastcote 93 Limited | Households | 2021 |
| Eastwood Heating Ltd | Construction | 2009 |
| EAT Crisps Ltd | Information & communication | 2024 |
| Echo of Ukraine C.I.C. | Administrative & support | 2025 |
| Eddie Pepperell Golf Ltd | Arts & recreation | 2014 |
| Edge Placemaking Group Ltd | Professional & scientific | 2018 |
| Edge Urban Design Ltd | Professional & scientific | 2018 |
| Edie and PO Ltd | Wholesale & retail | 2020 |
| Edward Hall Classic Mercedes Limited | Wholesale & retail | 2016 |
| EF in Video Limited | Information & communication | 2018 |
| EIGHTEEN86 Ltd | Manufacturing | 2019 |
| EJS Plumbing & Heating Ltd | Extraterritorial | 2022 |
| Electric Gate Expert Ltd | Manufacturing | 2015 |
| Electric Mania Limited | Wholesale & retail | 2010 |
| Electrical and Zero Emissions Limited | Construction | 2021 |
| Elementary Events Ltd | Administrative & support | 2019 |
| Elesec Limited | Construction | 2017 |
| Elite Painting & Decorating Limited | Construction | 2004 |
| Elmatec Limited | Construction | 2020 |
| Elmtec Distribution Limited | Extraterritorial | 2011 |
| ELW Global UK Ltd | Administrative & support | 2010 |
| EM Motorsport Limited | Wholesale & retail | 2003 |
| Emboldened Strategy Limited | Professional & scientific | 2023 |
| Emily's Coffee Limited | Hospitality | 2025 |
| Emmanuelle Walker Ltd | Information & communication | 2019 |
| Emrock Consulting Limited | Education | 2023 |
| Envisage More Ltd | Hospitality | 2025 |
| ERA Clinics Ltd | Health & social care | 2018 |
| ERA Laser Ltd | Health & social care | 2018 |
| Ermington House Ltd | Health & social care | 2016 |
| ESTTIME2020 Ltd | Real estate | 2020 |
| Ethedo Marine Limited | Extraterritorial | 1978 |
| Euclid Energy Ltd | Professional & scientific | 2025 |
| EV Garage Doors and Awnings Limited | Administrative & support | 2025 |
| Everspring Development Limited | Construction | 2023 |
| EVOK3 Performance Ltd | Wholesale & retail | 2019 |
| Excel Finance Solutions Ltd | Financial services | 2008 |
| Exceptional Roofing Ltd | Construction | 2023 |
| Executive Image (Leicester) Limited | Manufacturing | 1999 |
| Executives Online International Limited | Professional & scientific | 1999 |
| Exhale Acupuncture Limited | Health & social care | 2018 |
| Exodus Business Consulting Ltd | Professional & scientific | 2016 |
| Experience Oxfordshire Limited | Administrative & support | 2011 |
| Expression Products Limited | Wholesale & retail | 2013 |
| Extended Mind Ltd. | Education | 2013 |
| F.S. Distribution Limited | Transport & logistics | 1982 |
| Fairfields Consulting Limited | Professional & scientific | 2006 |
| Fairway Construction Southern Limited | Construction | 2019 |
| Falk Consulting Ltd | Information & communication | 2019 |
| Faro Studio Ltd | Professional & scientific | 2017 |
| Farol Limited | Wholesale & retail | 2001 |
| Farrell HR Ltd | Professional & scientific | 2024 |
| Feed ME Light Ltd. | Professional & scientific | 2012 |
| Female Football Nutrition Network Ltd | Professional & scientific | 2024 |
| Fermi Energy Limited | Professional & scientific | 2025 |
| Fibre Engineering Limited | Professional & scientific | 2014 |
| Field Films Limited | Arts & recreation | 1978 |
| FIG Productions Ltd | Information & communication | 2020 |
| Figure 8 Digital Limited | Information & communication | 2016 |
| Figures Partnership Ltd | Professional & scientific | 2026 |
| Financial Aspirations Lifestyle Planning Limited | Financial services | 2006 |
| Financial Partners Group Ltd | Financial services | 2025 |
| Fine Dining Thame Ltd | Hospitality | 2019 |
| Finished to Perfection Ltd | Administrative & support | 2021 |
| Fiona Davenport Family Law Ltd | Professional & scientific | 2023 |
| Firehorse 3 Ltd | Professional & scientific | 2026 |
| Firewalker Productions Ltd | Information & communication | 2016 |
| First Bridge Airports Limited | Transport & logistics | 2026 |
| First Cohort Limited | Professional & scientific | 2019 |
| First Exodus UK Ltd. | Wholesale & retail | 2014 |
| First Life Ltd | Other services | 2021 |
| Fitbox Limited | Other services | 2018 |
| Fitt Development Limited | Construction | 2023 |
| Fitt Properties Limited | Real estate | 1983 |
| Fitter Feet Podiatry Limited | Health & social care | 2014 |
| Fizz Accounting Limited | Professional & scientific | 2007 |
| FJC Property Limited | Real estate | 2026 |
| Flairground Ltd | Arts & recreation | 2026 |
| Flintbrook Property Ltd | Construction | 2025 |
| Flitter Limited | Professional & scientific | 2023 |
| Flooring Product Specialists Limited | Professional & scientific | 2004 |
| Flow-Fix Solutions Ltd | Construction | 2024 |
| Fluisys Ltd | Administrative & support | 2015 |
| Flute Consulting Limited | Other services | 2025 |
| FM Contract Watch LLP | | 2012 |
| Fore Consulting Limited | Professional & scientific | 2025 |
| Former Glory (UK) Limited | Wholesale & retail | 2004 |
| Formula Padel Limited | Arts & recreation | 2025 |
| Fourie Photography Ltd | Professional & scientific | 2020 |
| Foxglades Management Company Limited | Households | 1988 |
| Foxglove Finch Ltd | Wholesale & retail | 2025 |
| Framesync Ltd | Professional & scientific | 2021 |
| Freemanor Limited | Construction | 2014 |
| Freerun PR Limited | Administrative & support | 2016 |
| Frenchip Limited | Education | 2014 |
| Fribeiro Creative Ltd | Professional & scientific | 2024 |