| JSR Autos Ltd | Wholesale & retail | 2012 |
| JT Mukuzisi Limited | Health & social care | 2014 |
| JTR Finance Ltd | Financial services | 2024 |
| Juice IT Solutions Ltd | Information & communication | 2022 |
| Julie Perry & Co Ltd | Professional & scientific | 2020 |
| Jumbles Ltd | Wholesale & retail | 2014 |
| Jumbles Property Ltd | Real estate | 2022 |
| Just a Van? Limited | Manufacturing | 2021 |
| JW Psychology Ltd | Health & social care | 2022 |
| Jwhp Domestic Ltd | Construction | 2022 |
| Jwhp Ltd | Construction | 2022 |
| K & R Homes Limited | Construction | 2025 |
| K and S Transport Specialists Ltd | Transport & logistics | 2019 |
| K Leisure and Entertainment Limited | Arts & recreation | 2021 |
| K Pro Clean Ltd | Administrative & support | 2021 |
| K-Ban Limited | Construction | 2002 |
| K. White Engineering & Engraving Limited | Manufacturing | 2003 |
| K.L.H.A Ltd | Other services | 2023 |
| Kaerospace Limited | Wholesale & retail | 2025 |
| Kamko Trans Ltd | Transport & logistics | 2018 |
| Kash Generator Limited | Professional & scientific | 2015 |
| Kasper Mechanic Limited | Wholesale & retail | 2024 |
| KAT Solutions Limited | Professional & scientific | 2011 |
| Kate Fitch Consultancy Limited | Professional & scientific | 2023 |
| KD Inc Ltd | Professional & scientific | 2024 |
| KDM Transform Limited | Health & social care | 2020 |
| Keighley Imaging Ltd | Health & social care | 2024 |
| Keith Irvine Personal Training Ltd | Arts & recreation | 2016 |
| Kellands (Gloucester) Limited | Financial services | 1999 |
| Kemerton Conservation Trust | Arts & recreation | 1989 |
| Kemper UK & Ireland Limited | Professional & scientific | 2009 |
| KEN Magowan Consulting Ltd | Information & communication | 2020 |
| Kerberos Security Ltd | Administrative & support | 2024 |
| Kevin Fern Photography Limited | Professional & scientific | 2014 |
| Key Brand Associates Ltd | Administrative & support | 2014 |
| KFM Construction Ltd | Construction | 2019 |
| Kinder Law Limited | Professional & scientific | 2018 |
| Kinderoos (Tewkesbury) Ltd | Education | 2012 |
| King Shots Hospitality Ltd | Hospitality | 2023 |
| Kingsbridge Group Limited | Professional & scientific | 2016 |
| Kingsbridge Risk Solutions Limited | Financial services | 2000 |
| Kingsbridge Technology Limited | Information & communication | 2017 |
| Kinvaston Homes Limited | Real estate | 2018 |
| Kiteway Limited | Professional & scientific | 2008 |
| KJ Truckwash Ltd | Transport & logistics | 2025 |
| KKD 2017 Ltd | Transport & logistics | 2017 |
| KLM Commercial Properties Ltd | Construction | 2026 |
| KMS Scaffolding (Gloucestershire) Ltd | Construction | 2025 |
| Kndcleaning Ltd | Administrative & support | 2025 |
| Knight & Smith Property Ltd | Real estate | 2026 |
| Knightedd Limited | Real estate | 2024 |
| Knightsway Properties Ltd | Real estate | 2023 |
| KOA Collective Ltd | Professional & scientific | 2024 |
| Konnected Ltd | Manufacturing | 2018 |
| KRS Lion Bidco Limited | Financial services | 2018 |
| Kudos Housing Limited | Real estate | 2021 |
| Kurdish Barber Shop GL20 Ltd | Other services | 2026 |
| KZW Logistics Ltd | Transport & logistics | 2026 |
| L & D Homes Ltd | Construction | 2024 |
| L C H Plumbing and Heating Ltd | Construction | 2021 |
| L. C. Millar & Sons Limited | Construction | 1976 |
| L3HARRIS TRL Technology Limited | Manufacturing | 1983 |
| LA Gelateria Shop Ltd | Hospitality | 2024 |
| LA VIE EN Rose Limited | Health & social care | 2018 |
| LA VIE EN ROSE24 Ltd | Health & social care | 2018 |
| LAB Hana Consulting Limited | Wholesale & retail | 2026 |
| LAB Hana Limited | Wholesale & retail | 2023 |
| LAB Hana Studio Limited | Wholesale & retail | 2026 |
| Lachecki Consulting Ltd | Professional & scientific | 2010 |
| Lakana Developments Ltd | Construction | 2011 |
| Lakes Corporate Holdings Limited | Wholesale & retail | 2023 |
| Lakes Showering Spaces Ltd | Wholesale & retail | 2021 |
| Lane CTC Limited | Agriculture & fishing | 2026 |
| Langford Tejrar Planning Limited | Professional & scientific | 2018 |
| Lansbury Services Limited | Manufacturing | 2022 |
| Larsen Howie Limited | Financial services | 2015 |
| LASH28 Limited | Other services | 2024 |
| Laskeys Shutters, Curtains and Blinds Ltd | Manufacturing | 2018 |
| Lavacastle Limited | Households | 1986 |
| Lawless Lettings Limited | Real estate | 2024 |
| Lawlesspt Limited | Education | 2024 |
| Layout Automation Ltd. | Information & communication | 1998 |
| LB Management Consultancy Limited | Professional & scientific | 2025 |
| LB Properties & Investments Ltd | Real estate | 2021 |
| LC Paraplanning Ltd | Administrative & support | 2018 |
| LCM Flat Roofing Ltd | Construction | 2019 |
| LCM Property Developments Ltd | Construction | 2021 |
| LCN Inspection Services Ltd | Professional & scientific | 2025 |
| LD Medical Imaging Ltd | Health & social care | 2022 |
| LE Brun Building Contractors Limited | Construction | 2012 |
| Leadbetter Healthcare Limited | Health & social care | 2021 |
| Leading Edge Broking Ltd | Financial services | 2011 |
| Leaf Therapy Ltd | Wholesale & retail | 2022 |
| Learnxyz Limited | Extraterritorial | 2021 |
| Lechlade K9 Ltd | Other services | 2024 |
| Ledbury Wine Limited | Wholesale & retail | 2010 |
| LEE Brewer Ltd | Transport & logistics | 2024 |
| LEE H Properties Ltd | Real estate | 2020 |
| LEE Hawley Photography Ltd | Professional & scientific | 2018 |
| Leett Putting Hub Ltd | Education | 2021 |