| SHB Partners Limited | Administrative & support | 2013 |
| Sheep Street & Co Investments Limited | Real estate | 2018 |
| Shell 123 Ltd | Health & social care | 2011 |
| Sherman Hill Consulting Limited | Professional & scientific | 2013 |
| Shiptinvest NO.1 Limited | Real estate | 2003 |
| Shipton Mill Limited | Manufacturing | 1979 |
| Shirestone Construction Limited | Construction | 2025 |
| Shooters Hill Limited | Administrative & support | 2007 |
| SHS Bricklaying Ltd | Construction | 2025 |
| Sillestica Limited | Manufacturing | 2023 |
| Silver Line FOX Installations Limited | Construction | 2013 |
| SIM2SKY Limited | Other services | 2019 |
| Simon Wareham Consultancy Services Limited | Administrative & support | 2025 |
| Simpson's Cleaning Co. Ltd | Administrative & support | 2024 |
| SIR Halley Stewart Trustee Limited | Extraterritorial | 2022 |
| SJS Tetbury Limited | Administrative & support | 2012 |
| Skyjet Aviation Training Limited | Other services | 2017 |
| Slickeye Limited | Information & communication | 2015 |
| Small World Marketing Limited | Administrative & support | 2005 |
| Smart Designs (Swindon) Limited | Administrative & support | 2013 |
| Sofa Ambiance Ltd | Extraterritorial | 2013 |
| Solarpvcharging Ltd | Energy | 2023 |
| Solvant Limited | Professional & scientific | 2010 |
| Somerford Environmental Limited | Professional & scientific | 2008 |
| Somewhere South Limited | Other services | 2025 |
| South West Electrical Contractors Limited | Construction | 2026 |
| South West Flooring Specialists Limited | Construction | 2024 |
| Spark ED Limited | Education | 2015 |
| Special Effects Training Company Ltd | Arts & recreation | 2019 |
| Specific Gravity Limited | Real estate | 2025 |
| Speedtec Motorsport Limited | Wholesale & retail | 2014 |
| Speedy Iron (Tetbury) Limited | Other services | 2011 |
| Sprint Aviation Training Limited | Other services | 2014 |
| Square One Analysis Limited | Professional & scientific | 2015 |
| ST Julians Building Services Ltd | Administrative & support | 2010 |
| STC Services (Stroud) Limited | Administrative & support | 2005 |
| Steam Powered Services Limited | Professional & scientific | 1992 |
| Steel City Grips Ltd | Administrative & support | 2021 |
| Stenner Bricklaying Contractors Limited | Construction | 2019 |
| Stenner Properties Limited | Administrative & support | 2026 |
| Stingray Marine Technology Ltd | Information & communication | 2024 |
| Stockplus Limited | Wholesale & retail | 2006 |
| Stoneman Masonry Ltd | Construction | 2014 |
| Stones Solutions Ltd | Information & communication | 2023 |
| Stroud Valley Windows Limited | Construction | 2012 |
| Stuart Davey Limited | Professional & scientific | 2016 |
| Stuart Jepps Services Limited | Other services | 2022 |
| Stuart Poole Limited | Administrative & support | 2026 |
| Studio Clement Ltd | Professional & scientific | 2017 |
| Styling 5 Limited | Other services | 2025 |
| Summer Cafe Limited | Wholesale & retail | 2003 |
| Sureteam Ltd | Professional & scientific | 1999 |
| Survival Equipment Services Limited | Transport & logistics | 1998 |
| Sustain Cotswolds CIC | Water & waste | 2025 |
| Sustain Management Consulting Limited | Professional & scientific | 1999 |
| Swindon Bathroom Centre Limited | Wholesale & retail | 2013 |
| Swindon Plumbing Supplies Limited | Wholesale & retail | 1997 |
| SWS and P Ltd | Professional & scientific | 2013 |
| Sycamore Close (Long Newnton) Management Company Limited | Households | 2011 |
| Synchro Management Group Limited | Professional & scientific | 2021 |
| T H and Jones Ltd | Construction | 2023 |
| T J Retail Joinery Limited | Construction | 2010 |
| T&T Recruitment Consultancy Limited | Public administration | 2024 |
| Talbot Management Co Ltd | Households | 2006 |
| Talisman One to One Limited | Hospitality | 2002 |
| Tallmarc Consulting Ltd | Professional & scientific | 2019 |
| TAVERN21 Ltd | Hospitality | 2020 |
| TDI Digital Limited | Information & communication | 2022 |
| Team Perry’S Motor Sport Limited | Arts & recreation | 2012 |
| Technical Mouldings Limited | Manufacturing | 2004 |
| Technos Solutions Limited | Information & communication | 2002 |
| Tenpayne Limited | Households | 1984 |
| Tenth Avenue Leisure Ltd | Wholesale & retail | 2016 |
| Terrastyle UK Limited | Administrative & support | 2008 |
| Terry Blyth Plumbing and Heating Limited | Construction | 2019 |
| Terry Rayner Consulting Limited | Professional & scientific | 2021 |
| Tetbury Camping at the Holford Arms Ltd | Extraterritorial | 2018 |
| Tetbury Contractors Ltd | Construction | 2025 |
| Tetbury Cricket Club Limited | Arts & recreation | 2021 |
| Tetbury Forestry Limited | Agriculture & fishing | 2015 |
| Tetbury Hospital Trust Limited | Health & social care | 1992 |
| Tetbury Lions Trust | Other services | 2013 |
| Tetbury Management Solutions Limited | Information & communication | 2008 |
| Tetbury Nails Ltd | Other services | 2024 |
| Tetbury Padel Barn Ltd | Arts & recreation | 2026 |
| Tetbury Plumbing & Heating Company Limited | Construction | 2006 |
| Tetbury Rail Lands Regeneration Trust Limited | Arts & recreation | 2000 |
| Tetbury Sports Bar Limited | Arts & recreation | 2022 |
| Tetbury Upton Water Management Limited | Water & waste | 1999 |
| Tetbury Woolsack Races C.I.C. | Arts & recreation | 2026 |
| Tetfest Limited | Arts & recreation | 2023 |
| Tetreme Investments Limited | Real estate | 2014 |
| TFT - South West Limited | Real estate | 2024 |
| TGN Engineering Limited | Professional & scientific | 2020 |
| THE Anthroposcene Limited | Arts & recreation | 2018 |
| THE Ashley Family Foundation | Health & social care | 1983 |
| THE Athelstan Trust | Education | 2011 |
| THE Badger Property Group Limited | Extraterritorial | 2022 |
| THE Beauty Rooms Medi Spa Franchise Ltd | Other services | 2023 |
| THE Beauty Rooms Medi Spa Ltd | Other services | 2020 |