| Consol Suncenter (London) Limited | Other services | 2007 |
| Cook Estates Ltd | Real estate | 2019 |
| Coolest Bananas Limited | Professional & scientific | 2007 |
| Cope Building Contractors Limited | Construction | 2020 |
| Cornelius Fuel Ltd | Information & communication | 2015 |
| Corry Holdings Ltd | Financial services | 2017 |
| Cossentials Ltd | Wholesale & retail | 2023 |
| Cotswold Brickwork Limited | Construction | 2018 |
| Cotswold Building & Roofing Ltd | Construction | 2020 |
| Cotswold Car Hub Limited | Wholesale & retail | 2019 |
| Cotswold Clinical Services Limited | Health & social care | 2026 |
| Cotswold Energy Consulting Ltd. | Professional & scientific | 2022 |
| Cotswold Fire & Security Limited | Administrative & support | 2019 |
| Cotswold Fit for YOU Limited | Professional & scientific | 2014 |
| Cotswold Home Developments Ltd | Real estate | 2021 |
| Cotswold Motorsport Limited | Wholesale & retail | 2014 |
| Cotswold Pet Ltd | Wholesale & retail | 2026 |
| Cotswold Private Investigations Ltd | Administrative & support | 2020 |
| Cotswold Racing Ltd | Arts & recreation | 2022 |
| Cotswold Steel Stockholders Limited | Wholesale & retail | 1985 |
| Cotswold Stone Supplies Limited | Mining & quarrying | 1996 |
| Cotswold Thresholds Ltd | Manufacturing | 2012 |
| Cotswold Vehicle Services Limited | Wholesale & retail | 2015 |
| Cotswold Wealth Solutions Ltd | Financial services | 2015 |
| Cotswolds Flooring Group Ltd | Wholesale & retail | 2025 |
| Cotswolds Learning Limited | Education | 2020 |
| Courier Republic Limited | Transport & logistics | 2016 |
| Courtney Louise Photography Limited | Professional & scientific | 2016 |
| Craig Royd Limited | Real estate | 2021 |
| Crescar Holdings Limited | Financial services | 2025 |
| Crescar Partners Project Management Services UK Limited | Transport & logistics | 2022 |
| Crux Electrical Ltd | Other services | 2025 |
| CSC Windows and Doors Limited | Construction | 2023 |
| Curly Wurly Ltd | Information & communication | 2016 |
| Current Electrical Supplies Limited | Wholesale & retail | 2012 |
| CW Business Technology Ltd | Information & communication | 2022 |
| CWP Construction Ltd | Construction | 2025 |
| D a H Carpentry Limited | Administrative & support | 2006 |
| D F Sales Limited | Wholesale & retail | 2006 |
| D.J.H. Flooring Limited | Wholesale & retail | 2009 |
| D.R. Apew Limited | Construction | 2006 |
| D.S.D. Construction Group Ltd | Construction | 2025 |
| Daedalus Aviation Consultancy Ltd | Professional & scientific | 2023 |
| DAILY33 Limited | Information & communication | 2025 |
| Dalmore Pastures Limited | Agriculture & fishing | 2025 |
| Datalise Consulting LLP | | 2020 |
| Dataloaf Ltd | Information & communication | 2014 |
| David James Food Limited | Wholesale & retail | 1993 |
| David James Ingredients Limited | Manufacturing | 2025 |
| David Pearson Director Limited | Information & communication | 2012 |
| DCW Dentistry Limited | Health & social care | 2019 |
| Deilf Ltd | Real estate | 2020 |
| Demartec Corporation Ltd | Hospitality | 2009 |
| Demco Diesels Limited | | 1989 |
| Denovo Services Limited | Construction | 2016 |
| Devizes Motors Ltd | Wholesale & retail | 2017 |
| Diary Detox Limited | Professional & scientific | 2013 |
| Digbeck Limited | Households | 1980 |
| Diligent Robot Ltd | Information & communication | 2017 |
| Dinner at Ltd | Hospitality | 2020 |
| Discount Tyres of Stroud Limited | Wholesale & retail | 2016 |
| Djai Limited | Other services | 2021 |
| DM UK Solutions Ltd | Professional & scientific | 2022 |
| Dog MUG Limited | Extraterritorial | 2019 |
| Double a Media Limited | Information & communication | 2009 |
| Dowry Maintenance (South West) Limited | Administrative & support | 2005 |
| Dpmb Limited | Administrative & support | 2009 |
| DR Chloe Vandertripp Aesthetics Limited | Health & social care | 2026 |
| Dragon & Thistle Consulting Limited | Professional & scientific | 2024 |
| Drizzle Chocolate Limited | Wholesale & retail | 2014 |
| DTP Software Limited | Wholesale & retail | 2007 |
| E. & J. Phillips Limited | Real estate | 1979 |
| EA Cleaning Services Ltd | Administrative & support | 2025 |
| Edgerton Enterprises Limited | Other services | 2013 |
| Edgworks Limited | Manufacturing | 2020 |
| Edward Drury Limited | Construction | 2019 |
| EH Aviation Training Services Limited | Other services | 2015 |
| Elevated Eats Limited | Hospitality | 2024 |
| Elite Auto Bodyworks Limited | Wholesale & retail | 2019 |
| Elliot Noyes Ltd | Construction | 2016 |
| Elliott Brothers (Carpentry & Building) Limited | Construction | 2011 |
| Elsiewood Limited | Transport & logistics | 2013 |
| Elsinore Holdings Limited | Real estate | 2020 |
| Elvire Aesthetics Ltd | Health & social care | 2022 |
| Enarch Ltd | Professional & scientific | 2025 |
| Enco Heating Limited | Construction | 2009 |
| Energise Technology Solutions Limited | Information & communication | 2017 |
| Enthalpy Systems Ltd | Energy | 2022 |
| Envisage Beauty Limited | Other services | 2013 |
| Equiland Limited | Agriculture & fishing | 1997 |
| Esco Trading Ltd | Professional & scientific | 2021 |
| Essential Skills Coaching (UK) Limited | Administrative & support | 2009 |
| Evenheat (UK) Limited | Construction | 1994 |
| Evesham OPS. Ltd | Administrative & support | 2015 |
| Evolution Evolving (Poplar) Limited | Construction | 2022 |
| Evolution Evolving Limited | Construction | 2020 |
| Exceed Health & Fitness Ltd | Education | 2025 |
| Excel Matting Limited | Wholesale & retail | 2025 |
| Expatia Ltd | Information & communication | 2004 |
| Express Polymers (Europe) Limited | Water & waste | 2006 |