| Unit 18 Ltd | Wholesale & retail | 2018 |
| Unit One Developments Ltd | Construction | 2025 |
| Universal Lead Strategies Limited | Other services | 2016 |
| Uniwire Holdings Limited | Financial services | 2002 |
| Uniwire International Limited | Administrative & support | 2014 |
| Uniwire Ltd. | Manufacturing | 1980 |
| Upbeat Media Limited | Information & communication | 2024 |
| Urban Beach Limited | Information & communication | 2004 |
| Urban Embers Limited | Hospitality | 2025 |
| Urras Advisory and Collaboration Limited | Professional & scientific | 2020 |
| Utelcom Limited | Information & communication | 2008 |
| Utelcom UK Limited | Information & communication | 2005 |
| V.D.S Performance Tuning Ltd | Manufacturing | 2021 |
| Valkyrie K9 Ltd | Wholesale & retail | 2024 |
| Value ADD Consultancy Limited | Professional & scientific | 2016 |
| Vanessa Arbuthnott Fabrics Limited | Extraterritorial | 2023 |
| Vanguard Strategy Partners Ltd | Professional & scientific | 2025 |
| Vantage Property Development Ltd | Construction | 2026 |
| Venture Coaching & Consultancy Ltd | Professional & scientific | 2023 |
| Verdant Terra Limited | Professional & scientific | 2024 |
| Verity & Beverley Limited | Professional & scientific | 2002 |
| Viggo Medical Devices Limited | Wholesale & retail | 2010 |
| Vintage Marquees Limited | Other services | 2012 |
| Visara Media Limited | Information & communication | 2019 |
| Voluntiera Ltd | Professional & scientific | 2026 |
| VON Botham Limited | Professional & scientific | 2017 |
| Voyager Digital Limited | Administrative & support | 2012 |
| Vulcan Forged Ltd | Information & communication | 2020 |
| W & a Air Conditioning Contractors Limited | Energy | 2024 |
| W & a Building Contractors Limited | Construction | 2020 |
| W & a Electrical Contractors Limited | Construction | 2021 |
| W & a Electricals Limited | Energy | 2014 |
| W & a Garden Rooms Limited | Construction | 2025 |
| Waggoners Farm Ltd | Agriculture & fishing | 2015 |
| Walbert Holidays LLP | | 2026 |
| Warburton Flight Training Limited | Other services | 2018 |
| Wason & Webb Limited | Construction | 2008 |
| Wattsal Limited | Administrative & support | 1958 |
| WB Interactive Limited | Professional & scientific | 2002 |
| Wedlan Limited | Real estate | 2000 |
| Westminster Liverpool Properties Limited | Real estate | 1990 |
| Westonbirt Management Limited | Households | 2004 |
| Westward Food and Drink Ltd | Manufacturing | 2026 |
| Wetcutz Ltd | Other services | 2008 |
| Whistle Stop Café (Tetbury) Limited | Hospitality | 2018 |
| White Gleam Limited | Health & social care | 2013 |
| Whitebeam Landscapes Ltd | Administrative & support | 2025 |
| Wild Like HER Limited | Wholesale & retail | 2018 |
| Williams Textiles Limited | Manufacturing | 2002 |
| Willow & Co (Cotswolds) Ltd | Hospitality | 2021 |
| Wiltshire Football Academy Limited | Extraterritorial | 2015 |
| Wiltshire Horseboxes Limited | Other services | 2025 |
| Wiltshire Labels Limited | Manufacturing | 2022 |
| Wiltshire Sports Academy Ltd | Administrative & support | 2009 |
| Winkfield Project Management Limited | Households | 2007 |
| Winkfield Property Management Limited | Households | 2006 |
| Women's Farm & Garden Association(The) | Education | 1929 |
| Woodfield Education Ltd | Education | 2026 |
| Woodlands Bloodstock Limited | Arts & recreation | 2015 |
| Woodlodge Holdings Limited | Professional & scientific | 2016 |
| Woodlodge International Limited | Extraterritorial | 1995 |
| Woodlodge Ltd | Extraterritorial | 2019 |
| Woodlodge Products Limited | Wholesale & retail | 1997 |
| Woodworx Carpentry Innovations Limited | Construction | 2018 |
| Wrona Project Management Limited | Construction | 2022 |
| Wronas Renegades Limited | Arts & recreation | 2023 |
| WS Props Ltd | Information & communication | 2019 |
| Xerxes Holdings Limited | Hospitality | 2015 |
| Yome Collective Limited | Wholesale & retail | 2025 |
| YOU Build WE Buy Limited | Wholesale & retail | 2020 |
| YOU ME and the Sea Ltd | Financial services | 2021 |
| Youknitwun Developments Ltd | Construction | 2025 |
| Zarry Investments Limited | Other services | 2022 |
| Zate Films Ltd | Information & communication | 2023 |
| Zexy Ltd | Manufacturing | 2019 |
| ÉCH Apparel Limited | Wholesale & retail | 2019 |