| Dolls Tattoo Limited | Arts & recreation | 2021 |
| Domno. Limited | Wholesale & retail | 2019 |
| Door Nineteen Limited | Professional & scientific | 2021 |
| Doran Family Vintners Limited | Wholesale & retail | 2011 |
| Dory Trans Ltd | Transport & logistics | 2023 |
| DOT COM Digital Limited | Education | 2018 |
| DOT Innovations CIC | Education | 2025 |
| DOT Solutions Ltd | Construction | 2024 |
| Dottie Kitchen Limited | Hospitality | 2013 |
| Doubleboost Limited | Information & communication | 2014 |
| Dough Idea Ltd | Other services | 2015 |
| Douglas and Landmann Limited | Other services | 2007 |
| Dovery Technology Limited | Information & communication | 2016 |
| Down Syndrome International | Information & communication | 2001 |
| Down's Syndrome Association | Health & social care | 1997 |
| Dplusbstudios Limited | Professional & scientific | 2018 |
| Dream Intelligence Ltd | Information & communication | 2025 |
| Drinksmith Consulting Limited | Professional & scientific | 2021 |
| Duck Ventures Limited | Real estate | 2017 |
| Dumari Properties Ltd | Real estate | 2009 |
| Dumpling Productions Limited | Information & communication | 2007 |
| Duncan Foster Architects Ltd | Professional & scientific | 2005 |
| Dunya Builds Ltd | Construction | 2022 |
| Duotraq Limited | Wholesale & retail | 2009 |
| Dutch Courage (Mobile Bar and Events) Ltd | Hospitality | 2021 |
| DWG Business Services Ltd | Real estate | 2013 |
| Dycy Property Limited | Real estate | 2023 |
| Dynamic Link UK Limited | Professional & scientific | 2008 |
| Dynamic Relations Ltd | Information & communication | 2015 |
| E1W Limited | Real estate | 2021 |
| EA2 Limited | Real estate | 2003 |
| Early Years Investments (Downend) Limited | Education | 2014 |
| Early Years Investments (FB) Limited | Education | 2019 |
| Early Years Investments Limited | Education | 2011 |
| Earmax Media UK Limited | Professional & scientific | 2023 |
| Earthbase Limited | Professional & scientific | 2023 |
| Ebba Brasch Limited | Information & communication | 2020 |
| Ebili's Ltd | Other services | 2018 |
| Echlon IT Consulting Limited | Professional & scientific | 2021 |
| Ecocork Coatings LLP | | 2026 |
| Ecocork Ltd | Construction | 2025 |
| Ecoglow Electrical Engineering Ltd | Construction | 2022 |
| Ecoglow NET Zero Ltd | Construction | 2022 |
| Ecoglow Renewable Energy Ltd | Construction | 2022 |
| ECW Ltd | Professional & scientific | 2023 |
| ED and Sara Limited | Arts & recreation | 2011 |
| Eden Goods Ltd | Extraterritorial | 2020 |
| Edgecote Property Limited | Construction | 2024 |
| Edit Magic Limited | Information & communication | 2011 |
| Editable Ltd | Arts & recreation | 2023 |
| Edmond X Construction Ltd | Construction | 2021 |
| Edmond X Developments Ltd | Construction | 2024 |
| Education 365 London Ltd | Administrative & support | 2024 |
| Edwin Doran's Travel World Limited | Real estate | 1974 |
| Efficientfx Ltd | Financial services | 2019 |
| EGG Advisor Ltd | Health & social care | 2023 |
| Eggs Eleven Limited | Hospitality | 2018 |
| Eggs Eleven Trading Limited | Hospitality | 2025 |
| Eggs Trading Limited | Hospitality | 2022 |
| Eggs Twelve Limited | Hospitality | 2020 |
| Eightex Limited | Information & communication | 2017 |
| Eighty Five Limited | Professional & scientific | 2022 |
| Eilidh Dawson Ltd | Professional & scientific | 2025 |
| EL (RET) Community Interest Company | Education | 2020 |
| Eleanor Rose Ltd | Professional & scientific | 2020 |
| Elementary Networks Limited | Information & communication | 2014 |
| Elevare Coaching Limited | Professional & scientific | 2025 |
| Elfin Communications Ltd | Professional & scientific | 2022 |
| Elfin Mansions Management (Teddington) Limited | Households | 1978 |
| Elite Grooming I Ltd | Other services | 2025 |
| Elkinshaw Limited | Information & communication | 2017 |
| ELL4 Holdings Ltd | Financial services | 2025 |
| Elleray Community Association | Other services | 2012 |
| Ellivet Limited | Professional & scientific | 2024 |
| Elmfield Associates Ltd | Professional & scientific | 2008 |
| Elora Health Ltd | Information & communication | 2026 |
| Elucid Partners Limited | Administrative & support | 2021 |
| Emergensys Limited | Information & communication | 2016 |
| Emilio Donoso Limited | Manufacturing | 2015 |
| Emily B Wilson Limited | Arts & recreation | 2015 |
| Emma the Embryologist Ltd | Education | 2024 |
| Emmanuel Teddington | Real estate | 2014 |
| Endre-Tech Limited | Information & communication | 2016 |
| Enexis Ltd | Information & communication | 2012 |
| Enigmax Limited | Professional & scientific | 2005 |
| Ennum Limited | Professional & scientific | 2026 |
| Enso Medical Limited | Health & social care | 2025 |
| Enterprise Connect Solutions Ltd | Information & communication | 2026 |
| Envelop Tech Ltd | Information & communication | 2017 |
| Envied Homes Limited | Construction | 2017 |
| Environmental Equilibrium Limited | Professional & scientific | 2009 |
| Enzed Property Investments Ltd | Real estate | 2022 |
| Eqwelly Ltd | Health & social care | 2019 |
| Esayflow BX Ltd | Wholesale & retail | 2026 |
| Escapiste Projects Limited | Wholesale & retail | 2026 |
| ESI Enterprises Ltd | Wholesale & retail | 2024 |
| Eskape Nights Events Limited | Information & communication | 2025 |
| ESS Construction Limited | Professional & scientific | 2025 |
| Esse Marketing Limited | Professional & scientific | 2020 |
| Estates 17 Limited | Real estate | 2010 |