| Aston Financial Ltd | Financial services | 2006 |
| Aviagen Turkeys Limited | Agriculture & fishing | 1962 |
| Black Dog Coffee Chester Ltd | Wholesale & retail | 2024 |
| Blackham Reclamation LLP | | 2012 |
| Blackhurst Engineering Ltd | Manufacturing | 2016 |
| Blithe House Financial Management Ltd | Professional & scientific | 2014 |
| Buttercup Stables & Trekking Ltd | Agriculture & fishing | 2016 |
| Cheshire Farm Yurts Ltd | Arts & recreation | 2015 |
| Church Bank Homecare Ltd | Health & social care | 2020 |
| Cloud Strider Equestrian Limited | Arts & recreation | 2016 |
| Consultancies Limited | Extraterritorial | 2005 |
| Edge Building Supplies Limited | Wholesale & retail | 2015 |
| English Target Shooting Federation Limited | Arts & recreation | 2003 |
| G J C Instruments Limited | Manufacturing | 1998 |
| Ginger Orchid Limited | Professional & scientific | 2017 |
| JXL Ltd | Arts & recreation | 2018 |
| Kappita Management Ltd | Information & communication | 2014 |
| Keel Boat Services Limited | Construction | 2018 |
| Little Pals Limited | Wholesale & retail | 2006 |
| LOS Dryliners Limited | Construction | 2007 |
| LUV Cars Limited | Professional & scientific | 2025 |
| Martyn & Glanvill Ltd | Professional & scientific | 2024 |
| MGD Building Services Ltd | Construction | 2016 |
| Openside Technology Limited | Information & communication | 2020 |
| Panhurst Limited | Administrative & support | 1970 |
| Paul Jameson Anaesthetics Ltd | Health & social care | 2017 |
| Point North (International) Limited | Wholesale & retail | 2011 |
| Sandy Bears Nursery + Pre-School Limited | Education | 2012 |
| Stem and Silk Limited | Wholesale & retail | 2023 |
| Tattenhall Pubco Ltd | Hospitality | 2023 |
| Village Letz Limited | Real estate | 2022 |