| DS Multi Trade Ltd | Construction | 2021 |
| DT Tech Ltd | Construction | 2012 |
| DTE Networks Ltd | Construction | 2014 |
| Dual Cool and Shopfitting Ltd | Manufacturing | 2020 |
| Dunamis Holdings and Property Limited | Real estate | 2025 |
| Duriancodes Software Solutions Limited | Information & communication | 2019 |
| DVZ Building Services Ltd | Construction | 2019 |
| DXA Ltd | Health & social care | 2016 |
| Dylunio Cymru Limited | Professional & scientific | 2021 |
| Dymchurch Fish Bar Limited | Wholesale & retail | 2021 |
| Dynamik Data Services Limited | Information & communication | 1995 |
| Dyno Group Limited | Financial services | 2016 |
| Dyno Heating (Essex) Limited | Construction | 2014 |
| Dyno Heating (South East London & Kent) Limited | Real estate | 2014 |
| Dyno-Rod (Essex) Limited | Construction | 2015 |
| E-Health Consulting Limited | Information & communication | 2008 |
| E-Portfolio Solutions Limited | Professional & scientific | 2006 |
| E. a. S. Interiors Limited | Construction | 1996 |
| E.M Building and Construction Ltd | Construction | 2017 |
| E3 Group Developments Ltd | Construction | 2026 |
| Eagle Search & Selection Ltd | Other services | 2023 |
| Eagle Swanley Properties Ltd | Real estate | 2022 |
| Eaglepacks Global Limited | Information & communication | 2022 |
| Early Years Childminding Ltd | Health & social care | 2020 |
| EAS Capital Ltd | Wholesale & retail | 2025 |
| East Beeches Property Ltd | Construction | 2020 |
| Eastbury Park (Holdings) Limited | Professional & scientific | 2006 |
| Eastbury Park Limited | Public administration | 2006 |
| Eastern Balance Limited | Education | 2026 |
| Easy Crafts Ideas Ltd | Arts & recreation | 2025 |
| EBS Windows & Doors Limited | Construction | 2020 |
| Eco Friendly Decking Solutions Ltd | Wholesale & retail | 2025 |
| Eco White Ltd | Administrative & support | 2015 |
| Edgar Court Residents' Association Limited | Real estate | 1968 |
| Efficientworks Ltd | Information & communication | 2021 |
| EGG and Matt Creative Limited | Professional & scientific | 2013 |
| EGO Properties Limited | Real estate | 2019 |
| Egor Howell Ltd | Information & communication | 2025 |
| EJW Property Limited | Real estate | 2025 |
| Elahi Associates on Thames Ltd | Professional & scientific | 2010 |
| Elahi Properties on Thames Ltd | Real estate | 2025 |
| Electrified Heat Ltd | Manufacturing | 2023 |
| Elements of Wood Ltd | Wholesale & retail | 2013 |
| Elevation Residences Ltd | Real estate | 2025 |
| Elevation Wellbeing Services Ltd | Information & communication | 2024 |
| Elevationsend Ltd | Financial services | 2025 |
| Elite Air Solutions Limited | Construction | 2021 |
| Elite Property Refurbishment Ltd | Construction | 2025 |
| Ellejay Gold Consultants Ltd | Administrative & support | 2008 |
| Ellenbrook Developments PLC | Administrative & support | 2001 |
| Ellenbrook Holdings Limited | Financial services | 2001 |
| Elliotts Build Ltd | Construction | 2015 |
| Elliotts Developments Ltd | Construction | 2023 |
| Ellis & Jones Fishmonger Ltd | Wholesale & retail | 2019 |
| ELM & IVY Garden Services Limited | Administrative & support | 2025 |
| Elmcroft Business Services Limited | Professional & scientific | 2021 |
| Elms Construction Limited | Construction | 2016 |
| Elne Estates Ltd | Real estate | 2016 |
| Elo's Turismo Ltd | Transport & logistics | 2025 |
| Elvee Limited | Information & communication | 2024 |
| Elwa Holdings Limited | Professional & scientific | 2010 |
| Elwa Limited | Other services | 1992 |
| EM Building Ltd | Construction | 2023 |
| EM Motorworks Ltd | Professional & scientific | 2026 |
| Emperor Elevators Ltd | Professional & scientific | 2026 |
| Endeavour SCH Holdings Limited | Financial services | 1998 |
| Endeavour SCH PLC | Health & social care | 1998 |
| Energair Services Ltd | Construction | 2019 |
| Engracia Partnership Ltd | Real estate | 2024 |
| Enviroflow Drainage Ltd | Water & waste | 2022 |
| Environmental Cooling Solutions Limited | Professional & scientific | 2011 |
| Environmental Social Governance Solutions Limited | Professional & scientific | 2010 |
| Enzymaway Ltd | Wholesale & retail | 2025 |
| Epcot Properties Limited | Real estate | 2022 |
| EPE Division Descendants Union | Extraterritorial | 2006 |
| EPI Construction Limited | Construction | 2015 |
| Equillium Wealth Management Ltd | Financial services | 2025 |
| Equine Bitting Solutions Ltd | Arts & recreation | 2020 |
| Equipped Building Services Limited | Construction | 2016 |
| ES Infotech Limited | Information & communication | 2020 |
| Esohe Ltd | Hospitality | 2018 |
| Esper Developments Ltd | Real estate | 2023 |
| ESS Fleet Services Limited | Wholesale & retail | 2024 |
| Essential IT and Office Supplies Limited | Wholesale & retail | 2020 |
| Esteem Supported Living Ltd | Administrative & support | 2023 |
| Eternal Consult Limited | Education | 2017 |
| Eunice Care Ltd | Health & social care | 2020 |
| Euro Studio UK Ltd | Construction | 2025 |
| Euro VEG Ltd | Administrative & support | 2020 |
| Euro-Soft Developments Limited | Information & communication | 2005 |
| Euston Solar Farm Limited | Other services | 2019 |
| Evercore Solutions Ltd | Wholesale & retail | 2016 |
| Everton Wight Electrical Limited | Construction | 2021 |
| Evolveinvest Limited | Real estate | 2014 |
| Exceldec Limited | Construction | 2020 |
| Exceptional Business Solutions Ltd | Professional & scientific | 2024 |
| Exemplar Financial Services Limited | Financial services | 2021 |
| Exquisite Home Improvements Limited | Construction | 2019 |
| Extreme AI Network Limited | Information & communication | 2023 |
| Extremedental Ltd | Health & social care | 2013 |