| Dream Reality Interactive Limited | Information & communication | 2016 |
| Dream Reveal Limited | Other services | 2018 |
| DSM Design Solutions Limited | Professional & scientific | 2014 |
| DSW Carpentry Limited | Construction | 2011 |
| DTO Properties Limited | Households | 2014 |
| DU Toit and Burger Partnership (Sudbury) Ltd | Health & social care | 2006 |
| Dunn Property Holdings Limited | Real estate | 2025 |
| Dunwell Pro Limited | Construction | 2023 |
| DV Holdings Limited | Professional & scientific | 2015 |
| E a Stretch Events Ltd | Hospitality | 2024 |
| E K K Management Ltd | Real estate | 2020 |
| E L G Suffolk Limited | Real estate | 2025 |
| E-Dno Consulting Ltd | Professional & scientific | 2025 |
| E.C. Wire Limited | Administrative & support | 1996 |
| E.D.S. Engineering Limited | Professional & scientific | 1987 |
| E.L.B. Engineering Limited | Manufacturing | 2017 |
| E.L.G East Ltd | Real estate | 2024 |
| EA Festival Limited | Arts & recreation | 2021 |
| EA Property Holdings Ltd | Real estate | 2019 |
| Eagles of Sudbury UK Limited | Wholesale & retail | 2019 |
| Eaglespur Limited | Professional & scientific | 2013 |
| Eaglespur Properties Limited | Real estate | 2019 |
| Ealing Park Mansions Limited | Real estate | 1985 |
| Earth-Temple Limited | Extraterritorial | 2021 |
| East Anglia Wine Region Ltd | Wholesale & retail | 2024 |
| East Anglian Restoration Ltd | Construction | 2024 |
| East Services Limited | Education | 2001 |
| East Street Daily Convenience Store Ltd | Other services | 2021 |
| East to West Sprinkler Systems Limited | Construction | 2012 |
| Eastburn Technology Consultancy Limited | Information & communication | 1994 |
| EAT Core Ltd | Wholesale & retail | 2026 |
| Eaton Rose Limited | Real estate | 2025 |
| Ebooksstore Limited | Information & communication | 2024 |
| Ecce Limited | Construction | 2025 |
| Echo on Limited | Information & communication | 2024 |
| Eclipse Business Media Limited | Professional & scientific | 2008 |
| Eden's Project | Health & social care | 1999 |
| Eden-Rose Coppice Trust Limited | Health & social care | 2007 |
| Edge Physio Limited | Health & social care | 2019 |
| Edward Gittins & Associates Ltd | Administrative & support | 2001 |
| Edwards Wilding Group Limited | Financial services | 2025 |
| Edwin's Installations Ltd | Construction | 2011 |
| EEC Power Systems Ltd | Energy | 2012 |
| EES Estimating & Engineering Services Ltd | Professional & scientific | 2011 |
| EFX (UK) Limited | Professional & scientific | 1999 |
| Egore Limited | Administrative & support | 2021 |
| EL Nogal 5 Limited | Real estate | 2015 |
| Electrical Devices Limited | Manufacturing | 2004 |
| Elephant Games Limited | Manufacturing | 2000 |
| Elevate Education With Becky Limited | Education | 2025 |
| Elevationvelo Ltd | Wholesale & retail | 2021 |
| Elford & Richford Limited | Construction | 1973 |
| Elford & Sons Limited | Construction | 1983 |
| Elite Suite Stays Ltd | Real estate | 2022 |
| Ellis & Daisy Co Ltd | Real estate | 2022 |
| Elmacik Limited | Hospitality | 2026 |
| Elora Publishing Limited | Information & communication | 2022 |
| EM Wheatley LLP | | 2021 |
| EMG Holdings Limited | Professional & scientific | 1999 |
| Emjay Valves and Controls Limited | Wholesale & retail | 2001 |
| Emma O'Connell Garden Design Limited | Professional & scientific | 2023 |
| Emmerson Critchley Limited | Construction | 2014 |
| Emulsion Sprayer Services Limited | Construction | 2021 |
| EN Physique Gym Limited | Arts & recreation | 2017 |
| Enertech Systems Ltd | Construction | 1997 |
| English College London Limited | | 2005 |
| Engstrom Group Ltd | Professional & scientific | 2018 |
| Ensham Court Management Company Ltd | Real estate | 2013 |
| Ensured Compliance Limited | Financial services | 2018 |
| Enturas Limited | Professional & scientific | 2025 |
| EO Escape Rooms Ltd | Arts & recreation | 2020 |
| ES Shutters and Awnings Ltd | Construction | 2022 |
| Escape to the Country Ltd | Hospitality | 2018 |
| Essex Aquatics Limited | Agriculture & fishing | 2014 |
| Essex Bakery Ltd | Wholesale & retail | 2022 |
| Essex Gate Systems Limited | Manufacturing | 2002 |
| Essex Prestige Homes Limited | Construction | 2014 |
| EST 1420 Ltd | Real estate | 2025 |
| Estaor One Limited | Administrative & support | 2021 |
| Estate Strategy Group Ltd | Professional & scientific | 2012 |
| ETF Ltd | Administrative & support | 2013 |
| ETO Green Property Ltd Ltd | Real estate | 2020 |
| Euclidean Medical Technology Ltd | Professional & scientific | 2025 |
| EUM UK Limited | Financial services | 2001 |
| Euro UK Travel Ltd | Transport & logistics | 2019 |
| Eurosurfacing Limited | Construction | 2015 |
| Eutierra Ltd | Wholesale & retail | 2026 |
| EV & JH Investments Ltd | Financial services | 2022 |
| EVA Court Management Limited | Households | 2008 |
| EVE Original Entertainment Ltd | Arts & recreation | 2026 |
| Event Production Sound Light Limited | Arts & recreation | 2013 |
| Everest Base Camp Ltd | Extraterritorial | 2023 |
| Everest Bites Ltd | Hospitality | 2026 |
| Everest Business Investments Ltd | Wholesale & retail | 2022 |
| Everest Gym Limited | Arts & recreation | 2016 |
| Everflow Heating & Plumbing Ltd | Construction | 2017 |
| Everglow Fields Ltd | Hospitality | 2026 |
| Evergreen Branding Limited | Administrative & support | 2020 |
| Evisla Holdings Ltd | Real estate | 2023 |
| Exeter Capital Limited | Professional & scientific | 2016 |