| BED Head Limited | Information & communication | 2012 |
| Bedwell Landscapes Ltd | Administrative & support | 2008 |
| Beechwood Consultants Limited | Professional & scientific | 2013 |
| Beechwood Design (UK) Ltd | Administrative & support | 2008 |
| Belinay Limited | Hospitality | 2025 |
| Bell Hill Management Limited | Real estate | 2012 |
| Bell House Apartments Limited | Real estate | 2014 |
| Belle VUE Reconstruction Company Limited | Extraterritorial | 2014 |
| Belliniesq Ltd | Wholesale & retail | 2008 |
| Belvedere Management Company Limited | Households | 1986 |
| BEN Living Contractors Ltd | Construction | 2002 |
| BEN Lowe Limited | Arts & recreation | 2015 |
| BEN Pearce Limited | Professional & scientific | 2013 |
| Berbo Design Ltd | Professional & scientific | 2023 |
| Berkeley Interiors Commercial & Domestic Ltd | Construction | 2011 |
| Bertie's Ice Cream Limited | Wholesale & retail | 2022 |
| Bespoke Stone Walling Limited | Construction | 2016 |
| Best Stroud Burger Ltd | Hospitality | 2025 |
| Better Business. Better World Ltd. | Professional & scientific | 2021 |
| Beyond Letterpress Limited | Wholesale & retail | 2018 |
| Bicker FEN Energy Storage Limited | Extraterritorial | 2001 |
| Bicknor Ltd | Households | 2017 |
| Bidmine Limited | Extraterritorial | 1987 |
| Biff Games Ltd | Wholesale & retail | 2020 |
| Bigview Limited | Professional & scientific | 2019 |
| Biocentre Resources Ltd | Water & waste | 2021 |
| Biodynamic Agricultural College | Education | 2010 |
| Biologic Solutions Ltd | Professional & scientific | 2022 |
| Biosouth Energy Limited | Energy | 2024 |
| Biosouth Limited | Energy | 2020 |
| Biotechflow Ltd | Manufacturing | 2010 |
| Biotechflow Production Limited | Manufacturing | 2021 |
| Birch Catering Ltd | Hospitality | 2013 |
| Birch Heating Ltd | Construction | 2019 |
| Birch Restaurant Limited | Hospitality | 2014 |
| Bird & Barrow Ltd | Administrative & support | 2021 |
| Birdcage Books Ltd | Administrative & support | 2013 |
| Bishop's Cleeve Smile Limited | Health & social care | 2019 |
| Bisley Birds Ltd | Agriculture & fishing | 2025 |
| Bisley Community Composting Scheme CIC | Water & waste | 2014 |
| Bitb Property Limited | Real estate | 2025 |
| BJM Assets Limited | Real estate | 2019 |
| BJT Commercial Services Limited | Professional & scientific | 2023 |
| Black ARK Media C.I.C. | Education | 2025 |
| Black FOX Consulting Limited | Professional & scientific | 2012 |
| Black Stag Ltd | Arts & recreation | 2020 |
| Black Swan Thinking Ltd | Professional & scientific | 2024 |
| Blackenstone Limited | Real estate | 1992 |
| Blackwood & Locke Ltd | Manufacturing | 2011 |
| Blades Power Generation Holdings Limited | Real estate | 2023 |
| Blades Power Generation Limited | Professional & scientific | 2013 |
| Blake Services (Stroud) Ltd | Wholesale & retail | 2008 |
| Blakewell Ltd | Financial services | 2022 |
| Blakey Developments Limited | Construction | 2003 |
| Blanc Check Limited | Information & communication | 2011 |
| Blockhaus Cycles Limited | Wholesale & retail | 2017 |
| Blood Red Limited | Manufacturing | 2017 |
| Blue 8 Fire & Security Ltd | Administrative & support | 2019 |
| Blue Green Studio Ltd | Professional & scientific | 2020 |
| Blue Human Project CIC | Health & social care | 2024 |
| Blue M Consulting Ltd | Professional & scientific | 2023 |
| Blue Medical Limited | Professional & scientific | 2025 |
| Blue Nebula Limited | Information & communication | 2021 |
| Blue Ocean Consulting Limited | Professional & scientific | 2018 |
| Blue Willow Coaching Limited | Other services | 2015 |
| Blueboys Garage Limited | Wholesale & retail | 2013 |
| Blueflame Plumbing Ltd | Construction | 2021 |
| Bluefoal LLP | | 2016 |
| Bluehaven Markets Limited | Financial services | 2025 |
| Bluesky Educational Services Ltd | Professional & scientific | 2021 |
| BM Leak Detection Ltd | Water & waste | 2025 |
| BMC Enterprises Ltd | Real estate | 2020 |
| BMK Engineering Limited | | 1991 |
| Bngl Ltd | Manufacturing | 2024 |
| BOB Products Ltd | Construction | 2007 |
| Bobjay Ltd | Professional & scientific | 2014 |
| Bonavista Limited | Wholesale & retail | 2011 |
| Bookcheck Limited | Professional & scientific | 1995 |
| Bookcheck X Ltd | Information & communication | 2021 |
| Bookmark Computers (of Stroud) Limited | Information & communication | 2001 |
| Boon Consultancy Services Ltd | Information & communication | 2016 |
| Border Homes (Southern) Ltd | Real estate | 2001 |
| Boss Morris Ltd | Arts & recreation | 2025 |
| BOT Events Ltd | Arts & recreation | 2025 |
| Boulder Black Holdings Ltd | Financial services | 2026 |
| Boulder Black Ltd | Professional & scientific | 2026 |
| Boundinbliss Limited | Wholesale & retail | 2016 |
| Bowbridge Green Ltd | Professional & scientific | 2013 |
| Bowbridge Stroud Limited | Professional & scientific | 2015 |
| Bowen Consulting Ltd | Information & communication | 2017 |
| Bowmen of Minchinhampton Ltd | Arts & recreation | 2017 |
| Bownham Mead Rodborough Residents' Association Limited | Administrative & support | 1979 |
| Bownham Park Rodborough Residents' Association Limited | Households | 1976 |
| Box & Yard Self Store Limited | Transport & logistics | 2021 |
| Box Kitchen Food Ltd | Hospitality | 2019 |
| BOZ & Company (South West) Ltd | Agriculture & fishing | 2023 |
| Bozzard Fp&A Consultancy Limited | Professional & scientific | 2013 |
| Bpnc Limited | Hospitality | 2021 |
| Brackenfern Advisory Limited | Professional & scientific | 2024 |
| Brackenfern Properties Limited | Real estate | 2026 |