| Adviceon... Ltd | Financial services | 1986 |
| AE (Anthony Edwards) Properties Limited | Real estate | 2018 |
| AE Motorworx Limited | Wholesale & retail | 2025 |
| Aero 21 Ltd. | Professional & scientific | 2003 |
| Aero Vista UAV Ltd | Professional & scientific | 2018 |
| AERO21 Property Ltd | Real estate | 2018 |
| Aetee Group Ltd | Manufacturing | 2026 |
| Aetee Limited | Wholesale & retail | 2001 |
| Affiliate Nexus Limited | Professional & scientific | 2025 |
| AG Gibbs (2021) Ltd | Public administration | 2021 |
| AG Landscaping Ltd | Administrative & support | 2022 |
| AGA Locum Services Ltd | Wholesale & retail | 2025 |
| Agile Incs Ltd | Professional & scientific | 2013 |
| Agitate Ltd | Professional & scientific | 2022 |
| AGN Estates Ltd | Real estate | 2022 |
| Agnes Real Estate Ltd | Real estate | 2023 |
| Agtech Media Ltd | Information & communication | 2023 |
| AI Capital Partners Limited | Real estate | 2022 |
| AI4HUMANS Limited | Professional & scientific | 2025 |
| AIM Asset Management Ltd | Financial services | 2018 |
| Aipad Limited | Wholesale & retail | 2024 |
| Airbnb - TOM Kirby Limited | Hospitality | 2025 |
| Aiwatch Limited | Wholesale & retail | 2024 |
| Ajipm Ltd | Construction | 2021 |
| AJP Amusements Limited | Arts & recreation | 2014 |
| AJP Electrical Distributors Limited | Wholesale & retail | 2011 |
| AJP Group Limited | Financial services | 2024 |
| AJT Plastering & Building Solutions Ltd | Construction | 2026 |
| AJW Land & Development Ltd | Administrative & support | 2020 |
| Akura Limited | Wholesale & retail | 1998 |
| Akute Limited | Professional & scientific | 2009 |
| Alan Walker (Consultancy & Training) Limited | Administrative & support | 2015 |
| Alcop Ltd | Other services | 2011 |
| Aldridge Electrical Installations Ltd | Construction | 2013 |
| Aldridge Jake Industries Limited | Wholesale & retail | 2026 |
| Algaepower Ltd | Energy | 2024 |
| ALH Eyecare Ltd | Wholesale & retail | 2022 |
| ALI Para Limited | Real estate | 2026 |
| Alien Technosignatures Research Group Ltd | Professional & scientific | 2023 |
| ALL Done and Dusted Cleaning Services Domestic Cotswolds Ltd | Administrative & support | 2019 |
| ALL in the Bottle Limited | Administrative & support | 2024 |
| ALL Natural Ways Ltd | Manufacturing | 2021 |
| ALL Seeing EYE Ltd. | Information & communication | 2014 |
| Allen & Carr Ltd | Administrative & support | 2011 |
| Allen Bros (Stroud) Ltd | Construction | 2023 |
| Allen's Plastering & General Building Ltd. | Construction | 2016 |
| Alliance for Camphill Ltd. | Health & social care | 2018 |
| Allied Art Ltd | Wholesale & retail | 2026 |
| Allpoints Dental Laboratory Limited | Professional & scientific | 2014 |
| Alpha Broadcast Facilities Limited | Arts & recreation | 2018 |
| Alpha-Tech Fire Limited | Manufacturing | 2011 |
| Alta Hagan Thorne Limited | Wholesale & retail | 2012 |
| Altrix Consultants Limited | Professional & scientific | 2002 |
| Aluminium Profile Engineering Limited | Manufacturing | 2015 |
| Alveston Wind Park Limited | Professional & scientific | 2007 |
| AM Building and Carpentry Ltd | Construction | 2018 |
| AMA Partners Ltd | Construction | 2024 |
| Amalfi Minchinhampton Limited | Hospitality | 2023 |
| Amari Designs Limited | Wholesale & retail | 2007 |
| Amaroo Solutions Limited | Wholesale & retail | 2018 |
| Amber Kinetic Ltd | Professional & scientific | 2024 |
| Amber Violins Ltd | Other services | 2011 |
| Amberheat (Stroud) Ltd | Construction | 2013 |
| Amberley Doors & Windows Ltd | Manufacturing | 2000 |
| Amberley Publishing Holdings Limited | Information & communication | 2005 |
| Amethyst Serviced Lets Ltd | Hospitality | 2016 |
| AML Property Holdings Limited | Real estate | 2021 |
| Amrida Limited | Real estate | 2026 |
| Anderson Architecture Limited | Professional & scientific | 2012 |
| Andrew Davidson Illustration Ltd | Professional & scientific | 2014 |
| Andrews Anaesthesia Ltd | Health & social care | 2019 |
| Andy Kane Ltd | Professional & scientific | 2014 |
| Angeli Idee Limited | Wholesale & retail | 2021 |
| Anglo Panda Limited | Education | 2019 |
| Anglo-Asia Investment Limited | Wholesale & retail | 2005 |
| Anna Booth Therapies Limited | Other services | 2018 |
| Anprcheq Limited | Wholesale & retail | 2018 |
| Antenna Music Limited | Arts & recreation | 2013 |
| Anthony Carter Limited | Administrative & support | 2003 |
| Antonia Wright Limited | Professional & scientific | 2020 |
| Antrobus Consulting Limited | Professional & scientific | 2017 |
| Anymore Ltd | Professional & scientific | 2023 |
| Anywork Anywhere Limited | Professional & scientific | 2000 |
| APE Barriers Limited | Manufacturing | 2021 |
| Apex Tracking Ltd | Transport & logistics | 2024 |
| App Consultancy Limited | Professional & scientific | 2014 |
| Apperley Holdings Ltd | Professional & scientific | 2024 |
| Applied Business Support Ltd | Administrative & support | 2018 |
| Applied Change Ltd | Professional & scientific | 2009 |
| Applied Knowledge and Technology Limited | Wholesale & retail | 2010 |
| Applied Science Solutions Ltd | Professional & scientific | 2023 |
| Aqua Oleum Limited | Manufacturing | 2015 |
| Aqua Services Ltd | Construction | 2002 |
| Aqua Stone Pool Finishes Ltd | Construction | 2006 |
| Arbour Clinic Limited | Health & social care | 2018 |
| Arcade Archive Ltd | Arts & recreation | 2022 |
| Arch Software Limited | Wholesale & retail | 1999 |
| Archenfield Properties Limited | Real estate | 2011 |
| Archetype Fine Art Services Limited | Arts & recreation | 2018 |
| Ardreco Ltd | Real estate | 2011 |