| RPB Flooring (Scotland) Limited | Construction | 2017 |
| Rrss Engineering Limited | Construction | 2015 |
| RS Electrics Scotland Ltd | Manufacturing | 2021 |
| Ryze Capital Limited | Financial services | 2024 |
| S a Boyd Services Ltd | Manufacturing | 2019 |
| SAL Strategic Ltd | Professional & scientific | 2016 |
| Sales I T a Limited | Transport & logistics | 2010 |
| SAS Rail Limited | Construction | 2020 |
| SC Learning Centre Ltd | Education | 2021 |
| Scotia Estates Ltd | Real estate | 2022 |
| Scotty Tours Limited | Transport & logistics | 2013 |
| Sggc Ltd | Construction | 2022 |
| Sharkaroos Ltd | Wholesale & retail | 2026 |
| Shoker Business Solutions Limited | Professional & scientific | 2026 |
| Showcase Space Photography Ltd | Professional & scientific | 2024 |
| Silver Birch Locuming Ltd | Professional & scientific | 2025 |
| Silver Screen Prop Guns Limited | Extraterritorial | 2023 |
| Sloan Russell Ltd | Administrative & support | 2025 |
| Sofaking ( Lanarkshire) Limited | Wholesale & retail | 2008 |
| SOK Construction Ltd | Construction | 2016 |
| Some Company (Scotland) Limited | Real estate | 2018 |
| South West Paint Supplies Limited | Administrative & support | 1949 |
| Specialized Glazing Services Limited | Construction | 2005 |
| Spirited Blether Ltd | Manufacturing | 2024 |
| Sportflight Scotland Limited | Education | 2006 |
| Springvale Wellbeing Ltd | Health & social care | 2023 |
| SRK UK Property Investments Ltd | Real estate | 2021 |
| Steyn Property Ltd | Other services | 2009 |
| Stobieside Consulting Ltd | Professional & scientific | 2004 |
| Stobieside Digital Engineering Limited | Professional & scientific | 2025 |
| Stormcloud Alpacas Limited | Agriculture & fishing | 2022 |
| Strathaven After School Club | Other services | 2000 |
| Strathaven Airfield Limited | Arts & recreation | 2015 |
| Strathaven Carpet and Upholstery Cleaning Ltd | Administrative & support | 2025 |
| Strathaven Castles Ltd | Administrative & support | 2021 |
| Strathaven Enterprise Ltd | Other services | 2025 |
| Strathaven Foot Clinic Limited | Health & social care | 2018 |
| Strathaven Golf Club (2022) Limited | Arts & recreation | 2022 |
| Strathaven Golf Club, Ltd. (the) | Arts & recreation | 1907 |
| Strathaven Model Society Limited | Other services | 1999 |
| Strathaven Rugby Football Club Ltd | Arts & recreation | 2016 |
| Strathaven Stars Theatre Group CIC | Arts & recreation | 2025 |
| Strathaven Town Mill Arts and Heritage Centre | Arts & recreation | 2009 |
| Strathclyde Collision Investigation Division Limited | Financial services | 2013 |
| Straven Construction Services Limited | Construction | 1980 |
| Studio Iapetus Ltd | Wholesale & retail | 2025 |
| Sunny Rentals Spain Ltd | Real estate | 2024 |
| Sweetie Brae Private Nursery Limited | Health & social care | 1996 |
| Synthesys Ltd | Information & communication | 2023 |
| Taal Property Limited | Real estate | 2022 |
| Taal Restaurant Carluke Limited | Hospitality | 2022 |
| Taal Restaurant Limited | Hospitality | 2016 |
| Telebadger Ltd | Wholesale & retail | 2025 |
| That's My Understanding Limited | Real estate | 2020 |
| THE Drawing Room Strathaven Limited | Hospitality | 2026 |
| THE Miniature Bottle Shop Ltd | Wholesale & retail | 2022 |
| THE Rissco Collection Limited | Hospitality | 2016 |
| THE Roof and Gutter Company Ltd. | Construction | 2012 |
| THE Scottish Flying Club | | 2006 |
| THE Sign Company (Glasgow) Ltd | Manufacturing | 2023 |
| THE Weavers (Strathaven ) Limited | Hospitality | 2021 |
| Thermal Image Scotland Limited | Professional & scientific | 2008 |
| Thermal Image UK Limited | Professional & scientific | 2008 |
| Thermalchill Ltd | Professional & scientific | 2025 |
| Thewinningteam Ltd | Administrative & support | 2024 |
| Think Scotland Limited | Extraterritorial | 2006 |
| Thistlerose Fitness Ltd | Arts & recreation | 2020 |
| Thornbridge Management Accountancy Ltd | Professional & scientific | 2014 |
| Three C'S Strathaven Limited | Real estate | 2025 |
| Threestanes Limited | Extraterritorial | 2010 |
| TIM Lever Distribution Ltd. | Administrative & support | 2008 |
| Timeless Taste Events Limited | Hospitality | 2025 |
| Top Nail Strathaven Ltd | Other services | 2024 |
| Total EV Ltd | Construction | 2023 |
| Total Housing Ltd | Real estate | 2024 |
| Townfoot Consulting Ltd | Professional & scientific | 2025 |
| Transitio Ltd | Administrative & support | 2022 |
| TSS Joinery Ltd | Construction | 2021 |
| Tudor Coffee House Ltd | Hospitality | 2025 |
| UP Front Brewing Limited | Manufacturing | 2015 |
| Upper Crust Associates Limited | Transport & logistics | 1980 |
| Urb@N Ltd | Extraterritorial | 2024 |
| V2 Drilling Tools Limited | Manufacturing | 2014 |
| Venice of the North Ltd | Information & communication | 2017 |
| Vieve Ltd | Wholesale & retail | 2019 |
| VKM & Family Ltd | Financial services | 2023 |
| Vlogify Ltd | Information & communication | 2018 |
| Vocagency Ltd | Professional & scientific | 2021 |
| VTV Ventures Ltd | Construction | 2021 |
| Vulcanus Technical Services Limited | Construction | 2017 |
| Wastesync Ltd | Information & communication | 2026 |
| Waterside Strathaven Limited | Hospitality | 2024 |
| Well Pict Limited | Professional & scientific | 2001 |
| West Browncastle Farm Ltd | Extraterritorial | 2017 |
| West of Scotland Window Company Limited | Real estate | 1988 |
| Wheeldon Clinical Services Limited | Health & social care | 2019 |
| Whiskywheels Limited | Administrative & support | 2018 |
| Whiteshawgate Investments Ltd | Real estate | 2024 |
| Wildcat Project Management Ltd | Professional & scientific | 2025 |
| William Shearer Livestock Ltd | Agriculture & fishing | 2026 |