| Dragon Equipment Ltd | Manufacturing | 2018 |
| Dragon Hoard Ltd | Wholesale & retail | 2025 |
| Dragon's View (Management Company) Limited | Households | 2023 |
| DRE Electrical (Anglia) Limited | Construction | 2020 |
| Drew Develops Ltd | Real estate | 2024 |
| Drew Flips Ltd | Real estate | 2024 |
| Drinsey Farming Company Ltd | Agriculture & fishing | 2021 |
| Droneops Ltd | Information & communication | 2023 |
| DS3 Ltd | Construction | 2020 |
| DSC Training & Assessments Limited | Professional & scientific | 2022 |
| Dsmi Limited | Other services | 2019 |
| DSN Synergy Health Ltd | Health & social care | 2020 |
| DSN Synergy Properties Limited | Real estate | 2020 |
| Dstreme Limited | Professional & scientific | 2023 |
| Dstrsd Desires Ltd | Wholesale & retail | 2025 |
| Dunlop Web Limited | Information & communication | 2010 |
| Durrants Plumbing & Heating Limited | Construction | 2017 |
| DVP Surgical Referrals Limited | Professional & scientific | 2022 |
| Dwell Online Estates Ltd | Real estate | 2015 |
| Dynamic Coach Ltd | Professional & scientific | 2017 |
| Dyson & Mortar Limited | Real estate | 2017 |
| Dyson & Robinson Limited | Real estate | 2017 |
| E & D Boiler Maintenance Limited | Construction | 2011 |
| E R Construction Services Limited | Professional & scientific | 2019 |
| E V East Limited | Wholesale & retail | 2024 |
| E3 Cleaning Services Ltd | Administrative & support | 2025 |
| Earl Soham Joinery Limited | Manufacturing | 2022 |
| Early Bird Farmads Limited | Other services | 2021 |
| Early Bird Farming Publications Limited | Information & communication | 1979 |
| Early Bird Fruit Publications Ltd | Administrative & support | 2020 |
| Early Bird Properties (East Anglia) Limited | Real estate | 2023 |
| East Anglia Locums Limited | Administrative & support | 2023 |
| East Anglia Services (Freston) Limited | Construction | 2018 |
| East Anglian Property Services Limited | Real estate | 2024 |
| East Coast Adventures ECA Limited | Arts & recreation | 2023 |
| East Harbour Property Ltd | Real estate | 2018 |
| East of England Spine Limited | Health & social care | 2012 |
| Eastern Arboriculture Training Ltd | Education | 2022 |
| Eastern Concrete Limited | Manufacturing | 2002 |
| Eastern Property Rentals Limited | Hospitality | 2023 |
| Eastern Sports Limited | Professional & scientific | 2019 |
| Eastrada Facilities Limited | Administrative & support | 2025 |
| Eastrada Limited | Construction | 2020 |
| Easyestimate.Co.Uk Ltd | Information & communication | 2016 |
| Eaton Hill Advisory SPV Limited | Information & communication | 2017 |
| Eazy Gardening Direct Ltd | Wholesale & retail | 2024 |
| Eazy Gardening Ltd | Wholesale & retail | 2019 |
| Ebbnflow Investment Properties Limited | Households | 2026 |
| EC1 Education Consultants Limited | Education | 2025 |
| ECR Properties Limited | Real estate | 2013 |
| Edge Medical Aesthetics Ltd | Health & social care | 2025 |
| Edintheclouds Ltd | Information & communication | 2018 |
| EFM Stowmarket Limited | Households | 2011 |
| Egaa Limited | Real estate | 2019 |
| EJC People Solutions Limited | Professional & scientific | 2019 |
| EJR Ceramics Limited | Wholesale & retail | 2019 |
| Electrical Investigation Ltd | Professional & scientific | 2020 |
| Electricians Ipswich Ltd | Construction | 2018 |
| Eliboll Limited | Hospitality | 2024 |
| Eliotte Limited | Professional & scientific | 2006 |
| Elite Automotive Leather Ltd | Wholesale & retail | 2020 |
| Elite Film Works Limited | Professional & scientific | 2019 |
| Elite Spraybooth Services Limited | Manufacturing | 2020 |
| Elizabeth Talbot Limited | Professional & scientific | 2019 |
| ELM Occasions Ltd | Manufacturing | 2011 |
| Elmjoy Productions Limited | Information & communication | 2011 |
| Elmswell Contractors 2006 Limited | Professional & scientific | 2006 |
| Elmswell Contractors Limited | Households | 1982 |
| Elmtree Property Management Limited | Real estate | 2006 |
| Elmwood Safety Solutions Ltd | Professional & scientific | 2022 |
| ELS & Co Enterprises Ltd | Wholesale & retail | 2019 |
| Elvance Ltd | Information & communication | 2022 |
| Elwent Building Services Limited | Construction | 2019 |
| Elwood Landscape Design Limited | Professional & scientific | 2006 |
| Emberleaf Property Limited | Real estate | 2026 |
| Emerald Aesthetics 23 Limited | Other services | 2023 |
| Emergence Property Limited | Real estate | 2018 |
| Emma Bradford & Co Mediation Limited | Professional & scientific | 2021 |
| Emma Plant Hire Ltd | Construction | 2020 |
| Emma Stanyer Consulting Limited | Professional & scientific | 2018 |
| Emma Thwaite Consulting Limited | Professional & scientific | 2013 |
| Emmy Consulting Limited | Professional & scientific | 2023 |
| Energised Electrons Limited | Professional & scientific | 2010 |
| Entastic Ltd | Extraterritorial | 2024 |
| Entirely British Ltd | Manufacturing | 2013 |
| Environmental Efficiency Limited | Professional & scientific | 2004 |
| Enviropartner UK Limited | Professional & scientific | 2016 |
| Epicagri Ltd | Wholesale & retail | 2022 |
| Epoc Innovations Ltd | Information & communication | 2023 |
| Equity Hive Limited | Real estate | 2021 |
| Errantco Limited | Construction | 2013 |
| Essex and Suffolk Rivers Trust | Professional & scientific | 2014 |
| Ethan Elliston-Hart (Cambridge) Limited | Wholesale & retail | 2024 |
| ETR H&S Services Limited | Professional & scientific | 2015 |
| Euro Bridge Advisory Ltd | Professional & scientific | 2025 |
| Euro Tool Depot Limited | Wholesale & retail | 2007 |
| European Gate Valves Limited | Manufacturing | 1998 |
| Evans Homebuyers Ltd | Real estate | 2024 |
| Evans Sourcing Ltd | Real estate | 2020 |
| Evans Stays Ltd | Real estate | 2025 |