| Twenty Eight Investment Group Ltd | Financial services | 2024 |
| Twenty Four Investments Limited | Financial services | 2018 |
| Twenty/Twenty Engagement Limited | Professional & scientific | 2017 |
| Twin Clayton & Co Ltd | Manufacturing | 2020 |
| Twin Oaks Limited | Construction | 1988 |
| Two Thirsty Brewers Limited | Hospitality | 2012 |
| Twoje Biuro Fuzja Ltd | Other services | 2024 |
| TY Eaton Ltd | Real estate | 2021 |
| Tyler and Hassan Co Ltd | Hospitality | 2025 |
| Typewriter & Equipment Company Limited | Wholesale & retail | 1959 |
| U-Can (UN) Limited | Professional & scientific | 2003 |
| U3 Consultancy Ltd | Education | 2011 |
| UE Motors Ltd | Wholesale & retail | 2023 |
| Ugly Enough Limited | Wholesale & retail | 2021 |
| UH Mechanical and MOT Services Ltd | Wholesale & retail | 2022 |
| Uhealthcare Limited | Wholesale & retail | 2011 |
| UK Holdings FM Ltd | Financial services | 2024 |
| UK Lifting Store Limited | Wholesale & retail | 2018 |
| UK Office Furniture Limited | Wholesale & retail | 2022 |
| UK Property Stagers Ltd | Construction | 2022 |
| UK Rapid Logistics Ltd | Transport & logistics | 2020 |
| UK Traders and Investment Limited | Financial services | 2013 |
| Ultima Tours Holdings Limited | Financial services | 2018 |
| Ultima Tours Limited | Administrative & support | 1989 |
| UM Storage Systems Ltd | Construction | 2023 |
| Unbioctium Limited | Financial services | 2024 |
| Ungurul Ltd | Transport & logistics | 2025 |
| Unhinged Positivity Ltd | Information & communication | 2026 |
| UNI Accessories Limited | Wholesale & retail | 2017 |
| Uniled Limited | Manufacturing | 2025 |
| Uniqueat Ltd | Hospitality | 2016 |
| Unisant Limited | Real estate | 1970 |
| Unisant Midlands Limited | Professional & scientific | 1972 |
| Unit 2 Branding & Collectables Ltd | Wholesale & retail | 2026 |
| United Counties Office Equipment Limited | Wholesale & retail | 1965 |
| Universal Auto Hub Ltd | Wholesale & retail | 2025 |
| Universal Laws Trading Ltd | Wholesale & retail | 2019 |
| Universal Steel Profiles Limited | Manufacturing | 2009 |
| Upfront Entertainments Agency Limited | Other services | 2000 |
| Uplandhouse Ltd | Construction | 2025 |
| Uproar Social Media Ltd | Professional & scientific | 2023 |
| Upside Records Ltd | Information & communication | 2005 |
| Upwise Limited | Real estate | 1994 |
| Urban Prospects MMA Limited | Arts & recreation | 2024 |
| Urzica Build and Solve Limited | Construction | 2024 |
| Utilities Broadway Ltd | Construction | 2024 |
| UVH Management Ltd | Extraterritorial | 2019 |
| V & V Solutions Limited | Information & communication | 2004 |
| V and KB Ltd | Arts & recreation | 2025 |
| V&S Happy Bees Limited | Wholesale & retail | 2017 |
| V.Op.Audit and Management Limited | Professional & scientific | 2026 |
| V1 Precision Engineering Limited | Manufacturing | 2015 |
| Vacuumatic Machines UK Ltd | Manufacturing | 2023 |
| Vadk Developments Ltd | Construction | 2021 |
| Valet Touch Ltd | Administrative & support | 2024 |
| Vallets Farm Barn Alpacas Ltd | Other services | 2023 |
| Van Monkey Limited | Wholesale & retail | 2010 |
| Vanguard 2024 Limited | Manufacturing | 2024 |
| Vanguard Foundry Limited | Manufacturing | 1983 |
| Vanity Beauty & Hair Ltd | Other services | 2024 |
| Vanoova Ltd | Transport & logistics | 2025 |
| Vantage Education Ltd | Education | 2011 |
| Vapeorium Ltd | Wholesale & retail | 2025 |
| Varialift Airships PLC | Manufacturing | 2007 |
| Variaplan Limited | Manufacturing | 2014 |
| Varidium Limited | Information & communication | 2002 |
| VAS MAS Salon and Style Limited | Other services | 2025 |
| Vatafu Construct Limited | Construction | 2026 |
| Vaughans (Hope Works) Limited | Manufacturing | 2017 |
| Vehicle Security Solutions Limited | Wholesale & retail | 2016 |
| Vehiclefit Ltd | Wholesale & retail | 2013 |
| Venator Investigations Ltd | Administrative & support | 2024 |
| Vented Engineering Services Ltd | Construction | 2024 |
| Venture Lifestyles Ltd | Wholesale & retail | 2020 |
| Venture Technology and Services Ltd | Information & communication | 2007 |
| Veralta Limited | Education | 2026 |
| Vernons Plant Hire Ltd | Construction | 2005 |
| Verrex Ltd | Wholesale & retail | 2008 |
| Versa Group Limited | Construction | 2017 |
| Vertex Composite Ltd | Wholesale & retail | 2026 |
| Vestan Ltd | Arts & recreation | 2023 |
| VIA IT Services Limited | Information & communication | 2003 |
| VIABLE2BUYABLE Limited | Professional & scientific | 2024 |
| Viaduct Service Clent Ltd | Construction | 2019 |
| Viaduct Stourbridge Limited | Wholesale & retail | 2015 |
| Viba Private Ltd | Hospitality | 2026 |
| Vibe (Atwal) Limited | Wholesale & retail | 2009 |
| Vicarage Nursing Home Limited | Health & social care | 2003 |
| Vicars Coppice Property Development Ltd | Real estate | 2018 |
| Vickers Aeromarine Limited | Construction | 2016 |
| Victoria Whitchurch Limited | Professional & scientific | 2014 |
| Viermont Limited | Information & communication | 2003 |
| Vilais (Twister) LLP | | 2007 |
| Village Views Management Company Limited | Households | 2009 |
| Vineary Properties Limited | Households | 2017 |
| Virgo Projects Ltd | Information & communication | 2011 |
| Virtual Office Solutions Limited | Administrative & support | 2011 |
| Virusxap Limited | Administrative & support | 2020 |
| Vision Division Community Interest Company | Extraterritorial | 2014 |
| Vision Fire Safety Solutions Limited | Construction | 2019 |