| Higher Stockbridge Ltd. | Energy | 2018 |
| Hogg & Smith Ltd | Construction | 2013 |
| Hollier Engineering Limited | Information & communication | 2005 |
| Home Concierge Service Limited | Administrative & support | 2022 |
| Home-Start Stroud and Gloucester | Health & social care | 2004 |
| Homer Properties Limited | Real estate | 2004 |
| Hope for Tomorrow | Health & social care | 2002 |
| Hopelands Preparatory School | Education | 1991 |
| Hot Performance Media Limited | Professional & scientific | 2025 |
| Howletts Honeybees Ltd | Agriculture & fishing | 2015 |
| HP Air Systems Limited | Professional & scientific | 2013 |
| HRS Dentalcare Limited | Health & social care | 2011 |
| Hutchins & SON Electrical Ltd | Construction | 2026 |
| I Scream Ice Cream Ltd | Manufacturing | 2017 |
| I.T Security Plus Limited | Information & communication | 2006 |
| IAN Bartlett Taping Ltd | Construction | 2014 |
| IAN Rice Building Limited | Construction | 2012 |
| IB Electrics (Glos) Ltd | Construction | 2014 |
| Icosa Water Limited | Water & waste | 2014 |
| IHR Ideal Home Range U.K. Ltd. | Wholesale & retail | 1996 |
| Imas Group (UK) C.I.C. | Health & social care | 2023 |
| Immersa Developments Limited | Health & social care | 2024 |
| Immersa Large Scale Limited | Professional & scientific | 2024 |
| IN EAR Entertainment Limited | Information & communication | 2010 |
| In-Map Limited | Wholesale & retail | 1995 |
| Incotechnic Property Holdings Limited | Real estate | 2026 |
| Infinity 81 Limited | Transport & logistics | 2016 |
| Innovateinary MED Limited | Administrative & support | 2022 |
| Inntegra Ltd | Hospitality | 2015 |
| Insight & Perspective Ltd | Information & communication | 2015 |
| Insightfuse Digital Ltd | Information & communication | 2026 |
| Insite UK Holdings Limited | Professional & scientific | 2021 |
| Inspire Designs (UK) Ltd | Professional & scientific | 2007 |
| Install Construct Limited | Construction | 2018 |
| Intelligent (UK Holdings) Limited | Administrative & support | 2008 |
| Intelligent Agriculture Limited | Agriculture & fishing | 2009 |
| Intelligent Armour Limited | Wholesale & retail | 2008 |
| Intelligent Canine Limited | Information & communication | 2010 |
| Intelligent Equine Limited | Information & communication | 2010 |
| Intelligent Protection International Ltd | Administrative & support | 2009 |
| Intelligent Risk Analysis Limited | Professional & scientific | 2023 |
| Intelligent Training International Ltd | Administrative & support | 2008 |
| International Intelligence Limited | Administrative & support | 2002 |
| International Panel Products Limited | Wholesale & retail | 1987 |
| International Plywood (Aviation) Limited | Transport & logistics | 2013 |
| International Plywood (Gloucester) Ltd | Administrative & support | 1987 |
| International Plywood (Importers) Limited | Wholesale & retail | 1981 |
| International Plywood PLC | Financial services | 1981 |
| Investdev Ltd | Professional & scientific | 2017 |
| Irenewables Limited | Information & communication | 2003 |
| Ironcrafts (Stroud) Ltd. | Manufacturing | 2002 |
| ISN Partners Limited | Information & communication | 2014 |
| Itamin Ltd | Information & communication | 2021 |
| Itstick Labels Limited | Wholesale & retail | 1999 |
| J Etherton Building Conservation Ltd | Construction | 2009 |
| J&M Building & Maintenance Services (Gloucester) Ltd | Construction | 2025 |
| J&N Landscaping Ltd | Administrative & support | 2024 |
| J.E. Hooper Limited | Wholesale & retail | 1955 |
| J.P.U. Services Limited | Administrative & support | 2002 |
| J.T.C. Electrical Limited | Administrative & support | 2005 |
| Jackie Mcweeney Interiors Limited | Professional & scientific | 2017 |
| Jagged Edge Design Limited | Information & communication | 2003 |
| Jake Properties Limited | Real estate | 2025 |
| Jamats Academy Limited | Other services | 2018 |
| James Bishop Limited | Professional & scientific | 2014 |
| Jamie Bruce Limited | Professional & scientific | 2025 |
| Jane Maitland Consulting Limited | Professional & scientific | 2014 |
| JAS Innovative Limited | Administrative & support | 2014 |
| Jasmine FIC Ltd | Real estate | 2023 |
| Jeffery Smith Construction Management Limited | Construction | 2007 |
| Jenkins Swim Studio Ltd | Education | 2025 |
| Jeti Limited | Professional & scientific | 2022 |
| Jewkes Ltd | Financial services | 2026 |
| JJS 1 Limited | Energy | 2023 |
| JJS 2 Limited | Energy | 2023 |
| JJS 3 Limited | Energy | 2023 |
| Jklh Properties Limited | Real estate | 2024 |
| JLS Trading Limited | Wholesale & retail | 2016 |
| JMH Contractors Limited | Construction | 2024 |
| JMW Financial Services Limited | Financial services | 2021 |
| JOF Limited | Education | 2024 |
| John Kerry & Sons Limited | Wholesale & retail | 2008 |
| JP Investco 1 Limited | Professional & scientific | 2020 |
| JP Investigation Services Ltd | Professional & scientific | 2021 |
| JPR Environmental Ltd | Construction | 2002 |
| JPU Construct Ltd | Construction | 2026 |
| JRC Holdings (Stonehouse) Limited | Financial services | 2026 |
| JRL Associates Ltd | Administrative & support | 2014 |
| JS Accountancy Services Limited | Professional & scientific | 2002 |
| JSC Amusements Ltd | Arts & recreation | 2017 |
| JSE Group Services Ltd | Construction | 2020 |
| Jssm Properties Limited | Extraterritorial | 2025 |
| Judd Martin Limited | Construction | 2014 |
| Justice Management Limited | Information & communication | 1994 |
| Justin Davidson Limited | Health & social care | 2012 |
| JW Architectural Studio Ltd | Professional & scientific | 2025 |
| K.Holm Inn Ltd | Hospitality | 2021 |
| Kaff Shoes Services Ltd | Wholesale & retail | 2024 |
| Kalera Properties Ltd | Construction | 2025 |
| Kane Property Ltd | Real estate | 2026 |