| Dynamicminds Recruitment Ltd | Administrative & support | 2023 |
| E E S I Limited | Construction | 1994 |
| E Partridge Building Services Limited | Construction | 2014 |
| E Partridge Properties Limited | Real estate | 2024 |
| Earthcott Battery Storage Limited | Energy | 2022 |
| Eastcombe Stores Ltd | Wholesale & retail | 2012 |
| Eastgate Solar Ltd | Energy | 2021 |
| Eastington Farm Shop Limited | Hospitality | 2025 |
| Ebley Court Ltd | Real estate | 2023 |
| Ebley Estates Limited | Construction | 2020 |
| EDM Solutions (Stroud) Limited | Manufacturing | 2012 |
| Ed’S Fitness Ltd | Arts & recreation | 2024 |
| Eesibuild Ltd | Construction | 2014 |
| Effimove UK Limited | Information & communication | 2022 |
| Eggleton Communications Limited | Professional & scientific | 2016 |
| Eish Ltd | Wholesale & retail | 2010 |
| EJB Therapy Ltd | Health & social care | 2014 |
| Ekoflow Limited | Water & waste | 2018 |
| Electrical & Electronic System Installations Limited | Extraterritorial | 2004 |
| Eleighmentary Group Ltd | Professional & scientific | 2016 |
| Elementa Leadership Limited | Professional & scientific | 1999 |
| Elevenci Limited | Professional & scientific | 2019 |
| Elite Electrical Ltd | Construction | 2015 |
| Elite Sports Physiotherapy Ltd | Health & social care | 2012 |
| Ellis Transport Limited | Transport & logistics | 1994 |
| Elmrep Limited | Extraterritorial | 1984 |
| Emerald Training & Development Limited | Professional & scientific | 2002 |
| Emma Owen Limited | Administrative & support | 2023 |
| Empathy Rooms Limited | Health & social care | 2015 |
| Emstra Limited | Construction | 2012 |
| END of Line Fabrics Limited | Wholesale & retail | 2012 |
| Energy Technology Limited | Mining & quarrying | 2024 |
| EPIC1 Management Limited | Real estate | 2020 |
| Epilog Consulting Limited | Professional & scientific | 2023 |
| EPS (Bristol) Ltd | Construction | 2015 |
| EQ Gleam Limited | Wholesale & retail | 2025 |
| Equiboodle Limited | Wholesale & retail | 2011 |
| Erfa Limited | Professional & scientific | 2012 |
| Eric Cole Ltd | Professional & scientific | 2007 |
| Escapism Entertainment Ltd | Information & communication | 2016 |
| Escart Windfarm Ltd | Energy | 2025 |
| Ethos Electrical Limited | Manufacturing | 2018 |
| Eurodental Limited | Real estate | 2003 |
| European Blues Association | Education | 2000 |
| Evac Medical Limited | Manufacturing | 2018 |
| EVE Connect Ltd | Information & communication | 2020 |
| EVE Networks Group Ltd | Information & communication | 2020 |
| Event Property Holdings Limited | Real estate | 2025 |
| Everglow Beauty Care Ltd | Wholesale & retail | 2024 |
| Evoluto Limited | Information & communication | 2020 |
| Evolve Care Consultancy Ltd | Professional & scientific | 2026 |
| Executive Development International Limited | Professional & scientific | 1999 |
| Exled Golf Ltd | Other services | 2023 |
| Exled Limited | Manufacturing | 2004 |
| Expansion Games Limited | Information & communication | 2019 |
| Eyes in the Sky Limited | Administrative & support | 2016 |
| F2P Holdings Ltd | Information & communication | 2018 |
| Fabs Global Foods Ltd | Wholesale & retail | 2026 |
| Fairview Bess Limited | Energy | 2022 |
| Feckenham Battery Storage Limited | Energy | 2022 |
| Feel Complete Group Ltd | Information & communication | 2022 |
| Fever Property Ltd | Real estate | 2009 |
| FG Ottico Limited | Professional & scientific | 2010 |
| Fine Pines Corporate Limited | Wholesale & retail | 2018 |
| Firerelish Ltd | Professional & scientific | 2025 |
| First Day Limited | Information & communication | 1998 |
| Five Trees Furniture Limited | Wholesale & retail | 2020 |
| Five Valleys Business Finance Ltd | Professional & scientific | 2021 |
| Five Valleys Digital Ltd | Information & communication | 2026 |
| Five Valleys Hamper Co Limited | Wholesale & retail | 2025 |
| Five Valleys Labels Ltd | Manufacturing | 1998 |
| Fjords Processing Limited | Professional & scientific | 1993 |
| Flaxlands Kennels Limited | Agriculture & fishing | 2007 |
| Fleetcheck Limited | Administrative & support | 2006 |
| Fleetfind Limited | Administrative & support | 2002 |
| Fleur Bailey Nutrition Ltd | Health & social care | 2019 |
| Flexed Limited | Professional & scientific | 2006 |
| Flip and Twist Gymnastics Community Interest Company | Arts & recreation | 2013 |
| Flourish (Gloucestershire) Limited | Health & social care | 2016 |
| Flourish Care Holdings Limited | Health & social care | 2016 |
| Flourish Design Consultancy Ltd | Professional & scientific | 2013 |
| Flying Monk Graphics Ltd | Other services | 2005 |
| Foamtech Antifire Ltd | Public administration | 2018 |
| Focus Interiors Limited | Construction | 1993 |
| Follett Accounts & Tax Limited | Professional & scientific | 2022 |
| Folly Fields Limited | Agriculture & fishing | 2016 |
| Food and Farming Compliance Ltd | Agriculture & fishing | 2011 |
| Footsure Western Limited | Wholesale & retail | 1980 |
| Fort Projects Limited | Information & communication | 2001 |
| Four Shires Financial Solutions Limited | Professional & scientific | 2011 |
| Foxglove Holdings Limited | Financial services | 2021 |
| Frame 2 Fame Limited | Information & communication | 2026 |
| Fraserburgh Battery Storage Limited | Administrative & support | 2018 |
| Frocester Consulting Limited | Information & communication | 2016 |
| Frocester Cricket Club Limited | Arts & recreation | 2018 |
| Frocester Cricket Club Supporters Group Limited | Arts & recreation | 2018 |
| Furlong Flooring (Wales) Ltd. | Wholesale & retail | 1974 |
| Fusion Digital Media Limited | Administrative & support | 2015 |
| Fusion Five Limited | Extraterritorial | 2004 |
| FWR Estates Limited | Real estate | 2020 |