| Antigenika Ltd | Professional & scientific | 2024 |
| Bhse Chilterns Stokenchurch Management Company Limited | Real estate | 2006 |
| BJA Trading Holdings Limited | Professional & scientific | 2024 |
| Bryant Limited | Wholesale & retail | 1972 |
| Buddy BOY Productions Ltd | Arts & recreation | 2025 |
| Camilla Gordon Ltd | Professional & scientific | 2022 |
| Coppice Gardens Management Co Ltd | Households | 2021 |
| D & R Plumbing & Heating Engineers Ltd | Construction | 2014 |
| D&M Automotive Group Ltd | Wholesale & retail | 2026 |
| DPM Limited | Administrative & support | 2009 |
| Everyday Specialty Drinks Ltd | Manufacturing | 2025 |
| Freer Financial Planning Ltd | Professional & scientific | 2021 |
| Gaia Care Homes Ltd | Health & social care | 2025 |
| Garsington Opera Enterprises Limited | Wholesale & retail | 2013 |
| Garsington Opera Limited | Arts & recreation | 1990 |
| George Road Management Company Limited | Households | 1984 |
| GK Rotary Limited | Professional & scientific | 2022 |
| Gwalior Sweets Limited | Hospitality | 2025 |
| HM SVS Limited | Wholesale & retail | 2019 |
| Home Based Salons UK Ltd | Manufacturing | 2024 |
| Home Plastering Services Ltd | Construction | 2021 |
| Inpharmation Limited | Information & communication | 1998 |
| JSL Services Group Limited | Information & communication | 2015 |
| MCB Store Ltd | Wholesale & retail | 2024 |
| MFM Properties Limited | Real estate | 2022 |
| Nico Construct Ltd | Construction | 2021 |
| Perfectly Precured Ltd | Wholesale & retail | 2017 |
| Quadranet Systems Limited | Information & communication | 2002 |
| Rail Logistics Consultancy Ltd | Water & waste | 2016 |
| Roseash Limited | Wholesale & retail | 1994 |
| Spamco Ltd | Professional & scientific | 1992 |
| Specialist Paint and Maintenance Chemical Company Limited | Wholesale & retail | 1981 |
| Starpeak Insurance Solutions Limited | Financial services | 1991 |
| Swilley Gardens RTM Company Limited | Households | 2025 |
| Test and Inspect Safety Services Ltd | Construction | 2023 |
| THE Heights Stokenchurch Freehold Limited | Real estate | 2017 |
| THE Kennel Shop Limited | Wholesale & retail | 2018 |
| THE Old Post Solutions Ltd | Professional & scientific | 2011 |
| THE Print Shop Limited | Wholesale & retail | 2002 |
| Treefield Distribution Limited | Wholesale & retail | 2025 |
| Trkdout Studios Ltd | Information & communication | 2025 |
| Unthink Ltd | Professional & scientific | 2020 |