| First Called Limited | Wholesale & retail | 2020 |
| First Choice Autocentre Ltd | Manufacturing | 2009 |
| First Choice Building Plastics Ltd | Wholesale & retail | 2021 |
| First Choice Office Design Limited | Manufacturing | 2016 |
| First Contracts and Travel Limited | Transport & logistics | 2017 |
| First GAS Limited | Construction | 2021 |
| First Properties Limited | Construction | 1991 |
| First Property Services UK Limited | Administrative & support | 2009 |
| First Property Surveys Ltd | Administrative & support | 2019 |
| First Step Designs Holdings Ltd | Financial services | 2022 |
| First Step Designs Limited | Wholesale & retail | 2014 |
| First Step French Polishing Limited | Manufacturing | 2021 |
| First Technology Consultants Limited | Information & communication | 2010 |
| FIRST4CARE24(UK) Ltd | Health & social care | 2018 |
| FIRST4CHILDREN Education Ltd | Education | 2025 |
| FIRST4PAINTING and Decorating Ltd | Construction | 2024 |
| Firstcalluk Limited | Administrative & support | 2024 |
| Firstcapital (UK) Trading Limited | Real estate | 2013 |
| Firststep Properties Ltd | Real estate | 2025 |
| Firthwell Developments Limited | Construction | 2021 |
| Fish Lounge Longton Ltd | Hospitality | 2023 |
| Fishing the Past CIC | Education | 2025 |
| Fishkar Fishing Ltd | Wholesale & retail | 2021 |
| Fishtech Consulting Ltd | Information & communication | 2024 |
| Fiskars UK (Vita) Limited | Wholesale & retail | 2009 |
| Fission Recruitment Services Limited | Administrative & support | 2007 |
| Fit Females UK CIC | Arts & recreation | 2025 |
| Fit Quote Ltd | Wholesale & retail | 2011 |
| Fitflick Ltd | Information & communication | 2026 |
| Fitzjohn Building and Interiors Ltd | Construction | 2021 |
| Fitzjohn Pepper Properties Ltd | Real estate | 2023 |
| Fitzpatrick Properties Limited | Real estate | 2019 |
| Five H Investments Ltd | Real estate | 2015 |
| Five Oaks Property Management Limited | Real estate | 2022 |
| Five Towns JU Jitsu RYU Community Interest Company | Education | 2023 |
| Five Towns Stoke Ltd | Real estate | 2011 |
| FIX Recruitment Ltd | Administrative & support | 2025 |
| Fixit Laptops Ltd | Manufacturing | 2011 |
| FK Tyres Limited | Wholesale & retail | 2024 |
| Fkconsulting Ltd | Manufacturing | 2026 |
| Fkperioperativeservices Ltd | Health & social care | 2025 |
| FL Scaffold Limited | Construction | 2011 |
| Flame GAS Services Staffordshire Limited | Construction | 2015 |
| Flames Smallthorne Limited | Hospitality | 2024 |
| Flames Stokes Limited | Hospitality | 2024 |
| Flaming Grill Fast Food Ltd | Hospitality | 2025 |
| Flanders Fallen Stock Limited | Agriculture & fishing | 2018 |
| Flash Recovery’S and Repairs Ltd | Wholesale & retail | 2025 |
| Flat Lemonade Limited | Wholesale & retail | 2025 |
| Flat OUT Films Ltd | Information & communication | 2025 |
| Flavo Consultants Ltd | Construction | 2020 |
| Flavorful Fusion Ltd | Hospitality | 2026 |
| Flavourella Limited | Manufacturing | 2026 |
| Flavourz Forsbrook Ltd | Hospitality | 2024 |
| Flawless Fitters Ltd | Construction | 2025 |
| Flawless Venue Dressing Ltd | Professional & scientific | 2021 |
| Flawless Visage Ltd | Other services | 2024 |
| Fledgling Education CIC | Education | 2025 |
| Fleet Masters Stoke Limited | Administrative & support | 2026 |
| Fleetbridge Transport Ltd | Transport & logistics | 2026 |
| Fleetlink Transport Ltd | Transport & logistics | 2026 |
| Fletcher Bickerton Holdings Limited | Professional & scientific | 2008 |
| Fletcher Medical Reports Ltd | Other services | 2013 |
| Fletcher Scaffolding Limited | Construction | 2016 |
| Fleur Homes Limited | Real estate | 2018 |
| Fleur Property Developments Limited | Construction | 2016 |
| Fleur Property Lettings Limited | Real estate | 2017 |
| Flexible Vehicle Logistics (Stoke) Limited | Transport & logistics | 2023 |
| Fleximart Limited | Wholesale & retail | 2025 |
| Flexistaff Ltd. | Administrative & support | 2025 |
| Flic Flac Limited | Arts & recreation | 2004 |
| Flight Center Group Ltd | Wholesale & retail | 2026 |
| Fliq Limited | Information & communication | 2024 |
| FLO Studio Ventures Ltd | Information & communication | 2026 |
| FLO Utilities Limited | Construction | 2024 |
| Floare DE Colt Community Interest Company | Education | 2025 |
| Floor IT (Stoke) Limited | Wholesale & retail | 2017 |
| Floor Store Staffordshire Ltd | Construction | 2026 |
| Flopharm Ltd | Professional & scientific | 2025 |
| Floral Couture by Myke Limited | Wholesale & retail | 2025 |
| Florali Garden and Landscape Ltd | Construction | 2023 |
| Florence House (Staffordshire) Limited | Health & social care | 2011 |
| Florida Patches Ltd | Wholesale & retail | 2026 |
| Florin Concrete Floors Ltd | Construction | 2021 |
| Florin Lazar Ltd | Transport & logistics | 2018 |
| Florist Wedding & Craft Supplies Limited | Wholesale & retail | 2015 |
| Flour & Co Bakehouse Limited | Manufacturing | 2020 |
| Flourish Haven Limited | Wholesale & retail | 2024 |
| Flourish Montessori Day Nursery Ltd | Education | 2025 |
| Flower Fairy Ltd | Wholesale & retail | 2023 |
| Flowers on the Moon Limited | Professional & scientific | 2025 |
| Flowpilotuk Ltd | Administrative & support | 2026 |
| Fluffy Pancake Co Ltd | Hospitality | 2025 |
| Flux Design and Innovation Limited | Professional & scientific | 2021 |
| FLY Reagle Limited | Transport & logistics | 2021 |
| Flying Eagle Couriers Ltd | Transport & logistics | 2021 |
| Flysimtech Ltd | Information & communication | 2023 |
| Flytonic Services Ltd | Construction | 2020 |
| FM Media & Marketing Ltd | Administrative & support | 2021 |
| FMC24 Holdings Limited | Financial services | 2018 |