| Dt.Offshore.Engineering Limited | Professional & scientific | 2023 |
| DTB Drains Ltd | Construction | 2024 |
| DTH Consulting Ltd | Professional & scientific | 2018 |
| Duciyke Limited | Health & social care | 2025 |
| Dulce Skin Ltd | Health & social care | 2024 |
| Dulcie Property Co. Ltd | Real estate | 2025 |
| Duncan House (PP) Limited | Real estate | 2023 |
| Dunelm Court Management Company Limited | Households | 1994 |
| Dunmore Trading Ltd | Wholesale & retail | 2025 |
| Dunnell Estates Ltd | Real estate | 2020 |
| Dunotta Limited | Extraterritorial | 2006 |
| DUO Digital Marketing LLP | | 2021 |
| DUO Therm Limited | Construction | 2011 |
| Durabound Ltd | Wholesale & retail | 2014 |
| Durham Boathouse Limited | Other services | 2014 |
| Durham Digital Limited | Information & communication | 2020 |
| Durham Hire Limited | Construction | 2015 |
| Durham Korean Church | Other services | 2010 |
| Durham Lane Garage Ltd | Wholesale & retail | 2013 |
| Durham LOG Burners Limited | Construction | 2019 |
| Durham Scientific Crystals Limited | Professional & scientific | 2007 |
| Durhams Transport Training Ltd | Education | 2014 |
| DW Electrical (NE) Ltd | Construction | 2023 |
| Dwellings and Settings Ltd | Real estate | 2025 |
| DXN Commercial Property Limited | Real estate | 2022 |
| DXN Properties Ltd | Real estate | 2020 |
| Dxprpl Ltd | Construction | 2022 |
| DY Maintenance Ltd | Construction | 2025 |
| Dylan Abraham Logistics Ltd | Transport & logistics | 2025 |
| Dynamic Windows & Doors Ltd | Construction | 2025 |
| Dynasty Investment Group Ltd | Real estate | 2019 |
| Dynospeed Properties Ltd | Professional & scientific | 2023 |
| E G Compliance Services Limited | Professional & scientific | 2018 |
| E M P Solutions (Teesside) Ltd | Construction | 2015 |
| E&M Flounders Limited | Financial services | 2022 |
| E. V. a. Project Management Ltd | Construction | 2020 |
| E.C. Jarred (Guisborough) Limited | Wholesale & retail | 1983 |
| E.C.Jarred(M.S.S.)Limited | Wholesale & retail | 1972 |
| E.E.N. Solutions Limited | Health & social care | 2022 |
| E.G.A. (UK) Limited | Professional & scientific | 2006 |
| E.H.S. Solutions & Consulting Limited | Professional & scientific | 2016 |
| E.V. Industrial Services Limited | Mining & quarrying | 2019 |
| Eagle Chauffeuring Ltd | Transport & logistics | 2024 |
| Eagle Refractories Limited | Professional & scientific | 2009 |
| Eagle's Height Energy Solutions UK Limited | Professional & scientific | 2026 |
| Eaglescliffe Aesthetics Ltd | Health & social care | 2021 |
| Eaglescliffe GAS Limited | Construction | 2005 |
| Eaglescliffe Investments Limited | Professional & scientific | 2024 |
| Eaglescliffe Land Development Limited | Real estate | 2023 |
| Eaglescliffe Pet Care Ltd | Other services | 2026 |
| Eaglescliffe Properties Limited | Real estate | 2003 |
| Eaglescliffe Technical Solutions Ltd | Professional & scientific | 2018 |
| EAJ Property Limited | Real estate | 2024 |
| EAM Data Solutions Ltd | Information & communication | 2009 |
| Earlsdon-Associates Limited | Professional & scientific | 2009 |
| Earlsway Group Limited | Manufacturing | 2017 |
| Earlsway Solutions Limited | Manufacturing | 2012 |
| Earnest Management Limited | Other services | 2015 |
| Earthkote Ltd | Wholesale & retail | 2025 |
| EAS Mobiles Ltd | Administrative & support | 2020 |
| Easi Gears Limited | Manufacturing | 1995 |
| Easingwold Engineering Solutions Limited | Construction | 2016 |
| East Coast Keys Limited | Wholesale & retail | 2024 |
| East Durham Business Service Ltd. | Other services | 1986 |
| Eastend & Co Estates Limited | Real estate | 2020 |
| Eastern European Capital Limited | Professional & scientific | 2013 |
| Eastern European Trading Ltd | Professional & scientific | 2016 |
| Eastern Ravens Trust | Education | 1987 |
| Eastern Sunrise Bakery Ltd | Manufacturing | 2021 |
| Eastfield Finance Limited | Professional & scientific | 2008 |
| Eastview Health Care Ltd | Health & social care | 2023 |
| Eastview Stays Ltd | Hospitality | 2023 |
| Ebook Fusion Ltd | Information & communication | 2024 |
| Ebosoft Limited | Information & communication | 2010 |
| Eciggy UK Limited | Wholesale & retail | 2014 |
| Ecla Plumbing & Heating Ltd | Construction | 2020 |
| Eclectic Project EP Ltd | Wholesale & retail | 2020 |
| Eclipse Render Systems Limited | Construction | 2019 |
| Ecliptec Mobile Ltd | Information & communication | 2016 |
| Eco Connect North East CIC | Professional & scientific | 2024 |
| Eco Drill (England) Limited | Construction | 2007 |
| Eco Living Solutions Ltd | Wholesale & retail | 2019 |
| Ecobility Investments UK LLP | | 2025 |
| Ecoseal Windows and Doors NE Ltd | Construction | 2022 |
| Ecsgov Ltd | Financial services | 2024 |
| Ectobionics Ltd | Professional & scientific | 2026 |
| EDB Consultancy Limited | Professional & scientific | 2019 |
| Eddy Engineering Ltd | Professional & scientific | 2025 |
| Eden Hairdressing Ltd | Other services | 2026 |
| Eden Parc Ltd | Health & social care | 2012 |
| Eden Summer Land Developments Limited | Real estate | 2024 |
| Edge Squared Ltd | Real estate | 2014 |
| Edge Stockton Ltd | Hospitality | 2017 |
| Edgemoor Professional Ltd | Wholesale & retail | 2021 |
| Edmc Legal Limited | Professional & scientific | 2014 |
| Edmensons Limited | Professional & scientific | 2012 |
| Edmond & Alsop Ltd | Wholesale & retail | 2021 |
| Edps Properties Ltd | Real estate | 2024 |
| Education Video Solutions Ltd | Education | 2024 |
| Education Welfare Ltd | Education | 2011 |