| ABL Professional Services Ltd | Information & communication | 2010 |
| Action Adventure Activities Limited | Arts & recreation | 2005 |
| Action in Mind | Health & social care | 1994 |
| Airth Station Kennels Ltd | Other services | 2006 |
| Alex M Adamson LLP | | 2006 |
| Allan J. Gilmour Haulage Limited | Transport & logistics | 2002 |
| Annfield Cleaning Services Limited | Administrative & support | 2005 |
| Arthur Pollock and Company | | 1976 |
| Aura Design Interiors Ltd. | Other services | 2004 |
| Back O'Hill Farm | | 1986 |
| Balcorrach & Balgrochan Farms | | 1996 |
| Barclay Dougall (Holdings) Limited | Professional & scientific | 1998 |
| Bogton Farm | | 1986 |
| Braeview Properties Ltd | Real estate | 2026 |
| Burnhouse Game Farms Limited | Agriculture & fishing | 2002 |
| Burnhouse Lochan Fishery Limited | Agriculture & fishing | 1997 |
| CBS Electrical Services Limited | Construction | 2016 |
| Central Advocacy Partners | Health & social care | 2000 |
| Central Home Improvements Ltd. | Construction | 2002 |
| Chocktaw Resources Ltd | Information & communication | 2024 |
| Clan Distribution Limited | Professional & scientific | 1994 |
| Cosca (Counselling & Psychotherapy in Scotland) | Health & social care | 1993 |
| Dasherhead Farm | | 1979 |
| Digital Delivery Limited | Professional & scientific | 1996 |
| Drumhead Quarry Limited | Mining & quarrying | 2011 |
| Drumhead Sandstone Limited | Mining & quarrying | 2013 |
| Drumquhassle Farm | | 1985 |
| Duffer Limited | Information & communication | 2007 |
| E & O Diagnostics Limited | Professional & scientific | 2001 |
| E & O Laboratories Limited | Manufacturing | 1990 |
| Easystore Ltd. | Professional & scientific | 1972 |
| Ecurie Ecosse 2000 Limited | Arts & recreation | 2000 |
| Envigour | | 2006 |
| Express Shopping Limited | Professional & scientific | 1954 |
| Fair Deal Mortgages Ltd | Extraterritorial | 2007 |
| Fankerton Contracts Limited | Construction | 2014 |
| Fankerton Plant Limited | Construction | 2010 |
| Farmore I.T. Ltd. | Information & communication | 2003 |
| First Down Leisure Limited | Hospitality | 1988 |
| Gents @ 244 Limited | Other services | 2006 |
| George Russell Estates (Parkfoot) Ltd | Construction | 2024 |
| Glebe Fashion Ltd. | Wholesale & retail | 2003 |
| Glengyle Garage Limited | Wholesale & retail | 1976 |
| Good Entertainment Limited | Hospitality | 1992 |
| Greyrigg Systems Scotland Limited | Information & communication | 1998 |
| H & J Burgoyne (Van Sales) Limited | Wholesale & retail | 2007 |
| H & J Burgoyne Limited | Wholesale & retail | 2006 |
| Hardiston Farmers | | 1995 |
| Heat for Hire (Scotland) Limited | Extraterritorial | 1977 |
| Horse HOW to Academy Ltd | Agriculture & fishing | 2011 |
| Indigo Sun Retail Ltd. | Other services | 1990 |
| Infinity Telematics Ltd | Information & communication | 2019 |
| Innovene Trustee Limited | Financial services | 2006 |
| J & J Doyle Limited | Wholesale & retail | 2005 |
| J D Joinery Bonnybridge Limited | Manufacturing | 2004 |
| J.P. Rentals Limited | Administrative & support | 2001 |
| J.W. Mcewan (Farms) | | 1984 |
| James Bain & Co. Ltd. | Professional & scientific | 1999 |
| James Strang Limited | Construction | 1986 |
| JH Kilt Hire Limited | Wholesale & retail | 2002 |
| John Findlay Russell (Investments) Limited | Real estate | 1965 |
| JS Property (Scotland) Limited | Real estate | 2004 |
| Kerr Stirling LLP | | 2005 |
| Kings Park Financial Management (Scotland) Limited | Financial services | 2004 |
| Laird & Grant Ltd | Wholesale & retail | 2018 |
| Legge Steel (Fabrications) Limited | Manufacturing | 1984 |
| Lhanbryde Operations Ltd | Administrative & support | 2013 |
| Lion & Unicorn Hotel Limited | Hospitality | 1999 |
| LM Heights Ltd | Construction | 2024 |
| Lomond Offshore Services Ltd | Mining & quarrying | 2004 |
| Manor Hall Specialist Care Partnerships Limited | Public administration | 2013 |
| Marina Executive Travel Ltd. | Transport & logistics | 2001 |
| Marine Offshore Design Limited | Professional & scientific | 2007 |
| Mark of Millennium Ltd. | Wholesale & retail | 1999 |
| Masrose Media Ltd | Wholesale & retail | 2025 |
| MVB Commercial Services Ltd | Professional & scientific | 2003 |
| Nelsons Limited | Real estate | 1990 |
| Nord Digital Ltd | Professional & scientific | 2020 |
| Officepace Limited | Administrative & support | 2023 |
| Paterson Garden Buildings Limited | Manufacturing | 2007 |
| People Construction Limited | Administrative & support | 2005 |
| Petroineos Manufacturing Scotland Limited | Manufacturing | 1919 |
| Phil Brett Consulting Limited | Professional & scientific | 2007 |
| Plean Precast Limited | Manufacturing | 1967 |
| Polygon Damage Control (Scotland) Limited | Administrative & support | 2002 |
| Powdrake Servicentre Ltd | Wholesale & retail | 2004 |
| R. Lindsay and Company (Contractors) Limited | Construction | 1977 |
| Radio Music Store Limited | Wholesale & retail | 1974 |
| Recognise-Me Limited | Wholesale & retail | 2005 |
| Researchology Limited | Professional & scientific | 2003 |
| Retornados Ltd | Hospitality | 2013 |
| Robert Williamson (Developments) Limited | Construction | 1984 |
| Russell of Larbert Limited | Wholesale & retail | 1945 |
| S. Blackwood Consultants Limited | Information & communication | 1996 |
| Scott Macbride Coats Limited | Administrative & support | 2003 |
| Smith Packaging Products Limited | Manufacturing | 2005 |
| Sovereign Eyecare Ltd | Wholesale & retail | 2004 |
| Springboard Events Limited | Administrative & support | 2007 |
| Steve Easton Engineering Limited | Professional & scientific | 2001 |
| Stewart & Co | | 1986 |