| Unotus Ltd | Information & communication | 2019 |
| UOS Education Limited | Other services | 2011 |
| Uphall Estates Ltd | Real estate | 2007 |
| Upvc Repairs Ltd | Construction | 2025 |
| UQ Consulting Limited | Administrative & support | 2009 |
| Utilities Deals Ltd | Information & communication | 2025 |
| Uzotex African Global Limited | Wholesale & retail | 2026 |
| V B S Centurion Blinds Ltd | Manufacturing | 2008 |
| V R Management Consultancy Ltd. | Administrative & support | 2005 |
| VA Superfoods Ltd | Wholesale & retail | 2024 |
| Val's Garage Ltd | Wholesale & retail | 2021 |
| Vanguard Media Ltd | Wholesale & retail | 2019 |
| Vapes on TAP Ltd | Wholesale & retail | 2026 |
| Vartal Trading LP | | 2016 |
| Vault 101 Limited | Wholesale & retail | 2015 |
| VD Groundworks Ltd | Construction | 2022 |
| Venachar Cabins Ltd | Hospitality | 2019 |
| Venona Consult LP | | 2016 |
| Vera Artisan Bakery Limited | Manufacturing | 2019 |
| Veritech International LP | | 2017 |
| VG Tennis Limited | Arts & recreation | 2024 |
| VGM Holdings Ltd | Real estate | 2023 |
| Vi-Service.Sc Ltd | Construction | 2021 |
| Vialii Gardens Ltd | Professional & scientific | 2010 |
| Vibe (Glasgow) Ltd | Real estate | 2022 |
| Vibrant Resolution Limited | Administrative & support | 2019 |
| Vict Access Limited | Information & communication | 2015 |
| Victoria's Aesthetics Ltd | Health & social care | 2016 |
| View Referrals Ltd | Professional & scientific | 2025 |
| Vijayanand Ltd | Wholesale & retail | 2021 |
| Viking Medical Services Ltd | Health & social care | 2023 |
| Villette Impex LP | | 2016 |
| Virgil Ltd. | Real estate | 2010 |
| Viridis Building Services (Scotland) Ltd | Professional & scientific | 2016 |
| Visma Foods Ltd | Hospitality | 2018 |
| Vitalink Integrity Merchant Partners | | 2009 |
| Vitality Electrical Ltd | Construction | 2024 |
| Vitality Property and Refurbishments Ltd | Construction | 2021 |
| Vitality Property Group Limited | Real estate | 2024 |
| Vitality Property Ltd | Real estate | 2021 |
| Vito Holdings Limited | Professional & scientific | 2025 |
| Vivid Affect Productions Ltd | Information & communication | 2018 |
| Vizion 4 Ltd | Construction | 2023 |
| Vlad Marine LP | | 2013 |
| VOL Invest LP | | 2017 |
| Volante Properties Ltd | Real estate | 2014 |
| Volunteer Development Scotland Limited | Information & communication | 1987 |
| W & L Hunter Industrial Services Ltd | Administrative & support | 2014 |
| W S Dunsire & Sons Limited | Construction | 1991 |
| W S Dunsire Investments Ltd | Construction | 2025 |
| W. James Campbell Limited | Construction | 2005 |
| W. Somers and Currid Stirling Limited | Other services | 2008 |
| W. T. Paterson Joiners & Builders Ltd | Administrative & support | 2015 |
| Wackwell Lend LP | | 2014 |
| Wadaanwazir Ltd | Health & social care | 2025 |
| Wallace DS Limited | Professional & scientific | 2017 |
| Wallace Group Limited | Construction | 2003 |
| Wallace Property Solutions Ltd | Real estate | 2024 |
| Walmer Limited | Hospitality | 1995 |
| Walton Kilgour Stirling Ltd. | Professional & scientific | 2008 |
| Ward Castle Ltd | Professional & scientific | 2020 |
| Warm Eco Homes Limited | Construction | 2015 |
| Water Systems (Scotland) Ltd. | Wholesale & retail | 2003 |
| Watret Properties Ltd | Real estate | 2021 |
| Watson Stonecraft Limited | Construction | 2008 |
| Wayline Limited | Real estate | 2025 |
| Wbmh Electrical & Services Scotland Ltd | Construction | 2022 |
| WE ARE One Wedding Films Ltd | Professional & scientific | 2023 |
| WE ARE Stirling Ltd | Manufacturing | 2023 |
| Web ALT Limited | Wholesale & retail | 2023 |
| Web Pharmacy Limited | Wholesale & retail | 2009 |
| WEE Event Company Ltd | Arts & recreation | 2023 |
| Weiland LP | | 2016 |
| West END Garage Buchlyvie Limited | Wholesale & retail | 2018 |
| West Lodge Services Ltd | Professional & scientific | 2022 |
| West Summit Limited | Information & communication | 2026 |
| Westerlea Properties (Scotland) Limited | Construction | 2020 |
| Wetwall Wonders Ltd | Wholesale & retail | 2025 |
| Wext Media Technologies LP | | 2016 |
| White Dove Coffee Roasters Port Street Ltd | Hospitality | 2025 |
| Whitefield Investments Limited | Real estate | 2020 |
| Whitelaw Honeyman Holdings Ltd | Professional & scientific | 2015 |
| Whitton Technical Services Limited | Professional & scientific | 2003 |
| Wiayu Global Concept Limited | Wholesale & retail | 2026 |
| Wild Child Animation Witw Limited | Arts & recreation | 2021 |
| Wild Child Coba SPV Ltd | Arts & recreation | 2025 |
| Wild Heat (Stirling) Limited | Arts & recreation | 2026 |
| Wild Wellies Kindergarten Ltd | Education | 2020 |
| Wildial Limited | Information & communication | 2026 |
| Wildlens Retreats Ltd | Professional & scientific | 2025 |
| Wildside Adventures Ltd | Education | 2015 |
| William M. Beaton and Sons Limited | Construction | 2003 |
| William Paterson Livestock Ltd | Agriculture & fishing | 2019 |
| William Simpsons | Health & social care | 2010 |
| William Wallace Hostel Ltd | Hospitality | 2021 |
| Wills Window Cleaning Ltd | Administrative & support | 2025 |
| Windhelm OPS Limited | Transport & logistics | 2026 |
| Winford Trading LP | | 2014 |
| Winmore Alliance LP | | 2016 |
| Winslow West Private LP | | 2016 |