| Stirling Stove Installations Ltd | Construction | 2025 |
| Stirling Tailors Ltd. | Administrative & support | 1999 |
| Stirling Technology Projects Limited | Real estate | 1998 |
| Stirling Traders Ltd | Wholesale & retail | 2026 |
| Stirling Trailer Centre Limited | Wholesale & retail | 2012 |
| Stirling Turkish Barbers Ltd | Administrative & support | 2019 |
| Stirling University Innovation Park Limited | Real estate | 1985 |
| Stirling University Residential Accommodation Limited | Administrative & support | 2002 |
| Stirling Welcome Ltd | Hospitality | 2025 |
| Stirling Work Productions Limited | Information & communication | 2007 |
| Stirlingshire Saddle Fitters LLP | | 2010 |
| Stirlingshire Voluntary Enterprise Ltd. | Administrative & support | 2010 |
| Stitchforge Supply Ltd | Wholesale & retail | 2025 |
| Stoic Works Ltd | Administrative & support | 2022 |
| Stone Cold Steve Autos Ltd | Wholesale & retail | 2026 |
| Stone Design Limited | Construction | 1990 |
| Stonerigg Construction Scotland Ltd | Construction | 2023 |
| Storage Division Ltd | Transport & logistics | 2024 |
| Stout Livestock Consulting Ltd | Professional & scientific | 2025 |
| Straker Systems Limited | Information & communication | 2012 |
| Stranput Consulting | | 2009 |
| Strathallan Meeting, Limited | Arts & recreation | 1908 |
| Strathard Community Trust Limited | Other services | 2005 |
| Stratli Ltd | Financial services | 2026 |
| Stravaig Ltd | Wholesale & retail | 2022 |
| Strevelin Homes Limited | Real estate | 2022 |
| Stronger Health | | 2009 |
| Struthersmedia Ltd | Arts & recreation | 2026 |
| STU Thomson Ltd | Professional & scientific | 2026 |
| Stuart Burge Partnership Ltd | Arts & recreation | 2011 |
| Stuart Lennon Service Limited | Professional & scientific | 2023 |
| Stuart Maclaren Plumbing & Heating Ltd. | Construction | 2005 |
| Stuart Titles Limited | Information & communication | 1983 |
| Studio 52 Limited | Professional & scientific | 2012 |
| Studio Tristram Limited | Arts & recreation | 2010 |
| Stuf! Marketing Limited | Wholesale & retail | 2013 |
| Style Decorators Ltd. | Construction | 2002 |
| Style Scotland Limited | Construction | 2007 |
| Subbureddiar Property Group Ltd | Real estate | 2025 |
| Subliminal Events Ltd | Administrative & support | 2020 |
| Sughran Ltd | Transport & logistics | 2021 |
| Suitecrm Ltd | Information & communication | 2024 |
| SUJ IT Solutions Limited | Administrative & support | 2010 |
| Sumond Trading LP | | 2016 |
| Sun Systems Limited | Wholesale & retail | 1989 |
| Sun WAH Mini-Mart Ltd | Wholesale & retail | 2014 |
| Sunnylaw Consulting Limited | Administrative & support | 2023 |
| Sunnyport Holding LP | | 2016 |
| Sunoh Edinburgh Ltd | Information & communication | 2018 |
| Sunrite Blinds Limited | Wholesale & retail | 2015 |
| Supasites Ltd | Information & communication | 2025 |
| Superico Limited | Hospitality | 2015 |
| Superior Sports Management Limited | Professional & scientific | 2013 |
| Sure Fit Cars and Commercial Ltd | Wholesale & retail | 2021 |
| Surefix Signs (Scotland) Ltd. | Construction | 2002 |
| Suspend Fitness Stirling Ltd | Arts & recreation | 2025 |
| Sustainable Management (UK) Limited | Administrative & support | 2015 |
| Sutherland Blinds Ltd. | Extraterritorial | 2003 |
| Sutherland Renewables Ltd | Professional & scientific | 2023 |
| Svedoline Management LP | | 2016 |
| SVP Glasgow Ltd | Hospitality | 2024 |
| SW Facilities (Scotland) Ltd | Real estate | 2022 |
| SW Interactive Ltd | Information & communication | 2026 |
| Swano Logistics LP | | 2016 |
| Sweet Little Cheesecake Creations Ltd | Hospitality | 2022 |
| Swilken Developments Limited | Construction | 2008 |
| Swilken Estates Limited | Financial services | 2012 |
| Swilken Homes Limited | Real estate | 2021 |
| Swimsara Swimwear Ltd | Wholesale & retail | 2024 |
| Sydna International LP | | 2016 |
| Symbiosis Holdings (Scotland) Ltd | Professional & scientific | 2020 |
| Symbiosis Pharmaceutical Services Limited | Manufacturing | 2010 |
| Synergy Solutions Consultancy Ltd | Professional & scientific | 2024 |
| T & J Gilliland | | 1999 |
| T Properties Ltd. | Hospitality | 2008 |
| T&Tqs Ltd | Construction | 2010 |
| T. Laurie Electrical Limited | Construction | 2025 |
| Tabago Impex LP | | 2016 |
| Tades Europe LP | | 2016 |
| Tagran Trading LP | | 2018 |
| TAI CHI Consulting Limited | Other services | 2025 |
| TAI CHI Spa Centre UK Ltd | Health & social care | 2012 |
| Taic Solutions Limited | Professional & scientific | 2019 |
| Tailored Electrical Services Limited | Construction | 2017 |
| Tailormade Nails Limited | Wholesale & retail | 2008 |
| Taleka Group Limited | Administrative & support | 2010 |
| Taleka Limited | Administrative & support | 2006 |
| Talent Strengths Ltd | Administrative & support | 2011 |
| Talking Mats Ltd. | Health & social care | 2011 |
| Tangerine Industries Limited | Real estate | 1989 |
| Tannery Treasures Ltd | Wholesale & retail | 2025 |
| Target Advisers LLP | | 2010 |
| Target Fund Managers Limited | Financial services | 2016 |
| Tartan Box Ltd | Professional & scientific | 2019 |
| Tartan Services Ltd | Public administration | 2015 |
| Tarways Ltd | Construction | 2023 |
| Taste Base Limited | Hospitality | 2019 |
| Tatterdemalion Blue Limited | Information & communication | 2012 |
| Tattie & Toasted Limited | Hospitality | 2025 |
| TAU Telecom LP | | 2010 |