| Ecom Prime UK Ltd | Wholesale & retail | 2026 |
| Economic Development Association (Scotland) | Other services | 1999 |
| Economics Research Ltd | Professional & scientific | 2020 |
| Ecosse Leisure Limited | Hospitality | 1999 |
| Ecot Renewables Group Ltd | Professional & scientific | 2024 |
| Ecot Renewables Ltd | Professional & scientific | 2021 |
| ED Design Ltd | Professional & scientific | 2021 |
| Ed's FUT Cards Ltd | Wholesale & retail | 2025 |
| EDA Homes Ltd | Real estate | 2024 |
| Eden-Rose Events Ltd | Administrative & support | 2023 |
| EDGE2EDGE Roofing Ltd | Construction | 2025 |
| Edinburgh Decor Ltd | Construction | 2016 |
| Edinburgh Highland Games Ltd | Arts & recreation | 2026 |
| Edinburgh Self Store Limited | Transport & logistics | 2021 |
| Edinmac Limited | Financial services | 2025 |
| Editor Holding LP | | 2017 |
| Eekhan Ltd | Wholesale & retail | 2023 |
| Effective Design Consultants Limited | Professional & scientific | 2008 |
| Eilidh Brown Memorial Fund | Hospitality | 2011 |
| EKR Solutions Ltd | Construction | 2024 |
| EL Two Limited | Real estate | 2015 |
| El-Roi Tradehub UK Ltd | Wholesale & retail | 2025 |
| Elderbrae Limited | Real estate | 2004 |
| Electra Capital LP | | 2014 |
| Element Consultancy and Training Ltd | Education | 2026 |
| Elemental Systems Ltd | Construction | 2025 |
| Elite Construction & Forecourt Services Ltd | Construction | 2022 |
| Elite Joinery Construction Ltd | Construction | 2023 |
| Eliteshopfusion Ltd | Wholesale & retail | 2024 |
| Elixor Solutions Ltd | Real estate | 2018 |
| Elmview Investments Ltd | Real estate | 2025 |
| ELR GAS Ltd | Construction | 2021 |
| Elston Roofing Services Ltd | Construction | 2026 |
| ELU Images Limited | Administrative & support | 2013 |
| Elvara Capital Limited | Real estate | 2025 |
| Elysium Joinery Ltd | Manufacturing | 2024 |
| Emblation Limited | Manufacturing | 2008 |
| Emcam Ltd | Hospitality | 2021 |
| Emet Trading LP | | 2016 |
| Emihol Investments Limited | Real estate | 2020 |
| Empawrium Services Limited | Wholesale & retail | 2024 |
| Empire Cake Studio Ltd | Wholesale & retail | 2026 |
| Empire Exports Ltd | Wholesale & retail | 2025 |
| Empire Inns Property Company Limited | Hospitality | 2018 |
| Empire Inns Stirling Limited | Hospitality | 2018 |
| Empirica Research & Consulting Ltd | Professional & scientific | 2025 |
| Empower Contracting Ltd | Professional & scientific | 2015 |
| Enabling Company Ltd | Administrative & support | 2007 |
| Endeavour Hospitality Limited | Hospitality | 2019 |
| Energy Diagnostics Limited | Professional & scientific | 2010 |
| Enterprise C-D Limited | Other services | 2014 |
| Environmental Dust Solutions Limited | Manufacturing | 2008 |
| Environmental Facility Management Ltd | Administrative & support | 2023 |
| Envision (Contemporary Interiors) Ltd. | Construction | 1998 |
| EP Learning Stirling Ltd | Education | 2024 |
| Epic 7 Limited | Extraterritorial | 2020 |
| Epic Playbooks Ltd | Administrative & support | 2011 |
| Epic Scotland Limited | Professional & scientific | 2001 |
| Epion Technologies Ltd | Manufacturing | 2025 |
| Erik J Mcmartin Ltd | Manufacturing | 2025 |
| Eros Events Ltd | Hospitality | 2021 |
| Escape, Health and Wellness Ltd | Health & social care | 2024 |
| ESL Solutions Ltd | Real estate | 2026 |
| ESP (Trustees) Limited | Professional & scientific | 2011 |
| ESP Administration Services Limited | Administrative & support | 2022 |
| Estate and Succession Planning (Trustees) Ltd | Professional & scientific | 2005 |
| Ethleon Ltd | Wholesale & retail | 2025 |
| Etre Beau Ltd | Other services | 2010 |
| Ettrick Trout Co. Limited | Agriculture & fishing | 1978 |
| Eurasia Transit LP | | 2012 |
| Eurodome Ltd | Construction | 2011 |
| Eurotech Alliance LP | | 2014 |
| Eurotech Engineers Limited | Manufacturing | 2025 |
| Eurotrans Capital LP | | 2016 |
| EVA Green Properties Ltd | Real estate | 2023 |
| Evan Fitness Ltd | Arts & recreation | 2018 |
| Events at Luna Limited | Hospitality | 2022 |
| Everett Properties Limited | Construction | 1990 |
| Eversmart Wealth LP | | 2016 |
| Evertech Industries LP | | 2016 |
| Evoltech Limited | Professional & scientific | 2013 |
| EVRU2022 Ltd | Professional & scientific | 2022 |
| Exact Method Company LP | | 2016 |
| Excellent Tailors Limited | Manufacturing | 2014 |
| Executive Golf & Leisure Limited | Administrative & support | 1996 |
| Exelex Business LP | | 2016 |
| Expert IT Solutions Ltd | Information & communication | 2002 |
| Expotrade Global LP | | 2016 |
| Extra Trials Estate LP | | 2016 |
| Extremis Publishing Limited | Information & communication | 2015 |
| EZ Cloudtech Ltd | Information & communication | 2016 |
| F Brown Scotland Limited | Construction | 2019 |
| Fablehub Ltd | Wholesale & retail | 2026 |
| Fabyl Sales LP | | 2017 |
| Faello Trade LP | | 2016 |
| Fair Buildings Ltd | Construction | 2016 |
| Fairdeal Autos (Plean) Ltd | Wholesale & retail | 2019 |
| Fairdeal Autos (Stirling) Ltd | Wholesale & retail | 2014 |
| Fairy Rings Ltd | Agriculture & fishing | 2026 |
| Falconer Property Group Ltd | Real estate | 2025 |