| 13 RAW Property Ltd | Real estate | 2022 |
| 1314 Group Limited | Construction | 2025 |
| 1688 Trading Limited | Hospitality | 2019 |
| 1ST Response Group Ltd | Water & waste | 2019 |
| 21 LUX Motorsport Ltd | Wholesale & retail | 2024 |
| 222 Advisory Ltd | Professional & scientific | 2025 |
| 24GB Limited | Information & communication | 2025 |
| 2BROTHERSCHILOS Limited | Hospitality | 2024 |
| 2E-VOLVE (UK) Ltd | Information & communication | 2004 |
| 3060 Marketing Ltd | Professional & scientific | 2024 |
| 333 Services Limited | Wholesale & retail | 2007 |
| 333 Spice Ltd | Hospitality | 2025 |
| 360 Business Viewpoint Ltd | Financial services | 2018 |
| 360 Viewpoint Ltd | Financial services | 2018 |
| 3D Electrical & Security Limited | Construction | 2018 |
| 4 UR Paws Only Ltd. | Agriculture & fishing | 2007 |
| 4NIL Research and Production Ltd | Information & communication | 2016 |
| 4WM Ltd | Wholesale & retail | 2025 |
| 588 Trading Limited | Hospitality | 2025 |
| 5STARX Mystery Customer Experience Limited | Administrative & support | 2025 |
| 5TH Flavour Foods Ltd | Wholesale & retail | 2020 |
| 7 Days Roofing & Property Maintenance Ltd | Construction | 2018 |
| 786 Enterprises Ltd | Wholesale & retail | 2019 |
| 83 Hanover Street Limited | Real estate | 2017 |
| 99 Hanover Street Limited | Real estate | 2018 |
| A & a Decorators Ltd | Construction | 2025 |
| A & C Cowan Properties Limited | Real estate | 2024 |
| A & D Blacksmiths Ltd. | Construction | 2013 |
| A & L Dixon Properties Limited | Real estate | 2022 |
| A Ahmed Construction Ltd | Construction | 2025 |
| A C Gold Forever Warm Limited | Professional & scientific | 2008 |
| A DNO Raploch Chippy Ltd | Hospitality | 2025 |
| A Fleetwood Property Limited | Real estate | 2024 |
| A Hunter Builders (Scotland) Limited | Construction | 2010 |
| A R Beattie and Company | | 1988 |
| A R Stores Ltd | Wholesale & retail | 2017 |
| A Sneddon Courier & Logistics Ltd | Administrative & support | 2026 |
| A&A Stores Ltd | Wholesale & retail | 2023 |
| A&H Plus L Ltd | Administrative & support | 2026 |
| A. Mccallum (Properties) Limited | Real estate | 1993 |
| A.M&H Consultancy Limited | Wholesale & retail | 2023 |
| A.N.I Solutions Ltd | Construction | 2024 |
| A.T.H Instruments Ltd | Professional & scientific | 2006 |
| A1BION Scotland LP | | 2023 |
| AAC Stirling Ltd | Wholesale & retail | 2019 |
| AAP Distribution Ltd | Wholesale & retail | 2024 |
| AAS Group Limited | Professional & scientific | 2017 |
| AAS Mccabe Limited | Transport & logistics | 2017 |
| ABA Style Ltd | Other services | 2026 |
| Abbey Antiques (Scotland) Ltd | Wholesale & retail | 2024 |
| Abbey Digital Ltd | Information & communication | 2019 |
| Aberdeen Blind Company Limited | Manufacturing | 1991 |
| Aberfoyle Delicatessen Limited | Wholesale & retail | 2019 |
| Aberfoyle Leisure Ltd | Hospitality | 2025 |
| Aberlour Child Care Trust | Other services | 2006 |
| ABH Surfacing Ltd | Construction | 2022 |
| Abraservice Scotland Ltd | Manufacturing | 1997 |
| AC Electrical Scotland Limited | Construction | 2025 |
| AC Gold Electrical Services Limited | Construction | 2008 |
| AC Gold Energy Limited | Extraterritorial | 2014 |
| AC Gold Holdings Limited | Financial services | 2018 |
| AC Service Solutions Limited | Wholesale & retail | 2026 |
| Access AV Limited | Information & communication | 2008 |
| ACF Cleaning Limited | Other services | 2002 |
| ACF Properties Limited | Hospitality | 2008 |
| Ackc Cricket Ltd | Arts & recreation | 2018 |
| ACM Wealth Limited | Financial services | 2025 |
| Acoustic Design Ltd | Other services | 2009 |
| Acrux Group Ltd | Wholesale & retail | 2023 |
| ACS Biomass Ltd | Energy | 2011 |
| Active Air Conditioning Limited | Energy | 1999 |
| Active Network Management Limited | Administrative & support | 2016 |
| Active Stirling Limited | Arts & recreation | 2006 |
| Active Stirling Trading Limited | Other services | 2007 |
| Active-X Management LP | | 2016 |
| Acts Scotland CIC | Education | 2017 |
| AD Blade Repairs Ltd | Information & communication | 2019 |
| Adam Arkell EMC Ltd | Professional & scientific | 2025 |
| ADC Business Services Limited | Construction | 2025 |
| ADD Contracts (Civils) Ltd | Professional & scientific | 2023 |
| Adegrity Ltd | Professional & scientific | 2022 |
| Administrative Group LP | | 2016 |
| ADO Construction Limited | Financial services | 2004 |
| Adrian Condrea Ltd | Transport & logistics | 2018 |
| Advance Cabling Solutions Ltd | Information & communication | 2017 |
| Advanced Craft Joiners Ltd | Construction | 2023 |
| Advanced Material Processing Limited | Mining & quarrying | 2015 |
| Advanced Perimeter Systems Limited | Manufacturing | 1996 |
| Advanced Whitening Ltd | Wholesale & retail | 2018 |
| Adventure Day Projects LP | | 2016 |
| Adventure Works Limited | Hospitality | 2025 |
| Aege Continuity (UK) Ltd | Wholesale & retail | 2025 |
| Aege Energy (UK) Ltd | Construction | 2021 |
| Aeme Scotland Limited | Professional & scientific | 2014 |
| Aequitas Black Limited | Professional & scientific | 2025 |
| Aequitas Investments Group Limited | Real estate | 2026 |
| Aesthetique by Dominique Ltd | Other services | 2022 |
| Aestimare Ltd | Other services | 2025 |
| AFG Stirling Limited | Real estate | 2000 |
| AFS (Scotland) Limited | Construction | 2001 |