| Essex Storage Limited | Transport & logistics | 2023 |
| EST Media Limited | Administrative & support | 2021 |
| Estate Agency X Limited | Administrative & support | 2026 |
| Estate Cleaning and Landscapes Limited | Administrative & support | 2012 |
| Esther Ayensu Medical Services Ltd | Health & social care | 2021 |
| ETF Trading & Services Ltd | Professional & scientific | 2020 |
| Eton Property Lettings Limited | Hospitality | 2020 |
| ETS Lindgren Limited | Manufacturing | 1967 |
| Etsm GAS Ltd | Construction | 2025 |
| Ettesmeg Limited | Health & social care | 2025 |
| Etyprises Properties Limited | Real estate | 2021 |
| Euco Ltd | Financial services | 2018 |
| Eurasian Eagle OWL Ltd | Information & communication | 2022 |
| Euro Diagnostic & Surgical Equipments (Edse) Ltd | Manufacturing | 2020 |
| Euro Exotics Ltd | Wholesale & retail | 2021 |
| Euro Holography Ltd | Professional & scientific | 2017 |
| Euro VID Consulting Limited | Administrative & support | 2023 |
| Eurochange Limited | Administrative & support | 1990 |
| Euroden 2 Ltd | Health & social care | 2019 |
| Euroden Holdings Ltd | Financial services | 2019 |
| Euroden Limited | Health & social care | 2010 |
| Europe Voyages Ltd | Administrative & support | 2019 |
| Eurozam Resourcing Ltd | Wholesale & retail | 2012 |
| Evans Wood Finishing Limited | Administrative & support | 2003 |
| Evelrani Services Ltd | Health & social care | 2024 |
| Event Profs Staffing Ltd | Administrative & support | 2024 |
| Eventify Ltd | Administrative & support | 2017 |
| Eventis Group Ltd | Arts & recreation | 2026 |
| Events Box Hospitality Limited | Hospitality | 2024 |
| Everchanging Design Commercial Ltd | Administrative & support | 2021 |
| Everchanging Design Limited | Administrative & support | 2018 |
| Everclean Holdings Ltd | Administrative & support | 2025 |
| Evergood Health and Social Care Limited | Administrative & support | 2021 |
| Evergreen Facilities Services Limited | Administrative & support | 1992 |
| EVO Environmental Ltd | Professional & scientific | 2017 |
| Evolene Skye Limited | Education | 2012 |
| Excalibur UK Group Holdings Limited | Professional & scientific | 2019 |
| Exceedo Ltd | Financial services | 2017 |
| Excel Advance Trim Ltd | Manufacturing | 2021 |
| Excelpro Consulting Financial Services UK Limited | Professional & scientific | 2024 |
| Exciter Freelancing Ltd | Professional & scientific | 2024 |
| Exclusive Insulation Ltd | Information & communication | 2023 |
| Exclusive Interiors Limited | Professional & scientific | 2017 |
| Executive Cars Stevenage Limited | Transport & logistics | 2015 |
| Executive Office Solutions Limited | Administrative & support | 2012 |
| Executive Roofing & Sons Limited | Construction | 2026 |
| Exile Dubz Ltd | Manufacturing | 2022 |
| Experience Southwest Limited | Other services | 2018 |
| Expert Moving Services Limited | Transport & logistics | 2015 |
| Expo Portable Limited | Administrative & support | 2016 |
| Expose London Ltd | Real estate | 2016 |
| Expositionists International LLP | | 2009 |
| Expositionists Ltd | Administrative & support | 2025 |
| Express Dream Limited | Hospitality | 2024 |
| Express Search Ltd | Construction | 2019 |
| Extra Time Management Limited | Professional & scientific | 2016 |
| Extreme Pest Control Ltd | Manufacturing | 2018 |
| EZ Rental Limited | Real estate | 2024 |
| F a Consultants Limited | Health & social care | 2022 |
| F Koldashi Ltd | Construction | 2025 |
| F Thorsteinsson & Co Ltd | Arts & recreation | 2018 |
| F&G Bajrami Construction Ltd | Construction | 2018 |
| F&S Entertainment Limited | Arts & recreation | 2023 |
| F. & L. Group Limited | Construction | 1988 |
| F. J. Rowley and SON Limited | Agriculture & fishing | 1978 |
| F.I. Holdings and Consultancy Limited | Information & communication | 2025 |
| Faber Flooring Limited | Construction | 2023 |
| Fabfolly Limited | Manufacturing | 2025 |
| Fabio's Gelato Holdings Limited | Financial services | 2022 |
| Facade Consult Ltd | Professional & scientific | 2019 |
| Face & Skin Limited | Health & social care | 2016 |
| Face Communications (2007) Limited | Administrative & support | 2007 |
| Face Technologies Ltd | Wholesale & retail | 2022 |
| Facilities Global Ltd | Administrative & support | 2025 |
| Fada Group Limited | Information & communication | 2025 |
| Fairlands Grill Ltd | Hospitality | 2025 |
| Fairlands Pre-School (Stevenage) CIC | Education | 2017 |
| Fairton Group Ltd | Other services | 2017 |
| Fairview Care & Housing Solutions Ltd | Professional & scientific | 2021 |
| Fairview Gardens (Stevenage) Management Limited | Households | 2018 |
| Falcon Drone Services Ltd | Professional & scientific | 2025 |
| Falconbird Limited | Health & social care | 2025 |
| Famzyela Ltd | Health & social care | 2024 |
| Farmer Giles Farmstead Limited | Agriculture & fishing | 1999 |
| Farrier Heights Management Company Limited | Real estate | 2018 |
| Faruk ALI Ltd | Hospitality | 2017 |
| Fast Connect Limited | Transport & logistics | 2025 |
| Fast Plumbers Ltd | Construction | 2013 |
| Fast Steel Erecting Limited | Construction | 2021 |
| Fastpay Solutions Ltd | Professional & scientific | 2023 |
| FAT DAD Productions Limited | Manufacturing | 2026 |
| Fcah Ltd | Wholesale & retail | 2011 |
| FCP Services Ltd | Construction | 2024 |
| FDM Protection Ltd | Financial services | 2024 |
| Fearless Community Limited | Information & communication | 2021 |
| Fearless Group Limited | Information & communication | 2019 |
| Fearless Talent Limited | Financial services | 2021 |
| Feel Hot Yoga Ltd | Arts & recreation | 2018 |
| Feel Wellbeing Cafe Limited | Arts & recreation | 2019 |
| Fego Care Solutions Ltd | Education | 2025 |