| Dovestone Properties Limited | Real estate | 2006 |
| Dower House (Stoke Park) Management Company Limited | Households | 2006 |
| Downton Estates Management Limited | Real estate | 2026 |
| Dracula’S Bite Street Food Ltd | Hospitality | 2025 |
| Dragonfly Decorating Ltd | Construction | 2025 |
| Drakefield Estates Limited | Real estate | 2023 |
| Dravixprotection Ltd | Administrative & support | 2025 |
| Drew O'Neill Ltd | Information & communication | 2018 |
| Driving School MK Limited | Education | 2024 |
| Drumhum Ltd | Arts & recreation | 2025 |
| Drxydistributors Ltd | Wholesale & retail | 2025 |
| Dury Service Limited | Extraterritorial | 2020 |
| Dwyer Engineering Ltd | Professional & scientific | 2025 |
| Dynamic Scaffolding Limited | Construction | 2009 |
| Dynasty Design Ltd | Information & communication | 2025 |
| E&J Electrical Limited | Construction | 2023 |
| E-Heat Plumbing & Heating Ltd | Construction | 2017 |
| Earthmind CIC | Information & communication | 2024 |
| East Fair Limited | Other services | 2025 |
| East Sussex Plastering & Building Ltd | Construction | 2024 |
| East Sussex Roofing Ltd | Construction | 2022 |
| East Sussex Wills Ltd | Professional & scientific | 2022 |
| Eastbourne Islamic Cultural Centre | Health & social care | 2013 |
| EBL DRY Lining Contractors Limited | Construction | 2017 |
| Echoestours.Com Limited | Administrative & support | 2026 |
| Eclectika UK Ltd | Information & communication | 2020 |
| Ed's Chauffeur Travel Ltd | Transport & logistics | 2026 |
| Ed's Plastering Ltd | Construction | 2023 |
| Eddystone Broadcast Limited | Manufacturing | 2010 |
| Eddystone Broadcast Manufacturing Limited | Manufacturing | 2002 |
| Eddystone Manufacturing Limited | Manufacturing | 2009 |
| Eden Flora Ltd | Wholesale & retail | 2025 |
| Edmando Merchandise Ltd | Wholesale & retail | 2024 |
| Edstolet Limited | Real estate | 2025 |
| EFB Assets Limited | Real estate | 2025 |
| Eighty Seven Productions Ltd | Information & communication | 2023 |
| EKM Veterinary Group Ltd | Professional & scientific | 2023 |
| Electronic Security Plus Limited | Administrative & support | 2003 |
| Elementiq Systems Ltd | Manufacturing | 2026 |
| Elita Labs Ltd | Manufacturing | 2022 |
| Elite Electrical Engineering Limited | Construction | 2015 |
| Ellison Close Management (South East) Limited | Extraterritorial | 2024 |
| ELM & Oak Developments Ltd | Real estate | 2026 |
| Elphick Utilities Ltd | Construction | 2025 |
| Elsinore Garage and Company Limited | Administrative & support | 1956 |
| Embassy Court (SE25) RTM Company Limited | Households | 2022 |
| Emmaus Hastings and Rother | Wholesale & retail | 2007 |
| Emporium Music Library Ltd | Information & communication | 2022 |
| Emsley Revill Ltd | Manufacturing | 2025 |
| Emted Carpentry Ltd | Manufacturing | 2025 |
| Emupud Limited | Real estate | 2006 |
| Encyclie Construction UK Ltd | Construction | 2024 |
| Energise Electricals Limited | Construction | 2017 |
| Engineer for Safety Limited | Professional & scientific | 2013 |
| Envision Composites Ltd | Manufacturing | 2023 |
| EOD Distribution Ltd | Manufacturing | 2019 |
| Epiworks Ltd | Professional & scientific | 2015 |
| Essentials Luxe Ltd | Wholesale & retail | 2024 |
| Essex County Construction Ltd | Construction | 2019 |
| Ethereal Apparel Ltd | Wholesale & retail | 2025 |
| Ethical Hour Ltd | Professional & scientific | 2018 |
| Eton Place Management Limited | Households | 2019 |
| EURO4 Enterprises UK Ltd | Wholesale & retail | 2019 |
| EVA Cakes Ltd | Manufacturing | 2019 |
| Evans Designs Ltd | Manufacturing | 2025 |
| Events Travel Limited | Transport & logistics | 2015 |
| Eversfield Freehold Management Ltd | Households | 2016 |
| Eversfield Maintenance Limited | Households | 2023 |
| Eversfield Property (Holdings) Limited | Real estate | 2002 |
| Eversfield Regulatory Limited | Information & communication | 2022 |
| Eversfield Supermarket Ltd | Wholesale & retail | 2024 |
| Everyone Sang Limited | Information & communication | 2022 |
| Ex-Mil Recruitment Limited | Administrative & support | 2005 |
| Example Facilities Limited | Administrative & support | 2016 |
| Excel Decorating Contractors Ltd. | Construction | 2011 |
| Excell Cable Jointing Services Ltd | Construction | 2021 |
| Exclusive Floor Restoration Ltd | Other services | 2006 |
| Exklusive Fussbodenveredelung Ltd. | Construction | 2007 |
| EXP Video Ltd | Information & communication | 2025 |
| Explorethearch Theatre Company Ltd | Arts & recreation | 2017 |
| F & Lou’S Ltd | Hospitality | 2025 |
| F H Diversified Limited | Professional & scientific | 2018 |
| F K Consultancy Limited | Manufacturing | 2012 |
| Fab-Inns Limited | Hospitality | 2003 |
| Fairbook Accounting Solutions Ltd | Professional & scientific | 2008 |
| Fairfield IT Solutions Limited | Information & communication | 2015 |
| Fairmount Freehold Limited | Households | 2017 |
| Fairmount Park Limited | Hospitality | 2015 |
| Faisalnawaz Ltd | Wholesale & retail | 2025 |
| Falbrook Limited | Administrative & support | 2007 |
| Farthing Media Limited | Information & communication | 2010 |
| Farzana Food Market Ltd | Wholesale & retail | 2025 |
| Fashion Fabrics Limited | Wholesale & retail | 1991 |
| Fastprint & Design Limited | Manufacturing | 2006 |
| Felidae Limited | Transport & logistics | 2025 |
| Fermain House Limited | Households | 1994 |
| Ferns a Flamingo Ltd | Hospitality | 2017 |
| Fernside House Management Limited | Households | 2006 |
| FGM Engineering Limited | Information & communication | 2000 |
| FGS Group Limited | Professional & scientific | 2003 |