| Mindy Capital LP | | 2017 |
| Miramex LP | | 2015 |
| Mirton Corp. LP | | 2015 |
| Mission Hall Limited | Real estate | 1988 |
| Mitchell & Martin Ltd | Hospitality | 2016 |
| MMS Dynasty Properties Ltd | Real estate | 2025 |
| Monarch Holdings (Scotland) Ltd | Transport & logistics | 2024 |
| Monrose Property Ltd | Real estate | 2026 |
| Montpelier ROW 94 Ltd | Construction | 2018 |
| Motatest Limited | Professional & scientific | 2026 |
| Motorhome Adventures Scotland Ltd | Administrative & support | 2026 |
| MT Nails & Spa Ltd | Other services | 2024 |
| Muir Walker & Pride Ltd. | Administrative & support | 2006 |
| Mulligans of ST Andrews Ltd | Manufacturing | 2025 |
| Naturity Health Shop Limited | Wholesale & retail | 2021 |
| Navona Enterprises Limited | Other services | 2015 |
| Nerova LP | | 2015 |
| Netherlea Nursery Ltd | Education | 2016 |
| Neuk Holdings Ltd | Hospitality | 2025 |
| New Delight Takeaway (Fife) Ltd | Hospitality | 2023 |
| New Frontier Labs Ltd | Information & communication | 2025 |
| Newburgh Community Trust | Other services | 2005 |
| Niven & Co Ltd | Real estate | 2023 |
| North East Fife Community Hub | Hospitality | 2018 |
| Novaspectral AI Limited | Manufacturing | 2026 |
| Obzolete.Com Limited | Wholesale & retail | 2012 |
| Oldpals (20250612) Limited | Professional & scientific | 2017 |
| Oleva Group Ltd | Hospitality | 2024 |
| Omideon Limited | Professional & scientific | 2020 |
| ON Cctv Ltd. | Hospitality | 2006 |
| ONA Group Limited | Information & communication | 2024 |
| Onomix Media Ltd | Wholesale & retail | 2023 |
| Openside Sports Limited | Professional & scientific | 2005 |
| Oranmore Developments Ltd | Construction | 2018 |
| Ordinal Tennis Ltd | Wholesale & retail | 2024 |
| Osgoode Restoration of ST. Andrews Ltd | Construction | 2019 |
| Ostium Technology Ltd | Administrative & support | 2022 |
| Ouyang & Sun Property Group Ltd | Real estate | 2025 |
| Ouyang Global Trading Ltd | Wholesale & retail | 2026 |
| P & M Monteath Investments Ltd | Administrative & support | 2015 |
| Paedzwaze Ltd | Education | 2018 |
| Palea Systems Limited | Information & communication | 2018 |
| Paradegolf Ltd. | Administrative & support | 2018 |
| Parkdrum Limited | Professional & scientific | 2013 |
| Patersons Papers Plus Ltd | Wholesale & retail | 2018 |
| Paul Muir Technical Auditor Ltd | Professional & scientific | 2019 |
| Peaballs Ltd | Real estate | 2012 |
| Peter Dymoke Subcontracting Ltd | Professional & scientific | 2024 |
| PG Conseil UK Ltd | Professional & scientific | 2024 |
| Philanthropy Scotland Limited | Professional & scientific | 2011 |
| Photosynergy Limited | Professional & scientific | 2001 |
| Pilgrim Care | Health & social care | 1995 |
| Pink Door Developments Limited | Real estate | 2018 |
| Pipeland Laundry Services Ltd | Other services | 2019 |
| Pitcairn Property Management Limited | Hospitality | 2024 |
| Playfair Consultancy Group C.I.C. | Professional & scientific | 2014 |
| Playfair Estates Limited | Real estate | 2016 |
| Plumb 2 U Ltd. | Construction | 2003 |
| Pneumagen (Holdings) Limited | Professional & scientific | 2020 |
| Pneumagen Limited | Professional & scientific | 2015 |
| Poets' Neuk | Education | 2017 |
| Precedent P.D.R. Ltd. | Wholesale & retail | 2009 |
| Prestige Power Electrical Ltd | Construction | 2026 |
| Prime Caledonia Company Ltd | Real estate | 2024 |
| Promineon Ltd | Administrative & support | 2017 |
| Properly Resolutions Ltd | Information & communication | 2026 |
| PSC Property Ltd | Administrative & support | 2019 |
| PSV Studio LP | | 2015 |
| Quality Inspection GDG Ltd | Professional & scientific | 2026 |
| Questa Export LP | | 2015 |
| R & a Nominees Limited | Professional & scientific | 1993 |
| R H Taxis ST Andrews Limited | Transport & logistics | 2023 |
| R J Dunn Property Consultancy Limited | Real estate | 2010 |
| R&A Golf and Leisure Limited | Arts & recreation | 2009 |
| Radpix Limited | Administrative & support | 2020 |
| RAE & Raine Consultancy Ltd | Real estate | 2025 |
| Raith Taxis Limited | Transport & logistics | 2003 |
| Rastech Ltd | Professional & scientific | 2020 |
| Reactor X Ltd | Information & communication | 2024 |
| Realm Weave Ltd | Administrative & support | 2024 |
| Recharge Nutrition and Wellness Limited | Education | 2019 |
| Recovery and Breakdown Ltd | Transport & logistics | 2023 |
| Reith Properties Ltd | Real estate | 2020 |
| REM 777 Ltd | Mining & quarrying | 2024 |
| Resilire Ltd | Education | 2021 |
| Revills Ltd | Wholesale & retail | 2022 |
| Rewilding Kinkell Ltd | Agriculture & fishing | 2023 |
| RF Stonemasons Limited | Manufacturing | 2025 |
| Ribay Industries LP | | 2015 |
| Riverview Construction Ltd. | Administrative & support | 2015 |
| RJS Safety Consultants Ltd | Professional & scientific | 2016 |
| RNL Travel Ltd | Transport & logistics | 2022 |
| Roberts & Goring Ltd | Professional & scientific | 2025 |
| Robertson Estates and Properties Ltd | Education | 2013 |
| Rocca Continental Deli Limited | Wholesale & retail | 2017 |
| Rodan Limited | | 1987 |
| Rory COX Creative Ltd | Information & communication | 2024 |
| Roseangle House Limited | Real estate | 2014 |
| S3A Ltd | Hospitality | 2025 |
| Safeguarding Sensors Ltd | Manufacturing | 2026 |