| Edenside House Ltd | Hospitality | 2019 |
| Edward Barrett Limited | Real estate | 1976 |
| Elie Golf Limited | Wholesale & retail | 2022 |
| Elissen Ltd | Manufacturing | 2026 |
| Elite Edge Developments Ltd | Real estate | 2026 |
| Elite Golf Transport Ltd | Transport & logistics | 2018 |
| Elite Whisky Tours Ltd | Administrative & support | 2025 |
| Enhanced by Emily Ltd | Other services | 2024 |
| EOS Advisory LLP | | 2018 |
| Equine Herb Garden Limited | Hospitality | 2023 |
| Eriri Limited | Wholesale & retail | 2025 |
| Eris Intelligence Limited | Information & communication | 2024 |
| EST2000 Ltd | Professional & scientific | 2026 |
| Etera Ltd | Construction | 2011 |
| Event Bars ST Andrews Ltd | Hospitality | 2012 |
| Exvalve Limited | Manufacturing | 2024 |
| Fallout Ltd. | Hospitality | 2010 |
| Falside Mill Events Limited | Hospitality | 2020 |
| Faobhar Barber Ltd | Other services | 2026 |
| Farasafe Limited | Professional & scientific | 2024 |
| Fargotex LP | | 2015 |
| Ferass Trade LP | | 2015 |
| Fibreglass Collective Limited | Manufacturing | 2025 |
| Fielder AI Limited | Information & communication | 2019 |
| Fife Contemporary Art and Craft (ST. Andrews) Limited | Arts & recreation | 1988 |
| Fife Cottages Ltd | Real estate | 2012 |
| Fife Golf Trust | Arts & recreation | 2011 |
| Fife NDT Inspection Services Limited | Mining & quarrying | 2014 |
| Fife Wide Taxis Ltd | Transport & logistics | 2019 |
| Fifex Ltd | Professional & scientific | 2003 |
| Fifex [Scotland] Ltd | Manufacturing | 2018 |
| Fitpro Consultant Ltd | Administrative & support | 2025 |
| Flavour Match Making Limited | Information & communication | 2022 |
| Flawtoun LP | | 2015 |
| Fleetham Consulting Ltd | Professional & scientific | 2023 |
| Footmed Healthcare Services Limited | Health & social care | 2018 |
| Fordrose Limited | Professional & scientific | 1989 |
| Forevermore Pet Cremations Ltd | Other services | 2021 |
| Formartine BIO Limited | Professional & scientific | 2025 |
| Formlink Limited | Wholesale & retail | 1987 |
| Forth Connections Ltd | Other services | 2024 |
| Foster Offshore Limited | Mining & quarrying | 2018 |
| Fourwheel Leasing Ltd. | Financial services | 2003 |
| Fraise Consulting LP | | 2017 |
| Fraser Fyfe Limited | Real estate | 2012 |
| Fraser Fyfe Properties Limited | Real estate | 2014 |
| Full Circle Renovations (Scotland) Ltd | Construction | 2026 |
| Fyfe Mcdonald Limited | Professional & scientific | 2015 |
| Fyrish Bearings & Agricultural Supplies Ltd | Wholesale & retail | 2020 |
| G & K Limited | Construction | 1976 |
| G & M Scotia Ltd | Professional & scientific | 2021 |
| G ST News Limited | Wholesale & retail | 2016 |
| G&R Tech Traders Ltd | Wholesale & retail | 2026 |
| Ganesh Mobile Phones Traders Limited | Wholesale & retail | 2024 |
| Geffit Commerce LP | | 2015 |
| Gentle Dental Care Ltd | Health & social care | 2013 |
| Gentrylinks Books Ltd | Information & communication | 2025 |
| Geome Ltd | Professional & scientific | 2025 |
| George a Walker Ltd | Construction | 2011 |
| George a Walker Properties Ltd | Real estate | 2014 |
| GES OS Group Limited | Financial services | 2026 |
| Geum Limited | Construction | 1998 |
| Gibbins Property Edinburgh (Holdings) Limited | Real estate | 2016 |
| Gibbins Property Edinburgh Limited | Real estate | 2016 |
| Gibbs Taxis Ltd | Transport & logistics | 2017 |
| Glentress Bike Hire Ltd | Administrative & support | 2024 |
| Glenwood Salon Ltd | Other services | 2007 |
| Global Golf Ltd | Administrative & support | 2012 |
| Global Golfscapes ST Andrews Ltd | Professional & scientific | 2026 |
| GOK07 Ltd | Hospitality | 2022 |
| Golf International (Scotland) Limited | Administrative & support | 1995 |
| Golf Travel Design- ST Andrews Ltd | Administrative & support | 2025 |
| Golf Travellers Agency Limited | Administrative & support | 2008 |
| Gorgeous ST Andrews Ltd | Hospitality | 2014 |
| Grandiflora Market LP | | 2017 |
| Grange Trading Co (ST Andrews) Limited | Hospitality | 1977 |
| Green Cleen Fife Ltd | Administrative & support | 2025 |
| Guardbridge Bowling Club Limited | Other services | 2018 |
| Guardbridge Community Development Trust | Other services | 2017 |
| Guga Restaurants Ltd | Hospitality | 2012 |
| Haber Limited | Construction | 2016 |
| Hare Nest Accommodation Ltd | Hospitality | 2022 |
| Healy Aesthetics Ltd | Health & social care | 2022 |
| Heleborn LP | | 2015 |
| Henrys Little Limpet Ltd | Other services | 2013 |
| HHM1 Ltd | Hospitality | 2025 |
| Highfield Press Ltd | Information & communication | 2015 |
| Hillhead & Newton Farms Ltd | Agriculture & fishing | 2023 |
| Hnlsa Ltd | Other services | 2013 |
| Hollie Lawson Self Development Coach Ltd | Education | 2023 |
| Home-Start East Fife | Other services | 2000 |
| Hottappingonline.Com Ltd | Wholesale & retail | 2022 |
| House of Moir Limited | Construction | 2024 |
| Housework (Edinburgh) Ltd | Administrative & support | 2012 |
| HUA RAN Ltd. | Hospitality | 2013 |
| I & F Nassir Ltd | Hospitality | 2016 |
| I&M Property Management Ltd | Real estate | 2019 |
| Icaird Ltd | Extraterritorial | 2018 |
| Ident Business LP | | 2015 |
| IJD Ecom Ltd | Wholesale & retail | 2022 |