| 1 QT Development Limited | Construction | 2022 |
| 112 Advisory, ST Andrews Ltd | Arts & recreation | 2016 |
| 22A Events Ltd | Other services | 2019 |
| 400KV Saps Ltd | Energy | 2021 |
| 8 Golf Place ST Andrews Limited | Extraterritorial | 2026 |
| A & D Bar Services Ltd | Hospitality | 2010 |
| A & P Bearings Ltd. | Wholesale & retail | 2003 |
| A & P Turbine Services Limited | Manufacturing | 2009 |
| A & R Menzies Property Ltd | Construction | 2023 |
| A Mcqueen Engineering Ltd | Construction | 2013 |
| A2B Auto Centre Limited | Wholesale & retail | 2022 |
| Abigail MAY Golf Limited | Arts & recreation | 2023 |
| Actiframe Limited | Professional & scientific | 2021 |
| Actinoid Group of Companies Ltd | Financial services | 2020 |
| Adjusted Health Limited | Health & social care | 2016 |
| Adventures in Golf (UK) Limited | Administrative & support | 2007 |
| AES Healthcare Services Ltd | Health & social care | 2024 |
| Affectivehalo Ltd | Information & communication | 2023 |
| Afghanistan Center for the Study of Terrorism and Democracy (Acstd) Ltd | Professional & scientific | 2025 |
| African Lakes Company Limited | Professional & scientific | 2013 |
| African Lakes Philanthropy Ltd | Agriculture & fishing | 2005 |
| Aiqtec Ltd | Professional & scientific | 2014 |
| Ajbm Property Limited | Real estate | 2025 |
| Aksl Consultancy Ltd | Administrative & support | 2010 |
| Alba Residential (ST Andrews) Limited | Real estate | 2009 |
| Alex Thaddeus Hairdressing Ltd | Other services | 2013 |
| ALL Steps Building Advisor Ltd | Construction | 2020 |
| Alta Golf Events Limited | Administrative & support | 2015 |
| Alvega Trade LP | | 2015 |
| Anderson Advisory Ltd | Financial services | 2023 |
| Andrean Links Limited | Real estate | 2021 |
| Andrew Shanks IP Limited | Professional & scientific | 2018 |
| Angus & Perthshire Bearings Ltd. | Wholesale & retail | 2004 |
| Anticoin LP | | 2015 |
| AP Bearings & Agric. (Forfar) Ltd. | Wholesale & retail | 2008 |
| Apex Automotive 44 Ltd | Wholesale & retail | 2024 |
| Apolis Executive Limited | Professional & scientific | 2013 |
| Applied Business Research Ltd | Professional & scientific | 2024 |
| Aquila Space and Defence Technologies Ltd | Manufacturing | 2019 |
| ARC Fishings Limited | Agriculture & fishing | 2018 |
| Ardcraig Developments Limited | Real estate | 1997 |
| Argyle Court Owners Association Limited | Households | 2019 |
| Arrow Intercity L.P. | | 2009 |
| Art Quarter Fife Limited | Wholesale & retail | 2021 |
| Artery Gallery Limited | Wholesale & retail | 2004 |
| Ascendrix Systems Limited | Information & communication | 2025 |
| ASK the Angel Ltd | Information & communication | 2015 |
| Asteri Project LP | | 2015 |
| Auld Grey Toon Relocation Limited | Administrative & support | 2020 |
| Auld Reekie Media Limited | Information & communication | 2014 |
| Austen Gallery Limited | Wholesale & retail | 2019 |
| Avalon Business Park Ltd | Real estate | 2018 |
| Ayeorder Ltd | Information & communication | 2026 |
| Bacchus (ST Andrews) Ltd. | Wholesale & retail | 2003 |
| Badkat Apparel Ltd | Wholesale & retail | 2024 |
| Balkaithly Limited | Agriculture & fishing | 1998 |
| Balmullo.Net Limited | Information & communication | 2011 |
| Bangor Nails & Spa Limited | Other services | 2016 |
| BB Nutrition LP | | 2015 |
| BCM Consultancy Services Limited | Professional & scientific | 2018 |
| BE Rural Ltd | Professional & scientific | 2019 |
| Beaton Brothers Limited | Information & communication | 2008 |
| BEE Enterprises Ltd | Hospitality | 2012 |
| Beley Bridge Management Co Limited | Households | 2021 |
| Beley Farms Limited | Agriculture & fishing | 1955 |
| Benjamin Barker Barber and Shop Ltd | Other services | 2017 |
| Benjamin Barker Dundee Ltd | Other services | 2014 |
| Beslut Global LP | | 2015 |
| Bill Harkins Associates Ltd | Real estate | 2011 |
| Biodiversitees Ltd | Wholesale & retail | 2024 |
| Black Rock Holdings NO 6 Limited | Real estate | 2022 |
| Blebo Enterprises Limited | Information & communication | 2008 |
| Blebo Property Ventures Limited | Real estate | 2020 |
| Bloodhound Ltd | Professional & scientific | 2026 |
| Blue Star ST Andrews Ltd | Administrative & support | 2014 |
| Blush Consulting Limited | Professional & scientific | 2021 |
| BOB Millar Limited | Wholesale & retail | 1996 |
| Body & Mind Salon Ltd | Other services | 2010 |
| Bogart's PUB Ltd | Hospitality | 2017 |
| Braw Ventures Ltd | Information & communication | 2026 |
| Breo Ltd | Manufacturing | 2020 |
| Brinell Vision Ltd | Manufacturing | 2010 |
| Britta Commerce LP | | 2017 |
| Brownhills & Chesters Ltd | Agriculture & fishing | 2023 |
| Brownhills Garage Limited | Wholesale & retail | 2017 |
| Bryte Coffee Limited | Manufacturing | 2024 |
| Bubble UNI Life Limited | Information & communication | 2024 |
| Bulmar Commerce LP | | 2015 |
| Buzzqube Ltd | Administrative & support | 2023 |
| BV Siltint Ltd | Manufacturing | 2023 |
| Byre Youth Theatre Limited | Education | 2013 |
| C & a Autocentre Ltd | Wholesale & retail | 2009 |
| C & a Plant Services Ltd. | Construction | 2003 |
| C R Gordon Vehicle Sales Ltd | Wholesale & retail | 2011 |
| Caldon Ltd | Professional & scientific | 2009 |
| Caloride Limited | Real estate | 2011 |
| Camac 1001 Limited | Real estate | 2022 |
| Camac 1002 Limited | Construction | 2024 |
| Camac 1517 Limited | Real estate | 2022 |
| Camac Assets Limited | Real estate | 2022 |