| Ellway Group Ltd | Real estate | 2020 |
| Elves Quantity Surveying Ltd | Professional & scientific | 2023 |
| Elvora & Co Global Ltd | Wholesale & retail | 2025 |
| Empharm (North East) Limited | Wholesale & retail | 2013 |
| Encore Design Ltd | Wholesale & retail | 2021 |
| Energy Connection UK Limited | Professional & scientific | 2025 |
| Energy Recovery Limited | Water & waste | 2007 |
| Enhanced Aesthetics by Philippa LEE Limited | Health & social care | 2019 |
| EPD Building and Joinery Limited | Construction | 2024 |
| EPD Building Limited | Construction | 2024 |
| EPD Joinery Limited | Construction | 2024 |
| EPH Property Group Limited | Real estate | 2026 |
| Equine Answers Limited | Manufacturing | 2010 |
| Equine Massage Academy Limited | Other services | 2013 |
| Ersin Aydin Ltd | Wholesale & retail | 2025 |
| Estara Aesthetics & Wellness Ltd | Other services | 2025 |
| European Freight Forwarding Limited | Transport & logistics | 2015 |
| Evans Swinburn Limited | Professional & scientific | 2025 |
| Evolve Spinal Specialists Ltd | Health & social care | 2014 |
| Ewdo Ltd | Wholesale & retail | 2025 |
| Fair Teachers Ltd | Administrative & support | 2021 |
| Farmer the UK Ltd | Wholesale & retail | 2024 |
| Fast FRY Spennymoor Ltd | Hospitality | 2025 |
| Fastway Business Solutions Ltd | Administrative & support | 2017 |
| Fastway Properties Limited | Real estate | 2023 |
| Fathom Financial Holdings Limited | Financial services | 2026 |
| Fathom Financial Limited | Financial services | 2017 |
| Fayzer Ltd | Construction | 2024 |
| Feynes Ltd | Wholesale & retail | 2026 |
| Finadin Ltd | Information & communication | 2003 |
| Financial Dimensions (Partnership & Trust) LLP | | 2006 |
| Financial Dimensions U.K. Limited | Financial services | 2004 |
| Finnmark Limited | Wholesale & retail | 2016 |
| First Choice Bookkeeping & Financial Consultancy Ltd | Professional & scientific | 2021 |
| Fitzpatrick’S Food Limited | Hospitality | 2023 |
| Fletcher & Sons Haulage Ltd | Transport & logistics | 2023 |
| Fletchers of Spennymoor Limited | Other services | 2015 |
| Flexconsult Ltd | Professional & scientific | 2014 |
| Flusoul Ltd | Wholesale & retail | 2026 |
| Ford Rufus Limited | Real estate | 2023 |
| Forrest Productions Limited | Information & communication | 2019 |
| Fortisum Ltd | Wholesale & retail | 2026 |
| Foxcubbrewing Ltd | Manufacturing | 2025 |
| FR Professional Services Limited | Professional & scientific | 2023 |
| FR Training Ltd | Arts & recreation | 2019 |
| Frazer Home Group Ltd | Construction | 2025 |
| Fresh Accountants (Darlington) Limited | Professional & scientific | 2025 |
| Fresh Accountants Limited | Professional & scientific | 2024 |
| Fresh Business Group Limited | Professional & scientific | 2025 |
| Fresh Business Solutions Limited | Professional & scientific | 2018 |
| Fresh Business Utilities Limited | Energy | 2024 |
| Fresh Faced Aesthetics Ltd | Health & social care | 2017 |
| Fresh Peach Limited | Professional & scientific | 2024 |
| Fresh Point Properties Limited | Real estate | 2025 |
| Frigela Ltd | Wholesale & retail | 2026 |
| FSA Laundry & Ironing Ltd | Other services | 2023 |
| Funky Planet Ltd | Wholesale & retail | 2013 |
| Futureskills North East C.I.C. | Education | 2015 |
| G Mckenzie Groundwork's Limited | Construction | 2021 |
| G Smith Electrical Contractors Limited | Construction | 2018 |
| G&C Marketplace Ltd | Wholesale & retail | 2025 |
| G. D. O'Hehir & Co Limited | Professional & scientific | 2002 |
| Garen TI Ltd | Wholesale & retail | 2025 |
| GB1 Services Limited | Construction | 2015 |
| Gearnext Enterprises Limited | Wholesale & retail | 2026 |
| Geeks of the Galaxy Limited | Wholesale & retail | 2025 |
| Gemici Ltd | Wholesale & retail | 2026 |
| Genius Bidco Limited | Education | 2015 |
| Genius Holdco Limited | Education | 2015 |
| Getrekt Limited | Arts & recreation | 2018 |
| GGP Automotive Limited | Wholesale & retail | 2025 |
| GH Rentals Ltd | Real estate | 2026 |
| GJB Inspection Services Ltd | Professional & scientific | 2011 |
| Glassline Installations Limited | Construction | 2026 |
| Global Presence Limited | Professional & scientific | 2003 |
| Gonzopropertygroup Ltd | Real estate | 2025 |
| Goods Gillore Ltd | Wholesale & retail | 2024 |
| Gray Online Publishing Limited | Information & communication | 2024 |
| Green Durham CIC | Information & communication | 2021 |
| Grimmsorg Studios Ltd | Information & communication | 2017 |
| GROUP2 Fire Protection Limited | Professional & scientific | 2017 |
| Grow Education Limited | Education | 2019 |
| GSB Engineering NE Limited | Professional & scientific | 2005 |
| GSP March Construction Ltd | Construction | 2023 |
| Guava Estates Ltd | Real estate | 2018 |
| Guney Store Limited | Wholesale & retail | 2024 |
| H H GB Limited | Manufacturing | 2004 |
| Hadleys Haulage Ltd | Transport & logistics | 2019 |
| Harcon Design and Fabrication Limited | Manufacturing | 2024 |
| Hardinger Limited | Households | 2025 |
| Hasel Ltd | Wholesale & retail | 2025 |
| Hatchili Ltd | Wholesale & retail | 2025 |
| Hawk Shield Security Limited | Administrative & support | 2015 |
| Heart for the Garden C.I.C. | Education | 2021 |
| Heatwave Plumbing and Heating (NE) Limited | Construction | 2016 |
| Hedley Construction (North East) Limited | Construction | 2011 |
| Hedley Plant Limited | Administrative & support | 2024 |
| Helme Park Motor Services Limited | Wholesale & retail | 2009 |
| Hene Suntime Company Limited(The) | Households | 1983 |
| Henniker & Co Ltd | Professional & scientific | 2023 |