| THE Recovery Room Healthcare Ltd | Health & social care | 2026 |
| THE Red Beehive Company Ltd | Manufacturing | 2007 |
| THE Refiner's House International | Other services | 2013 |
| THE Renewal Path Ltd | Health & social care | 2025 |
| THE Respect System Limited | Professional & scientific | 2026 |
| THE Retriever PUB Company Limited | Hospitality | 2018 |
| THE RIB School Limited | Education | 2009 |
| THE RIG Shop Limited | Other services | 1989 |
| THE Roadie Kitchen Ltd | Hospitality | 2020 |
| THE Rockets Children's Motorcycle Display Team C.I.C. | Arts & recreation | 2018 |
| THE Rockingham Arms (West Wellow) Limited | Hospitality | 2026 |
| THE Rona Trust | Health & social care | 1998 |
| THE Roofing Division Limited | Construction | 2016 |
| THE Room Hairdressers Ltd | Other services | 2017 |
| THE Root of IT Limited | Education | 2013 |
| THE Rose Road Association | Health & social care | 1978 |
| THE Royal of Viceroy Limited | Wholesale & retail | 2020 |
| THE Rustic Events Company Ltd | Hospitality | 2019 |
| THE RYA Foundation | Arts & recreation | 2000 |
| THE SAB Partnership Limited | Professional & scientific | 2017 |
| THE Salmon Leap PUB Ltd | Hospitality | 2024 |
| THE Samosa Shop Ltd | Hospitality | 2010 |
| THE Samurai Garden Ltd | Hospitality | 2021 |
| THE Samurai Kitchen Ltd | Hospitality | 2019 |
| THE Sanctuary (Botley) Ltd | Other services | 2019 |
| THE Saucy Kitchen Ltd | Hospitality | 2025 |
| THE Saxon Calmore Limited | Hospitality | 2025 |
| THE Scoop Boutique Ltd | Hospitality | 2019 |
| THE Sea Miller Ltd | Information & communication | 2022 |
| THE Secret Viking Groovers Ltd | Arts & recreation | 2017 |
| THE Serendipity Centre Limited | Education | 2009 |
| THE Seriously Good Wine Company LLP | | 2013 |
| THE Services Manor Fishery Limited | Agriculture & fishing | 1995 |
| THE Seven Collective Ltd | Hospitality | 2026 |
| THE Sewing Studio Totton Limited | Other services | 2023 |
| THE Ship's Photographer (Services) Limited | Administrative & support | 1994 |
| THE Shoe Arcade Ltd | Professional & scientific | 2024 |
| THE Sholing Press Limited | Manufacturing | 2001 |
| THE Shuffle Group Limited | Hospitality | 2016 |
| THE Sign, Print and Clothing Company Ltd | Professional & scientific | 2018 |
| THE Silver Anchor Limited | Hospitality | 2017 |
| THE Sixsess Consultancy Ltd | Professional & scientific | 2022 |
| THE Skies the Limit Ltd | Manufacturing | 2024 |
| THE Sleepexchange Limited | Information & communication | 2019 |
| THE SM Family Ltd | Information & communication | 2024 |
| THE SO Spirit Co Ltd | Wholesale & retail | 2022 |
| THE SO Studio Limited | Manufacturing | 2017 |
| THE Soap Opera Cleaning Company Limited | Administrative & support | 2011 |
| THE Soap Opera Cleaning Master Company Ltd | Administrative & support | 2017 |
| THE Society of ST James | Health & social care | 1995 |
| THE Solent Boat Butler Limited | Manufacturing | 2022 |
| THE Solent Cluster Limited | Other services | 2023 |
| THE Solent Freeport Consortium Limited | Administrative & support | 2021 |
| THE Solent Octopus Project Ltd | Health & social care | 2024 |
| THE Solent Tech Group Limited | Information & communication | 2025 |
| THE Solid Surface Recycling Company Limited | Water & waste | 2024 |
| THE South Downs Coppice and Craft Centre Limited | Agriculture & fishing | 2014 |
| THE Southampton Collective CIC | Professional & scientific | 2016 |
| THE Southampton Police Club Trust Fund Limited | Financial services | 2002 |
| THE Southampton Sewing Hub CIC | Manufacturing | 2024 |
| THE Southampton Window Cleaner Ltd | Administrative & support | 2023 |
| THE Space Southampton Ltd | Other services | 2020 |
| THE Spaces Between Limited | Professional & scientific | 2020 |
| THE Spontaneous Travel Company Limited | Administrative & support | 2015 |
| THE Spray Foam Removal Specialist Ltd | Construction | 2025 |
| THE Spray Partnership Limited | Construction | 2008 |
| THE Springs Pilates Studio Ltd | Arts & recreation | 2022 |
| THE Square Southampton Limited | Construction | 2007 |
| THE Stables (Ampfield Hill) Management Limited | Households | 2018 |
| THE Stage Door Southampton Ltd | Hospitality | 2000 |
| THE Stams Photography Ltd | Information & communication | 2024 |
| THE Station Garage Service Centre Limited | Wholesale & retail | 2009 |
| THE Steakhouse @ Porters Ltd | Hospitality | 2023 |
| THE Stewart Brothers Limited | Extraterritorial | 2010 |
| THE Stickland PUB Company Ltd | Hospitality | 2020 |
| THE Study Spot Ltd | Education | 2025 |
| THE Sunnyfields Partnership Limited | Agriculture & fishing | 2003 |
| THE Superyacht Shop Limited | Wholesale & retail | 2018 |
| THE Surprise PUB Company Limited | Hospitality | 2020 |
| THE Swallows (Blocks Q, R, S and T) Management Company Limited | Households | 1996 |
| THE Swallows (Sholing) Residents Association Limited | Households | 1996 |
| THE Swan Inn Moreton Limited | Hospitality | 2009 |
| THE Swan Pool & Spa Centre Limited | Wholesale & retail | 2000 |
| THE Sweet Market Limited | Wholesale & retail | 2006 |
| THE Sycamores (Southampton) Management Company Limited | Households | 1989 |
| THE TAP House Ltd | Hospitality | 2014 |
| THE Teak Removals Company Limited | Manufacturing | 2023 |
| THE Theming Company Limited | Hospitality | 2012 |
| THE Thermometer Card Company Ltd | Wholesale & retail | 2024 |
| THE Thread Studio UK Ltd | Manufacturing | 2014 |
| THE TOW Bar Ltd | Wholesale & retail | 2024 |
| THE Towers Management Company (Southampton) Limited | Households | 1995 |
| THE Trades Van Centre Ltd | Wholesale & retail | 2016 |
| THE Trauma Clinic Ltd | Health & social care | 2026 |
| THE Travel Pundit Ltd | Administrative & support | 2019 |
| THE Truprint Group | Information & communication | 2018 |
| THE Trusty Servant Limited | Hospitality | 2018 |
| THE Turnbull Group Limited | Information & communication | 2017 |
| THE Turning Point Project Ltd | Health & social care | 2008 |
| THE Twenty SIX Collective Ltd | Professional & scientific | 2024 |