| Riverside Electrical Control Services Limited | Manufacturing | 2004 |
| RNG VKY Limited | Wholesale & retail | 2025 |
| Robertson & Best Developments Limited | Real estate | 2025 |
| Robson Estate Management Limited | Real estate | 2007 |
| ROD Horwell Limited | Administrative & support | 2003 |
| Roomsearch Limited | Administrative & support | 2005 |
| Rossington Tools Limited | Wholesale & retail | 2007 |
| Rotherham Tool Hire Limited | Administrative & support | 1997 |
| Round & Graham Limited | | 1961 |
| Roundabout Limited | Health & social care | 1997 |
| Rowan Electrical Communications Limited | Construction | 1998 |
| RTL Plumbing & Heating Ltd | Construction | 2006 |
| Russian Language Services Limited | Administrative & support | 2005 |
| Rutland Autobodies Limited | Wholesale & retail | 2006 |
| Rutland Cutlery Co.Limited | Manufacturing | 1970 |
| Rverd Inc. Ltd | Hospitality | 2026 |
| S 2 Squared Limited | Professional & scientific | 2007 |
| S L Joinery Limited | Construction | 2007 |
| S S Insulations Limited | Wholesale & retail | 2005 |
| S-Thaya Ltd | Wholesale & retail | 2019 |
| S. V. Timber Ltd | Wholesale & retail | 2004 |
| S.A. Highfield Property Management (Doncaster) Limited | Real estate | 1994 |
| S.A. Ibbotson Limited | Real estate | 2003 |
| S.E.M.S. Limited | Construction | 2001 |
| SA37 Limited | Real estate | 2017 |
| SAB Bros Limited | Information & communication | 2026 |
| Saharan Trading Company Limited | Health & social care | 1964 |
| Salary Solutions Limited | Administrative & support | 2003 |
| Salomon Engineering Limited | Manufacturing | 2002 |
| Salt Trading Limited | Hospitality | 2004 |
| SAN & Amad General Trade Company Ltd | Wholesale & retail | 2015 |
| Sandygate Property Limited | Households | 2000 |
| Sapphire Electrical and Communications Limited | Information & communication | 1997 |
| Saville ROW Developments Limited | Construction | 2004 |
| Sawblast Services Limited | Manufacturing | 2003 |
| Scholes Financial Management Limited | Financial services | 2007 |
| Script Media Group Ltd | Information & communication | 1897 |
| Seaman Herbert & Co Limited | Professional & scientific | 2002 |
| Seatax Limited | Professional & scientific | 1979 |
| Serialpoint Limited | Construction | 1994 |
| Servalift Limited | Wholesale & retail | 1998 |
| Servicrest Limited | | 1978 |
| Servoscript (Laboratory Recorders) Limited | | 1982 |
| Seven Ports Limited | Real estate | 2003 |
| Shafiq Mart Ltd | Wholesale & retail | 2025 |
| Shania Limited | Wholesale & retail | 2007 |
| Sharp Consultancy Limited | Administrative & support | 1999 |
| Shaw Motors Limited | Wholesale & retail | 2003 |
| Sheaf Brickwork Limited | Construction | 2000 |
| Shearstone Mechanical Ltd | Professional & scientific | 2008 |
| Sheer Challenge Limited | Construction | 2000 |
| Sheffield Art Metal Limited | Manufacturing | 1982 |
| Sheffield Catering Supplies Limited | Wholesale & retail | 2003 |
| Sheffield Futures | Education | 1994 |
| Sheffield Physiotherapy Ltd | Health & social care | 2007 |
| Sheffield Pneumatic Services Limited | Professional & scientific | 2003 |
| Sheffield Science Park Company Limited | Real estate | 1984 |
| Sheffield Security Supplies Limited | Professional & scientific | 1978 |
| Sheffield Sports Stadium Limited | Administrative & support | 1961 |
| Sheffield Theatres Crucible Trust | Arts & recreation | 2006 |
| Sheffield Transport Sports Club Limited | Arts & recreation | 1991 |
| Sheffield Venetian Blind Co. Limited | Administrative & support | 2003 |
| Sheffield Vision Limited | Information & communication | 2001 |
| Shop-At-Home Limited | Extraterritorial | 2000 |
| SID Miles Limited | | 1951 |
| Silkstone Investments Limited | Extraterritorial | 1989 |
| Silver Service Consultancy Limited | Professional & scientific | 2006 |
| Silverark Limited | Wholesale & retail | 2007 |
| Simon Producer Ltd | Information & communication | 2005 |
| Sintra Developments Limited | Construction | 2005 |
| Sister UK Limited | Wholesale & retail | 2004 |
| Siwis Limited | Information & communication | 2005 |
| Skills Choice (SC) | Administrative & support | 2001 |
| Slaters Hair Care Limited | Other services | 2002 |
| Snowflake Animal Bedding Ltd | Water & waste | 2005 |
| Social Housing Solutions Limited | Wholesale & retail | 2004 |
| Solarvista Software Limited | Information & communication | 1990 |
| Sothall Roofing Specialists Limited | Construction | 2004 |
| Source the Course Limited | Professional & scientific | 2005 |
| South Yorkshire Tile Company Limited | Professional & scientific | 1994 |
| South Yorkshire Trades Historical Trust Limited | Arts & recreation | 2002 |
| Southgrove Lodge Management Limited | Households | 1992 |
| Space Studio Limited | Professional & scientific | 2005 |
| Spatial Mechanical Services Ltd | Construction | 2007 |
| SPC Internet Ltd | Wholesale & retail | 1983 |
| Special Machined Products Limited | Manufacturing | 1986 |
| Special Steel Co.,Limited | Manufacturing | 1925 |
| Speed Frame (Northern) Limited | | 2002 |
| Speedform (Holdings) Limited | Construction | 1969 |
| Spotlight Productions (UK) Ltd | Information & communication | 2007 |
| Sprayframe Limited | Wholesale & retail | 2005 |
| Springfield NU Build Limited | Construction | 2018 |
| Springvale Flats Owners Association Limited | Real estate | 2006 |
| Springwell Court Management Company Limited | Households | 1982 |
| Square Pebble Limited | Construction | 2006 |
| ST Joseph's Day Nursery & Pre-School Ltd | Education | 2002 |
| ST. Luke's Promotions Limited | Administrative & support | 1994 |
| Stainless Plating Limited | Manufacturing | 1926 |
| Stairlift Solutions Limited | Wholesale & retail | 2002 |
| Stan Plus Stan Two Limited | Professional & scientific | 2000 |