| 00191802 Limited | Manufacturing | 1923 |
| 00697522 Limited | Manufacturing | 1961 |
| 2 Sorella's Limited | Other services | 2007 |
| 416 Property Management Limited | Households | 1991 |
| 911 Auto Repairs Limited | Wholesale & retail | 2005 |
| A & H Builders Ltd | Construction | 2003 |
| A & J Real Estate 2021 Ltd. | Real estate | 2005 |
| A & MPG Limited | Professional & scientific | 2003 |
| A B T Enterprises Ltd | Wholesale & retail | 1997 |
| A H PIX Limited | Professional & scientific | 2005 |
| A J D Roofing Limited | Construction | 2002 |
| A L Simpkin (Group) Limited | Professional & scientific | 2000 |
| A-Print (Chesterfield) Limited | Manufacturing | 1997 |
| A.G.S. Commercials & Cars (Doncaster) Limited | | 1979 |
| A.L.T. Accountants Limited | Professional & scientific | 2003 |
| Abbey Forged Products Limited | Manufacturing | 1982 |
| Abbeydale Brewery Limited | Manufacturing | 2002 |
| Abbeydale DIY Limited | Wholesale & retail | 2006 |
| Abco Seals Limited | Manufacturing | 1989 |
| ABD Enterprises Ltd | Hospitality | 2004 |
| Abeemart Limited | Wholesale & retail | 2024 |
| Abrish Store Limited | Wholesale & retail | 2025 |
| Ache Hardware Ltd | Wholesale & retail | 2025 |
| Acredula Group Limited | Information & communication | 1982 |
| Active Faith Limited | Education | 2004 |
| Actual Strategies Limited | Information & communication | 2003 |
| Adrian Evans Limited | Construction | 2006 |
| Ahmtax Limited | Professional & scientific | 2007 |
| Airflow Environmental Limited | Manufacturing | 2000 |
| Airflow World Group Limited | Manufacturing | 2000 |
| Airmaster (Heating & Ventilation) Limited | Construction | 1998 |
| Akbar Travel & Tours Ltd | Administrative & support | 2003 |
| Albany Mechanical Services Limited | Wholesale & retail | 1978 |
| Albert Jackson (Domestic Equipment) Limited | Professional & scientific | 1960 |
| Albert Marsh and Bestelite Hardfacing Co Limited | Manufacturing | 1945 |
| Allan Morton Butchers of Distinction Limited | Wholesale & retail | 2005 |
| Allfont Limited | Manufacturing | 2003 |
| Allinblay Properties Limited | Real estate | 1998 |
| Aluminium & Glazing Services Limited | Professional & scientific | 2007 |
| Amar Beauty Ltd | Other services | 2007 |
| American Professional 1 Limited | Other services | 2006 |
| AMP Contracts Ltd. | Wholesale & retail | 2006 |
| Amtec CNC Ltd | Construction | 2006 |
| Andrew Smith (Construction) Limited | Wholesale & retail | 1974 |
| Andy Gallacher Photography Limited | Arts & recreation | 2003 |
| Antiquity Marble Limited | Manufacturing | 1990 |
| Applied Programming Consultancy Limited | Information & communication | 1995 |
| Aquilonis Developments Ltd | Real estate | 2011 |
| Arden Winch & Co Limited | Wholesale & retail | 1976 |
| Ariel & Angel PUB Co Limited | Hospitality | 2025 |
| Arkhive Limited | Hospitality | 1999 |
| Arms Park Developments Ltd. | Professional & scientific | 1992 |
| Artisan Stone Ltd | Manufacturing | 2005 |
| Ashworth Bailey Limited | Professional & scientific | 2003 |
| Assetwell Limited | Wholesale & retail | 1983 |
| Associated GAS Services (UK) Ltd | Construction | 2004 |
| Assured Consultancy Services Ltd | Professional & scientific | 2025 |
| Astarian Enterprises Limited | Transport & logistics | 2006 |
| Aston Investments Limited | Real estate | 1956 |
| Atena Holding Ltd | Financial services | 2025 |
| Athersley Community Association and Football Club | Arts & recreation | 2002 |
| Attercliffe Tyres Limited | Wholesale & retail | 2001 |
| Audio Images Limited | Wholesale & retail | 1996 |
| Autoleisure Limited | Professional & scientific | 1981 |
| Automotion CPM Group Limited | Wholesale & retail | 2007 |
| B.A. Haxby (Barnsley) Limited | Manufacturing | 1984 |
| B.P. Dempsey Limited | Construction | 1957 |
| Badgerfields (Management) Limited | Households | 2004 |
| Balaman Properties Limited | Real estate | 2002 |
| Banner Cross Hall Flats Limited | Households | 1971 |
| Bar 24 Limited | Construction | 2001 |
| Barber Design Limited | Professional & scientific | 2007 |
| Barkers the Bakers Limited | Manufacturing | 2000 |
| Barlows Bespoke Interiors Limited | Construction | 2025 |
| Barnsley FC Community Trust Limited | Arts & recreation | 2007 |
| Barnsley Football Club Limited | Arts & recreation | 2002 |
| Barnsley Muslim Community Centre Ltd | Other services | 1996 |
| Barnsley Riding for the Disabled Association | Arts & recreation | 2006 |
| Barratt Roofing Limited | Construction | 2003 |
| Barton Homes Ltd | Real estate | 2001 |
| Barton Real Estates Limited | Real estate | 2000 |
| Basbito ET EL Ltd | Extraterritorial | 2007 |
| Bawtry Road Service Station Limited | Wholesale & retail | 1975 |
| BCB Trading Limited | Construction | 2001 |
| Beacon South Yorkshire Ltd. | Health & social care | 2005 |
| Beckett and Wood (Builders) Limited | Wholesale & retail | 1937 |
| Beechwood (Rotherham) Management Limited | Households | 1984 |
| Belle VUE Property Limited | Real estate | 2000 |
| Belton Property Developers Limited | Construction | 2004 |
| Benditnow Limited | Manufacturing | 2007 |
| Bennett's Motorcycles Limited | Wholesale & retail | 1997 |
| Bentley Road Veterinary Practice Limited | Professional & scientific | 2008 |
| BH & C AG Ltd | Agriculture & fishing | 2024 |
| Birdline Auto Services Limited | Wholesale & retail | 2002 |
| Blake Aerials Limited | Extraterritorial | 2002 |
| Blast-Parts-Media (BPM) Ltd | Professional & scientific | 2001 |
| Bloc Projects Ltd | Arts & recreation | 2007 |
| BMK Property NW Ltd | Real estate | 2021 |
| Bolehill Mews Management Company Ltd | Households | 2000 |
| Bonapartes (Leisure) Limited | Extraterritorial | 1986 |