| Aldium Insurance Services Ltd | Financial services | 1996 |
| Alpha (IOM) Ltd | | 1983 |
| Batmel LLP | | 2011 |
| Brentcove Limited | Construction | 1991 |
| Browsers Emporium Ltd | Wholesale & retail | 2016 |
| Bsac International Limited | Arts & recreation | 1986 |
| Concept Interiors Neston Limited | Wholesale & retail | 1992 |
| Crilly & Burns Building Contractors Limited | Construction | 2005 |
| Critical Skills Development Limited | Professional & scientific | 1989 |
| Darlingtons (Group) Limited | Wholesale & retail | 1942 |
| Forward Builders Supplies Limited | Wholesale & retail | 1987 |
| Glowquest Limited | Wholesale & retail | 1987 |
| Great Sutton Garage Northwest Limited | Wholesale & retail | 2002 |
| Greif UK Holding Ltd | Professional & scientific | 2008 |
| Greif UK Ltd | Manufacturing | 2008 |
| GUY Payne & Co (Accounting Services) Limited | Professional & scientific | 1995 |
| Hamperfection Limited | Wholesale & retail | 1986 |
| Highway Emulsions Limited | Professional & scientific | 1991 |
| Iaab Limited | Construction | 2013 |
| J.Darlington (Heswall) Limited | Professional & scientific | 1951 |
| Multistream Limited | Professional & scientific | 1986 |
| Nynas Bitumen Limited | Professional & scientific | 1970 |
| Nynas Limited | Professional & scientific | 1989 |
| Origintrade Limited | Professional & scientific | 2000 |
| P&A Benbow Ltd | Construction | 2005 |
| Phillip Bates & Co Financial Services Limited | Financial services | 1998 |
| Principal Bldg Supplies Ltd | Wholesale & retail | 1998 |
| Pro Pipe Engineering Limited | Construction | 2009 |
| Profile Deflashing Compounds Limited | Manufacturing | 1993 |
| Promatic Holdings Limited | Manufacturing | 2014 |
| Rossendale Group Limited | Manufacturing | 1949 |
| SGS Cambridge Limited | Professional & scientific | 1990 |
| SGS Holding UK Ltd | Professional & scientific | 1998 |
| SGS United Kingdom Limited | Professional & scientific | 1974 |
| Sutton Glass Ltd | Construction | 2022 |
| Two Mills Chester Ltd | Hospitality | 2024 |
| Vanguard IVS | | 1997 |
| Well CUT Limited | Other services | 2017 |
| Wexmay Engineering Contractors Limited | | 1977 |
| Whitrovian Limited | Real estate | 1971 |