| Accelerate Technologies Limited | Information & communication | 2018 |
| Aduwa Limited | Professional & scientific | 2023 |
| Babyease Limited | Administrative & support | 2024 |
| Better Journals Ltd | Wholesale & retail | 2026 |
| Bioc Ltd | Professional & scientific | 2010 |
| BNG South Limited | Professional & scientific | 2025 |
| Bradley's Lake Management Company Limited | Households | 2023 |
| Cotswold Lakes Holiday Lodges Ltd | Hospitality | 2020 |
| Cotswold Lakeside Lodges Ltd | Hospitality | 2020 |
| Dave Cobb Transport Limited | Transport & logistics | 2006 |
| Dominic Winter (Auctioneers) Ltd | Administrative & support | 1998 |
| Edwards College Management Company Limited | Professional & scientific | 1978 |
| Facades Design and Management Limited | Professional & scientific | 2018 |
| Felanicci Development Ltd | Real estate | 2022 |
| Flowerberry Limited | | 1979 |
| Greystone Developments UK Limited | Construction | 2006 |
| Health Insurance Compare Ltd | Financial services | 2019 |
| Hercules Real Estate Holdings Limited | Financial services | 2025 |
| Holistay UK Ltd | Hospitality | 2020 |
| Keep Calm Finance Limited | Hospitality | 2018 |
| Kingfisher Place Financial Planning Ltd | Financial services | 2024 |
| Lake 11 LLP | | 2006 |
| Lanson Holdings Limited | Manufacturing | 2023 |
| Lanson Polymers Limited | Manufacturing | 2010 |
| Lanson Solutions Limited | Real estate | 2025 |
| M Warman Contractors Limited | Construction | 2020 |
| Michael LEE Limited | Construction | 2023 |
| Motion Marketing Limited | Administrative & support | 2017 |
| Orion UK Holdings Limited | Professional & scientific | 2023 |
| Pace Networks Limited | Energy | 2011 |
| Staycation Cotswold Lakes Ltd | Hospitality | 2020 |
| Summer Lake Management Company Limited | Households | 2023 |
| Supportwise IT Services Ltd. | Information & communication | 2019 |
| T&M Landscaping and Driveways Limited | Administrative & support | 2025 |
| Tektology Holdings Limited | Information & communication | 2020 |
| Trufflehunter Ltd | Manufacturing | 2007 |
| Tyto Designs Ltd | Manufacturing | 2023 |
| Watermark Land Company Limited | Professional & scientific | 2011 |