| DR Nicholas Todd Ltd | Health & social care | 2015 |
| DR S D Robertson Ltd | Information & communication | 2022 |
| DR S Khan Limited | Health & social care | 2012 |
| Drain Surv Limited | Construction | 2025 |
| Drake Lane Associates Ltd. | Professional & scientific | 1999 |
| Draughton Heights Ltd | Hospitality | 2025 |
| Dream Golf Holidays Ltd | Administrative & support | 2012 |
| Driver Supply Ltd | Administrative & support | 2013 |
| DSM Products Limited | Manufacturing | 2023 |
| Duncan Taylor Associates Limited | Professional & scientific | 2014 |
| DVD Holdings Ltd. | Administrative & support | 2017 |
| DWL Marketing Consultancy Ltd | Professional & scientific | 2016 |
| Dykewerk Ltd | Information & communication | 2026 |
| Dynamic Tool Devices Ltd | Wholesale & retail | 2014 |
| Dyson Engineering Group Limited | Financial services | 2026 |
| Dyson Engineering Holdings Ltd | Professional & scientific | 2021 |
| Dyson Engineering Ltd | Manufacturing | 2000 |
| E a Gibbs Plumbers Limited | Construction | 2023 |
| Early Doors Micro Bar Limited | Hospitality | 2024 |
| Early Start Private Day Nursery Limited | Education | 2017 |
| Earth to Earth Organics Ltd | Manufacturing | 2020 |
| Eartha Life Ltd | Wholesale & retail | 2023 |
| Earthwise Rewards Ltd | Information & communication | 2024 |
| East Leigh Dental Care Ltd | Health & social care | 2012 |
| East Midlands Commercial Limited | Wholesale & retail | 2023 |
| Eastrain Limited | Extraterritorial | 2020 |
| Ebor Fabrics Limited | Wholesale & retail | 1981 |
| Edattel Healthcare Ltd | Health & social care | 2014 |
| Eddie B Webster & Sons Ltd | Transport & logistics | 2006 |
| Eddy’S Toilet Company Ltd | Administrative & support | 2025 |
| Edinburgh School of Music Limited | Arts & recreation | 2017 |
| Education Works Limited | Education | 2009 |
| Educational Grant Solutions Limited | Administrative & support | 2021 |
| Efixx Ltd | Administrative & support | 2021 |
| Efleety Limited | Information & communication | 2024 |
| EJW Limited | Professional & scientific | 2024 |
| Elben Properties Limited | Hospitality | 2016 |
| Elcock Tools Ltd | Wholesale & retail | 2025 |
| Elderbeck Limited | Hospitality | 2004 |
| Eleconline Limited | Extraterritorial | 2009 |
| Elegant Finishes Limited | Construction | 2024 |
| Elements M&E Ltd | Construction | 2021 |
| Elite 100 Cars Limited | Financial services | 2015 |
| Elite Property Services Northern Limited | Construction | 2007 |
| Elizabeth Ellis Limited | Financial services | 2024 |
| Elkie Limited | Real estate | 2018 |
| Ellergill Barristers Limited | Professional & scientific | 2016 |
| Ellergill Holiday Lettings LLP | | 2016 |
| Ellis Clark Trains Holding Co Ltd | Financial services | 2025 |
| Ellis Clark Trains Ltd | Manufacturing | 2017 |
| Ellison Civils Limited | Construction | 2013 |
| Ellison Construction Limited | Construction | 2005 |
| Ellison Construction Projects Limited | Construction | 2014 |
| Ellison Events Limited | Other services | 2023 |
| Ellison Group Holdings Limited | Financial services | 2015 |
| Ellison Homes Ltd | Real estate | 2020 |
| Ellison Scaffolding Ltd | Construction | 2025 |
| Elsworth at the Mill Ltd | Hospitality | 2022 |
| Elsworth Kitchen Limited | Hospitality | 2018 |
| Elvaston Investments Limited | Real estate | 1982 |
| Elvaston Properties Limited | Real estate | 1997 |
| Embodied Growth Ltd | Health & social care | 2025 |
| Embrace & Evolve Marketing Limited | Professional & scientific | 2025 |
| Emigration IS the Only Way Limited | Extraterritorial | 2019 |
| Emma Williams Ltd | Professional & scientific | 2021 |
| Empowerag Ltd | Professional & scientific | 2025 |
| Empowering Performance Limited | Professional & scientific | 2021 |
| EMS Ambulance Ltd | Professional & scientific | 2010 |
| EMT Marine Limited | Administrative & support | 2025 |
| Energyflowe Limited | Administrative & support | 2017 |
| EPB Services Limited | Information & communication | 2005 |
| EPB Ventures Limited | Professional & scientific | 2015 |
| Equiculture Ltd | Education | 2017 |
| ERW Construction Limited | Professional & scientific | 2009 |
| ERW Investments Yorkshire Ltd | Other services | 2025 |
| Escalator Solutions Ltd | Wholesale & retail | 2022 |
| Eshton Estates LLP | | 2008 |
| Eshton Heritage Developments Limited | Construction | 2022 |
| Eshton Park Limited | Agriculture & fishing | 2023 |
| Esja Investments Limited | Extraterritorial | 2023 |
| Ethicare Housing Limited | Real estate | 2026 |
| Euroblast Limited | Extraterritorial | 1974 |
| Euroventure Ltd | Wholesale & retail | 2025 |
| Euroventure Transport Ltd | Administrative & support | 2017 |
| Euroventure Travel Ltd | Administrative & support | 2012 |
| EVE Products Limited | Manufacturing | 2002 |
| Event Finder Ltd | Administrative & support | 2024 |
| Event Fire Services Limited | Health & social care | 2014 |
| Event Medical Services Ltd | Health & social care | 2010 |
| Evergreen Plastic Products Limited | Manufacturing | 2025 |
| Evolve Business Consulting Limited | Professional & scientific | 2015 |
| Exceptive Limited | Professional & scientific | 2008 |
| Exclusive Commercial Services Limited | Financial services | 2022 |
| Exclusive Mortgage Advice Services Limited | Financial services | 2019 |
| Exhibitionstore.Com Limited | Wholesale & retail | 2008 |
| F & F Construction and SON Limited | Wholesale & retail | 2006 |
| F Brewer & SON Limited | Agriculture & fishing | 2014 |
| F&D Bistro Ltd | Hospitality | 2025 |
| Fabrikate Ltd | Information & communication | 2014 |
| Falcon Inn Arncliffe Ltd | Hospitality | 2019 |