| DTD Express Ltd | Transport & logistics | 2020 |
| Dtsi Limited | Real estate | 2026 |
| Dudda's TUN Ltd | Manufacturing | 2013 |
| Dunbar Wharf Holdings Limited | Professional & scientific | 2000 |
| Dunbar Wharf Limited | Professional & scientific | 1923 |
| Dungeons & Dice Ltd | Wholesale & retail | 2026 |
| Dunsfold Associates Limited | Professional & scientific | 2013 |
| Dutch Pancakes Limited | Hospitality | 2014 |
| Dylanmichael Holdings Ltd | Financial services | 2024 |
| Dynamic Cleaning & Services Ltd | Administrative & support | 2019 |
| Dynamic Doors Ltd | Administrative & support | 2016 |
| DYS Leisure Limited | Hospitality | 2006 |
| Dziwa Limited | Health & social care | 2020 |
| E E L IT Ltd | Information & communication | 2004 |
| E&L Aquatics Ltd | Wholesale & retail | 2025 |
| E.C. Construction Group Ltd | Construction | 2025 |
| E.H. Nicholls Holdings Limited | Construction | 2000 |
| E.H. Nicholls, JNR. Limited | Transport & logistics | 1967 |
| E.P.S. Logistics Technology Limited | Manufacturing | 1977 |
| E.W. Taylor & Company (Forwarding) Limited | Transport & logistics | 1960 |
| Eade Court RTM Company Limited | Households | 2026 |
| Eamo Ltd | Transport & logistics | 2025 |
| East Charcoal Grill Ltd | Hospitality | 2024 |
| East END Holdings Ltd | Other services | 1997 |
| East Kent Rentals Limited | Administrative & support | 2015 |
| East Milton Transport Ltd | Transport & logistics | 2014 |
| East Star Horizons Ltd | Education | 2025 |
| East Sussex Medical Services Limited | Health & social care | 2019 |
| Eastchurch Construction Ltd | Construction | 2020 |
| Easterhay Limited | Professional & scientific | 2002 |
| Eastwood Access Ltd | Construction | 2026 |
| Easy EX Ltd | Financial services | 2019 |
| Eave & Apex Limited | Administrative & support | 2015 |
| EBD Trading Ltd | Wholesale & retail | 2026 |
| EBP Holdings Limited | Financial services | 2021 |
| EBP Mitchell Limited | Financial services | 2022 |
| Eccho Design and Consultancy Limited | Professional & scientific | 2024 |
| Echo and Frame Limited | Professional & scientific | 2026 |
| Echo House Management Company Limited | Households | 1993 |
| Echo Papa Developments Limited | Information & communication | 2019 |
| Eckman Limited | Wholesale & retail | 2014 |
| Eclipse Insights Ltd | Information & communication | 2020 |
| Ecoffins Ltd | Manufacturing | 1995 |
| Ecoffins UK LLP | | 2012 |
| Ecomagic Ltd | Extraterritorial | 2005 |
| Ecosan Services Limited | Water & waste | 2007 |
| Ecospark Solar Solutions Ltd | Manufacturing | 2025 |
| Eddiecon Ltd | Construction | 2015 |
| Eden (Downlands) Limited | Construction | 2018 |
| Eden (Thorpe) Limited | Construction | 2017 |
| Eden (Thorpe) Two Limited | Construction | 2024 |
| Eden Projects One LLP | | 2019 |
| Eden Retirement Living Ltd | Construction | 2016 |
| Eden Retirement Projects Ltd | Construction | 2019 |
| Edesa Ltd | Wholesale & retail | 2019 |
| Edge Compliant Security Solutions Ltd | Administrative & support | 2026 |
| Edgegate Ltd | Information & communication | 2025 |
| EDK Trading Group Ltd | Wholesale & retail | 2021 |
| Educrush Games Ltd | Manufacturing | 2025 |
| Edunne Healthcare Ltd | Wholesale & retail | 2022 |
| Edutrans Limited | Administrative & support | 2017 |
| EDW Accounting and Bookkeeping Ltd | Professional & scientific | 2021 |
| Edward House Twenty-Twenty Limited | Households | 2020 |
| Edward Wheatcroft Limited | Professional & scientific | 2015 |
| Edwards Heating (Kent) Limited | Construction | 2012 |
| EED Business Limited | Hospitality | 2020 |
| Effortless Stays Limited | Hospitality | 2026 |
| EGG Plant Hire Ltd | Administrative & support | 2026 |
| Egginton Ford Consulting Limited | Professional & scientific | 2026 |
| Eglinton Holdings Limited | Real estate | 2026 |
| Egowao Limited | Construction | 2021 |
| EHZ Consulting Ltd | Financial services | 2024 |
| Eight Two Studio Ltd | Information & communication | 2022 |
| EJ Brickwork Ltd | Construction | 2024 |
| EJ Computing Ltd | Wholesale & retail | 2026 |
| Ejn's Property Investments Ltd | Administrative & support | 2006 |
| EKA Technologies Ltd | Information & communication | 2026 |
| EKE Projects Ltd | Construction | 2024 |
| EL Manny Limited | Construction | 2025 |
| El-Menif GLO Ltd | Wholesale & retail | 2026 |
| Elbrus Construction Ltd | Construction | 2016 |
| ELE Harris Holdings Ltd. | Other services | 2022 |
| Electraweld Limited | Wholesale & retail | 1991 |
| Electrical Empire Limited | Construction | 2013 |
| Electrical Lighting Supplies (Dartford) Limited | Administrative & support | 2024 |
| Electrical Lighting Supplies (Maidstone) Limited | Administrative & support | 2023 |
| Electrical Lighting Supplies (Sevenoaks) Limited | Administrative & support | 2023 |
| Electrical Lighting Supplies (Sittingbourne) Limited | Administrative & support | 2023 |
| Electrical Lighting Supplies Limited | Administrative & support | 2014 |
| Eleosconsulting Ltd | Administrative & support | 2025 |
| Elephant Roots Bookkeeping Limited | Professional & scientific | 2025 |
| Elespa Limited | Manufacturing | 1996 |
| Elevate Childcare Limited | Health & social care | 2025 |
| Elevator Survey & Design Ltd | Construction | 2011 |
| Elex Solutions Ltd | Construction | 2026 |
| Elifcan Cafe Limited | Hospitality | 2017 |
| Elijah & Charles Global Logistics Ltd | Transport & logistics | 2026 |
| Elika Properties Ltd | Real estate | 2020 |
| Elite Appointments Limited | Administrative & support | 2017 |
| Elite Building Restoration Limited | Construction | 2024 |