| Electus Energy Ltd | Construction | 2022 |
| Elevate Corporation Ltd | Information & communication | 2013 |
| Elevate Fitness Worthing Limited | Arts & recreation | 2024 |
| Elevate Lead Group Ltd | Construction | 2026 |
| Eleven Eleven Jewellery Ltd | Manufacturing | 2024 |
| Elite Designers Limited | Construction | 2000 |
| Elite Paint Spray Solutions Limited | Construction | 2024 |
| Elsa Systems Limited | Administrative & support | 2008 |
| Elumanati Ltd | Information & communication | 2025 |
| EMC Contractors Ltd | Construction | 2022 |
| Emerald Quay Consultancy Ltd | Information & communication | 2025 |
| Emily Button Coaching Ltd | Professional & scientific | 2025 |
| Emjay Holdings Ltd | Real estate | 2021 |
| Emjay Media Limited | Information & communication | 2008 |
| Empire Land and Homes Limited | Construction | 2017 |
| Empire Smart Installations Limited | Construction | 2020 |
| Empowered Human Design Ltd | Extraterritorial | 2022 |
| Empowering Minds Ltd. | Health & social care | 2014 |
| Endeavour Holdings Limited | Real estate | 1920 |
| Enterprise for Growth Ltd | Professional & scientific | 2014 |
| Entropie Limited | Information & communication | 2004 |
| Enviroblinds Ltd | Construction | 2012 |
| Environmental Safety Ltd | Professional & scientific | 2022 |
| Eoid Ltd | Information & communication | 2013 |
| Epower Projects Limited | Extraterritorial | 2021 |
| Epsilon Home Ltd | Manufacturing | 2022 |
| Ergi & David Greek Gyros Shoreham Limited | Hospitality | 2024 |
| Ergi & David Property Development Ltd | Construction | 2025 |
| Ernest Air-Cooling Ltd | Energy | 2011 |
| Eslem Fish Bar Ltd | Hospitality | 2023 |
| Esprit Property Investments Limited | Real estate | 2019 |
| Essarr Holdings Limited | Financial services | 2025 |
| Essarr Ltd | Wholesale & retail | 2013 |
| Estuary Waterfront RTM Company Limited | Extraterritorial | 2025 |
| Evad Holdings Limited | Professional & scientific | 2009 |
| Evad Think Unified Limited | Administrative & support | 2009 |
| Evergreen Developments Limited | Real estate | 1980 |
| Every Wrong Direction CIC | Education | 2026 |
| EVM Physiotherapy Ltd | Health & social care | 2025 |
| Evolution Branding Limited | Manufacturing | 2013 |
| EX Veritas Limited | Administrative & support | 2005 |
| Ex-Tech Pioneer Limited | Financial services | 2023 |
| Executive Development Network Limited | Professional & scientific | 2020 |
| Expert Witnesses Limited | Extraterritorial | 2013 |
| Eyebrid Blaze Limited | Arts & recreation | 2006 |
| F Pickles Limited | Hospitality | 2025 |
| F9 Audio Ltd | Information & communication | 2015 |
| Face Aesthetics and Training Ltd | Health & social care | 2025 |
| Fairhall Electrical Brighton Limited | Construction | 2024 |
| Fairlawns Hove Limited | Households | 1988 |
| Fairview Solutions Ltd | Information & communication | 2023 |
| Fanakapan Limited | Arts & recreation | 2021 |
| Fanfusion Breakers Limited | Wholesale & retail | 2025 |
| FC Property Investments Ltd | Real estate | 2020 |
| Feetures Footcare Limited | Health & social care | 2007 |
| Fenicottero Ltd | Wholesale & retail | 2026 |
| Fern Bidco Limited | Financial services | 2024 |
| Fern Holdco Limited | Financial services | 2024 |
| Fern Midco Limited | Financial services | 2024 |
| Fern Place Development Ltd | Construction | 2006 |
| Fete N Lime Ltd | Hospitality | 2019 |
| Field House Lofts (FMC) Limited | Households | 2019 |
| Fifteen First Avenue (Hove) Limited | Households | 1959 |
| Filipino Venue Ltd | Hospitality | 2024 |
| Fircroft & Seventh Limited | Construction | 2019 |
| Firetel Ltd | Professional & scientific | 2004 |
| Firetoys Limited | Manufacturing | 2002 |
| First Line Cyber Resilience Ltd | Professional & scientific | 2024 |
| Fitness FAN Limited | Arts & recreation | 2015 |
| Fitz Facilities Management Ltd | Construction | 2024 |
| Five Goldsmid Road RTM Company Limited | Households | 2020 |
| Fletcher-Mclean Ltd | Real estate | 2013 |
| Flight Calibration Services Ltd | Professional & scientific | 2005 |
| Flint & Sons Ltd | Construction | 2021 |
| Flint Accounting Limited | Professional & scientific | 2021 |
| Flint Garden Studio Ltd | Administrative & support | 2024 |
| Floating Doctor Ltd | Health & social care | 2022 |
| Florence Road Events Limited | Hospitality | 2024 |
| Flying Lobster Aviation and Land Limited | Agriculture & fishing | 2019 |
| FMV Services Ltd | Construction | 2020 |
| Focus 4 U Ltd. | Information & communication | 2003 |
| Focus Group Trading Limited | Financial services | 2020 |
| Focus HUT Limited | Hospitality | 2019 |
| Focus Select Limited | Administrative & support | 2011 |
| Formula Performance Ltd | Education | 2018 |
| Foschini Property Ltd | Real estate | 2011 |
| Foureyes Design Ltd | Professional & scientific | 2006 |
| Frangipani Style Ltd | Wholesale & retail | 2010 |
| Frankling Estates Ltd | Real estate | 2021 |
| Fray Harvey Marketing & Communications Ltd | Professional & scientific | 2021 |
| Freedom Mobility Supplies Ltd. | Wholesale & retail | 2012 |
| Freemaison Productions Ltd | Information & communication | 2013 |
| Frenches Development Limited | Construction | 2025 |
| Fresh Art Club Ltd | Arts & recreation | 2024 |
| Frosts (Cars) Group Limited | Financial services | 2025 |
| Full Range Physiotherapy Limited | Extraterritorial | 2023 |
| Funky Dragon (Sussex) Limited | Hospitality | 2024 |
| Furze Croft Management Company Limited | Households | 1991 |
| Fusion Networks Ltd | Information & communication | 2015 |
| G3 Management Company Limited | Real estate | 2007 |