| S & S Robertson Ltd | Wholesale & retail | 2008 |
| S Johnston Services Ltd | Construction | 2013 |
| S Pottinger Ltd | Agriculture & fishing | 2020 |
| S. a. Laurenson Ltd | Other services | 2014 |
| S.M.S.I. Limited | Wholesale & retail | 2010 |
| S.Orlowski Ltd | Construction | 2025 |
| S.T.M. Shetland Ltd. | Wholesale & retail | 2004 |
| Saeter Fishing Ltd | Other services | 2020 |
| Safety Solutions Shetland Ltd | Professional & scientific | 2020 |
| Saffron Restaurant & Bar Ltd | Hospitality | 2019 |
| Sail Line Fish Ltd | Other services | 2009 |
| Sbsl Ltd | Other services | 2018 |
| SC691537 Limited | Administrative & support | 2021 |
| Scalloway Community Development Company Ltd | Hospitality | 2019 |
| Scalloway Enterprises Ltd | Hospitality | 2026 |
| Scalloway Hotel (2022) Limited | Hospitality | 2022 |
| Scalloway Meat Company Limited | Wholesale & retail | 2014 |
| Scatsta Investments Limited | Financial services | 2017 |
| Scholland Farm Ltd | Agriculture & fishing | 2015 |
| Scoop Digital Agency Ltd | Professional & scientific | 2015 |
| Scott Shellfish Ltd | Agriculture & fishing | 2013 |
| Scott Welding & Fabrication Limited | Manufacturing | 2014 |
| SCT Renewables Limited | Energy | 2003 |
| SE Harmer Limited | Construction | 2024 |
| Sea Shetland Ltd | Administrative & support | 2022 |
| Seafood 60 Degrees North Ltd. | Wholesale & retail | 2003 |
| Sean Dickson Ltd | Professional & scientific | 2013 |
| Seelaberg Services Ltd | Agriculture & fishing | 2012 |
| Selkie Ventures Ltd | Administrative & support | 2015 |
| Shandsmith Limited | Professional & scientific | 2019 |
| Shearer Engineering Ltd | Agriculture & fishing | 2022 |
| Shearer Fishing Co Ltd | Agriculture & fishing | 2011 |
| Shelhopkinsfitness Ltd | Other services | 2021 |
| Shetland Adventure Tours Ltd | Administrative & support | 2017 |
| Shetland Analytical Services EOT Trustee Limited | Financial services | 2025 |
| Shetland Arts IP C.I.C. | Information & communication | 2011 |
| Shetland Bookkeeping Services Ltd | Professional & scientific | 2016 |
| Shetland BUS and Coach Ltd | Transport & logistics | 2020 |
| Shetland Carbon Ltd | Administrative & support | 2019 |
| Shetland Chauffeur Company Ltd | Transport & logistics | 2025 |
| Shetland Chiropractic Clinic Limited | Health & social care | 2013 |
| Shetland Cleaning Company Ltd | Administrative & support | 2025 |
| Shetland Cleaning Services (Lerwick) Limited | Administrative & support | 1985 |
| Shetland Construction & Developments Limited | Construction | 2010 |
| Shetland Control Systems Limited | Manufacturing | 2006 |
| Shetland Diving Services Limited | Other services | 2008 |
| Shetland Events Ltd | Information & communication | 2026 |
| Shetland Exhaust & Tyre Centre Limited | Real estate | 1981 |
| Shetland Farm Dairies Limited | Manufacturing | 1994 |
| Shetland Food and Drink Limited | Other services | 2017 |
| Shetland Freezer Holdings Limited | Real estate | 1992 |
| Shetland FUR Babies Ltd | Agriculture & fishing | 2025 |
| Shetland GAS Supplies (UK) Ltd | Construction | 2010 |
| Shetland Glamping Ltd | Hospitality | 2021 |
| Shetland Heat Energy and Power Limited | Energy | 1998 |
| Shetland Hot Tubs Ltd | Other services | 2016 |
| Shetland Indoor Arena Ltd | Arts & recreation | 2019 |
| Shetland Industrial Cleaning Services Ltd | Administrative & support | 2021 |
| Shetland Industries Limited | Transport & logistics | 1988 |
| Shetland Islands Broadcasting Company Limited | Information & communication | 1985 |
| Shetland Jewellery Ltd | Manufacturing | 2021 |
| Shetland Link UP | Health & social care | 2004 |
| Shetland Lodges Ltd. | Hospitality | 2012 |
| Shetland Marine Limited | Agriculture & fishing | 2011 |
| Shetland Marineco Ltd | Professional & scientific | 2013 |
| Shetland MOT & Repair Limited | Wholesale & retail | 2015 |
| Shetland Nature Limited | Administrative & support | 2012 |
| Shetland Peatland Restoration Services Ltd | Other services | 2020 |
| Shetland Plant Hire Ltd | Construction | 2022 |
| Shetland Plant Limited | Construction | 2022 |
| Shetland Plant Maintenance Ltd | Manufacturing | 2022 |
| Shetland Power Tools Ltd | Wholesale & retail | 2014 |
| Shetland Precast Ltd | Wholesale & retail | 2019 |
| Shetland Property & Accommodation Ltd | Real estate | 2026 |
| Shetland Property Development Ltd | Construction | 2023 |
| Shetland Property Rentals Ltd | Real estate | 2013 |
| Shetland Refreshments Limited | Manufacturing | 1998 |
| Shetland Renovations Ltd | Construction | 2020 |
| Shetland Rural Experience Centre Ltd | Administrative & support | 2022 |
| Shetland Salt Limited | Manufacturing | 2019 |
| Shetland Seabird Tours Limited | Administrative & support | 2016 |
| Shetland Seafood Auctions Limited | Agriculture & fishing | 1998 |
| Shetland Speakeasy Limited | Manufacturing | 2023 |
| Shetland Tall Ships Ltd | Arts & recreation | 2008 |
| Shetland UHI | Professional & scientific | 2019 |
| Shetland Vets Trustees Limited | Professional & scientific | 2014 |
| Shetland Weathers LLP | | 2011 |
| Shetland With a Local Ltd | Administrative & support | 2025 |
| Shetland Wool Adventures Limited | Administrative & support | 2016 |
| Shetland's Finest Ltd | Wholesale & retail | 2010 |
| SHETLANDNO88 Ltd | Hospitality | 2025 |
| Shetnet Ltd | Information & communication | 2024 |
| Siam Harvest Ltd | Wholesale & retail | 2022 |
| Silicon Croft Limited | Information & communication | 2023 |
| Sirius Marine Ltd | Agriculture & fishing | 2017 |
| SJS Fishing Ltd | Agriculture & fishing | 2022 |
| Skylda Consulting Ltd | Professional & scientific | 2026 |
| Smartink Ltd | Other services | 2014 |
| Smith Building Contractors (Shetland) Ltd | Administrative & support | 2024 |
| SN Renewables Ltd | Other services | 2018 |