| Raruk Automation Limited | Manufacturing | 2015 |
| Raruk Holdings Limited | Professional & scientific | 2011 |
| Raymac Properties Ltd | Real estate | 1998 |
| Raymech Limited | Energy | 1993 |
| Rcmf Ltd | Hospitality | 2024 |
| Reconditioning Services Limited | Wholesale & retail | 2011 |
| Redstop Limited | Wholesale & retail | 2020 |
| Refinish Centre Shefford Ltd | Wholesale & retail | 2019 |
| Relation Motors Limited | Wholesale & retail | 2025 |
| Renaissance Computers and Consultancy Limited | Information & communication | 1981 |
| Resilient Roots Services Ltd | Health & social care | 2026 |
| Respond Logistics Ltd | Administrative & support | 2002 |
| Rethink IT Consulting Ltd | Information & communication | 2026 |
| REV & Ride Motorbike Ltd | Arts & recreation | 2025 |
| RGM Truck & Van Ltd | Wholesale & retail | 2018 |
| Ridlow Properties Limited | Real estate | 2025 |
| Right Cars 4U Limited | Wholesale & retail | 2025 |
| River Dreams Ltd | Other services | 2013 |
| RJ Pharma Limited | Professional & scientific | 2025 |
| RJD Autos Ltd | Wholesale & retail | 2018 |
| RK Prestige Ltd | Real estate | 2025 |
| RKK Childcare Ltd | Health & social care | 2024 |
| RKY Under Pressure Ltd | Administrative & support | 2025 |
| ROB Bryan Associates Ltd | Administrative & support | 2007 |
| Robinshire Ltd | Real estate | 2022 |
| Robinson Grove Management Company Limited | Real estate | 2019 |
| Rooflight Factory Limited | Wholesale & retail | 2024 |
| Rosetone Contract Furniture Limited | Wholesale & retail | 2019 |
| Royston Tyres & Exhaust Centre Limited | Wholesale & retail | 2016 |
| RPB Electrical Services Ltd | Construction | 2019 |
| RPT Investments Limited | Real estate | 2022 |
| Rsjc Limited | Arts & recreation | 2015 |
| Russell Heating Ltd | Construction | 2010 |
| S & R Ford Properties Limited | Real estate | 2014 |
| S B Franchise Ltd | Administrative & support | 2023 |
| S Barbering Ltd | Other services | 2020 |
| Safety First on Site Ltd | Construction | 2025 |
| SAM Allen Ltd | Construction | 2018 |
| Samurai Computing Ltd | Information & communication | 1997 |
| Santosh and Kavita Limited | Wholesale & retail | 2017 |
| SC & Partners Ltd | Professional & scientific | 2022 |
| School Hire & Functions Limited | Education | 2010 |
| Selexic Limited | Administrative & support | 2019 |
| Semms Fabrications Ltd | Manufacturing | 2007 |
| Sentiacards Ltd | Wholesale & retail | 2026 |
| Seventen Group Limited | Financial services | 2022 |
| Seventen Properties Limited | Construction | 2023 |
| SG Spice Ltd | Hospitality | 2018 |
| SGD Carpentry & Building Services Limited | Construction | 2022 |
| Shanti Warrior Yoga Limited | Arts & recreation | 2013 |
| Shefford Accountancy Services Limited | Professional & scientific | 2014 |
| Shefford Autos Limited | Wholesale & retail | 2025 |
| Shefford Convenience Store Limited | Wholesale & retail | 2016 |
| Shefford Dental Practice Limited | Health & social care | 2012 |
| Shefford Electrical Ltd | Construction | 2023 |
| Shefford Landscaping & Groundworks Ltd | Administrative & support | 2022 |
| Shefford Nails Studio Ltd | Other services | 2025 |
| Shefford Paint Works Limited | Wholesale & retail | 2024 |
| Shefford Tyres & Exhaust Centre Limited | Wholesale & retail | 2016 |
| Sitek Signs Limited | Professional & scientific | 2020 |
| Skpc Properties Ltd | Real estate | 2018 |
| SM Tennis Coaching Ltd | Education | 2023 |
| Smdl Ltd | Real estate | 2022 |
| Smith Companion Pets Ltd. | Wholesale & retail | 2017 |
| Smithfield Promotions Limited | Extraterritorial | 2003 |
| South Paddock Clifton Management Limited | Households | 2017 |
| Southfields 1967 Limited | Extraterritorial | 2014 |
| Southfields Court Property Management Limited | Real estate | 2005 |
| Spark and Hertz Electrical Ltd | Construction | 2018 |
| Sparks of Shefford Limited | Construction | 2023 |
| Speeds Dairy Barns Management Limited | Households | 2009 |
| Spencer Wilkinson Holdings Limited | Financial services | 2025 |
| Spidey Web Design Ltd | Information & communication | 2015 |
| Spirit-Bred Praise House Ltd | Other services | 2025 |
| Splitt Mortgages and Protection Limited | Financial services | 2025 |
| SPO Business Consultancy Limited | Professional & scientific | 2024 |
| Spraynet Ltd | Construction | 2026 |
| Squires Park Management Limited | Households | 2007 |
| ST Finians Services Ltd | Professional & scientific | 2022 |
| ST Helen Holdings Ltd | Financial services | 2024 |
| Standby Coffee Ltd | Manufacturing | 2022 |
| Status Homes Limited | Construction | 2012 |
| Step Into Your Power Limited | Health & social care | 2026 |
| Steve Dear Tree Services Limited | Other services | 2006 |
| Steven Foot Builders Ltd | Construction | 2025 |
| Stocker Interiors Limited | Professional & scientific | 2025 |
| Storm Electrics Ltd | Construction | 2011 |
| Streamworx Limited | Extraterritorial | 2020 |
| Stretchband Films Ltd | Wholesale & retail | 2022 |
| Strictly Property Group Limited | Real estate | 2023 |
| Strictly Tables and Chairs Limited | Wholesale & retail | 2008 |
| Structec Ltd | Construction | 2018 |
| SWP Catering Services Ltd | Hospitality | 2019 |
| Synergy Social Ltd | Professional & scientific | 2026 |
| T & H Mitchell Ltd | Hospitality | 2017 |
| T Chapman Bathrrooms & Kitchens Ltd | Other services | 2019 |
| T J Plumbing Shefford Limited | Construction | 2016 |
| T Motor Limited | Wholesale & retail | 2020 |
| Taay Ltd | Administrative & support | 2020 |
| TAP Food Ltd | Hospitality | 2023 |