| 1-3 Shepherds Place RTM Company Limited | Households | 2023 |
| 104 Clifton Management Company Ltd | Households | 2014 |
| 148 Clifton Road Management Company Limited | Professional & scientific | 2007 |
| 36 Flitwick Road Ltd | Construction | 2025 |
| 3I Technical Consulting Ltd | Professional & scientific | 2019 |
| 84 Fildyke Road Ltd | Households | 2026 |
| A Plus Health Ltd | Public administration | 2023 |
| A Way to Makkah HAJ & Umrah Services Ltd | Administrative & support | 2016 |
| A&G Digital Ltd | Information & communication | 2025 |
| A&G Synergy Ltd | Information & communication | 2024 |
| A.S.M Fire Solutions Ltd | Construction | 2026 |
| A2Z Windows and Doors Limited | Wholesale & retail | 2016 |
| Academy Labs Ltd | Information & communication | 1986 |
| ACO Academy Ltd | Professional & scientific | 2007 |
| ACO Building Drainage Ltd | Professional & scientific | 2004 |
| ACO Fulbora Limited | Professional & scientific | 1992 |
| ACO Polymer Products Ltd. | Professional & scientific | 1993 |
| ACO Technologies PLC | Manufacturing | 1984 |
| ACO Wildlife Limited | Professional & scientific | 1984 |
| Acoline Softeners Ltd | Other services | 2011 |
| Acreations Ltd | Extraterritorial | 2024 |
| Acronym Limited | Mining & quarrying | 2001 |
| Active Commercial Services Limited | Professional & scientific | 2019 |
| Active Support Clifton Ltd | Health & social care | 2021 |
| Active.Syncro Ltd | Wholesale & retail | 2026 |
| Adept Fabrications Limited | Manufacturing | 2004 |
| AEG Climate Solutions Ltd | Construction | 2024 |
| Aeroclubs Ltd | Arts & recreation | 2021 |
| Aeroy Ltd | Information & communication | 2020 |
| Aesthetic Sweetheart Ltd | Extraterritorial | 2024 |
| AGK R US Ltd | Other services | 2007 |
| AGR Tree Surveys Ltd | Agriculture & fishing | 2023 |
| Agri-Tech Services (UK) Limited | Agriculture & fishing | 2006 |
| Agricultural Bird Control Ltd | Agriculture & fishing | 2026 |
| Aitch-Electrical Ltd | Construction | 2023 |
| Albone Biddle & Partners Ltd | Construction | 2023 |
| Aljec Ltd | Wholesale & retail | 2003 |
| Allied Assessment Holdings Limited | Professional & scientific | 2022 |
| Allsopp Property Services Limited | Construction | 2023 |
| Alpha Biopharma Consulting Ltd | Professional & scientific | 2025 |
| Alphabet Barn Limited | Wholesale & retail | 2021 |
| AM Rope Access & Training Ltd | Other services | 2020 |
| Ambersley Limited | Construction | 1997 |
| Amoeba Limited | Financial services | 1991 |
| Andrew Prime Ltd | Other services | 2019 |
| Animo Business Consulting Ltd | Professional & scientific | 2019 |
| Annilytics Consultancy Ltd | Professional & scientific | 2021 |
| Antecha Ltd | Professional & scientific | 2014 |
| Appledale Croft Limited | Real estate | 1994 |
| Arden Project Management Limited | Construction | 2003 |
| Ardour Equestrian Ltd | Wholesale & retail | 2023 |
| ARF Systems Ltd | Information & communication | 2010 |
| Arnold & Sharp 2015 Limited | Real estate | 2015 |
| Arru Homes Ltd | Real estate | 2025 |
| Arty Amber Limited | Education | 2023 |
| AS Pro Sports Ltd | Wholesale & retail | 2023 |
| ASG Scenery Limited | Information & communication | 2021 |
| Ashden Developments Limited | Professional & scientific | 2009 |
| Askco Plumbing & Heating Ltd | Construction | 2019 |
| Aston Talent Agency Ltd | Information & communication | 2024 |
| Attitude Bikes Ltd | Wholesale & retail | 2019 |
| Auto Performance Services Ltd | Wholesale & retail | 2023 |
| Avax Suction Ltd | Water & waste | 2022 |
| Aventus Services Limited | Construction | 2018 |
| Aviamics Limited | Professional & scientific | 2008 |
| Avrit PVT. Limited | Real estate | 2022 |
| AWE Interactive Limited | Information & communication | 2019 |
| B.Nicholls Plumbing and Heating Ltd | Construction | 2026 |
| B.W. Evans Limited | Information & communication | 1998 |
| BA Mooves Services Ltd | Real estate | 2019 |
| Bacha Khan Bazaar Ltd | Wholesale & retail | 2020 |
| Baldock Car Sales Limited | Wholesale & retail | 2017 |
| Barnwell Music Ltd | Arts & recreation | 2019 |
| Baystrait Limited | Administrative & support | 1997 |
| BB Family Limited | Education | 2025 |
| BCS Heating & Plumbing Limited | Construction | 2007 |
| Be-Clean Solutions Ltd | Administrative & support | 2024 |
| Beadlow Motors Ltd | Wholesale & retail | 2020 |
| Bear Plumbing, Heating and Tiling. Ltd | Construction | 2023 |
| Becky Timmons Ltd | Professional & scientific | 2025 |
| Bedfordshire East Schools Trust Limited | Education | 2009 |
| Bedfordshire East Schools Trust Nurseries Ltd | Education | 2011 |
| Bedfordshire Schools Trust Limited | Education | 2011 |
| Beechwood Real Estate Ltd | Real estate | 2021 |
| BEN Marshall Electrical Ltd | Construction | 2019 |
| BEN Smith (JNR) Limited | Construction | 2018 |
| Benchmark Internet Limited | Information & communication | 2022 |
| Bentleys Accountancy Limited | Administrative & support | 2009 |
| Berti Excavation Ltd | Construction | 2024 |
| Best Plumbing Supplies Limited | Wholesale & retail | 2024 |
| Bethel Property Holdings Ltd | Real estate | 2020 |
| Beverley Gardens Limited | Real estate | 2010 |
| Biolipid Limited | Professional & scientific | 2009 |
| Biomeasurements Limited | Agriculture & fishing | 2005 |
| Blaxsell Financial Planning Ltd | Financial services | 2021 |
| Bliss Fitness Ltd | Health & social care | 2020 |
| Blossom Beauty Salon Shefford Limited | Other services | 2018 |
| Bluebell Lily Estates Limited | Real estate | 2025 |
| Bluewater Valves Limited | Administrative & support | 2009 |
| Bookandtrain.Com Limited | Education | 2014 |